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01-01-2008 | Original article

Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine

Authors: Youssef Mir, Daniel Houde, Johan E. van Lier

Published in: Lasers in Medical Science | Issue 1/2008

Abstract

Sequential two-photon (2-γ) activated copper tetrasulfophthalocyanine (CuPcS₄) was shown capable of inactivating acetylcholinesterase (ACE). ACE activity was measured photometrically by the Ellman method. Simultaneous irradiation of ACE in the presence of CuPcS₄ with 514 nm (183 mW/cm²) and 670 nm (86 mW/cm²) continuous wave (CW) light induced a 20–50% increase in enzyme inhibition as compared to one-photon (1-γ) irradiation, using either 514- or 670-nm (CW) light at the same fluences. The enzyme activity was not affected by CuPcS₄ or light alone, decreased linearly with the irradiation time, and was shown to be oxygen-dependent. We conclude that the photoinactivation of ACE with sequential 2-γ irradiation involves reactive oxygen species produced by the interaction of the upper excited T_n state of CuPcS₄ with molecular oxygen. As CuPcS₄ shows little activity as a conventional 1-γ photosensitizer, unwanted side effects such as prolonged skin sensitivity are eliminated rendering 2-γ photodynamic therapy advantageous for the treatment of selected medical applications.

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Title: Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine
Authors: Youssef Mir
Daniel Houde
Johan E. van Lier
Publication date: 01-01-2008
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 1/2008
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-007-0446-x

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Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine

Abstract

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Abstract

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