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Lasers in Medical Science
Published in: Lasers in Medical Science 9/2016

01-12-2016 | Original Article

Photobiomodulation therapy on collagen type I and III, vascular endothelial growth factor, and metalloproteinase in experimentally induced tendinopathy in aged rats

Authors: Anna Cristina de Farias Marques, Regiane Albertini, Andrey Jorge Serra, Evela Aparecida Pereira da Silva, Vanessa Lima Cavalcante de Oliveira, Luciana Miatto Silva, Ernesto Cesar Pinto Leal-Junior, Paulo de Tarso Camillo de Carvalho

Published in: Lasers in Medical Science | Issue 9/2016

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Abstract

This study investigates the effect of photobiomodulation therapy (PBMT) on collagen type I and III, matrix metalloproteinase (MMP), and vascular endothelial growth factor (VEGF) in experimentally induced tendinopathy in female aged rats. Tendinopathy was induced by the Achilles tendoncollagenase peritendinous. Forty-two Wistar rats (Norvegicus albinus) were used; groups consisted of 36 aged animals (18 months old; mean body weight, 517.7 ± 27.54 g) and 6 adult animals (12 weeks old; mean body weight, 266± 19.30 g). The animals were divided into three groups: control, aged tendinopathy, and aged tendinopathy PBMT; the aged groups were subdivided based on time to euthanasia: 7, 14, and 21 days. PBMT involved a gallium-arsenide-aluminum laser (Theralaser, DMC®) with active medium operating at wavelength 830 ± 10 nm, 50 mW power, 0.028 cm2 laser beam, 107 J/cm2 energy density, 1.8 W/cm2 power density, and an energy of 3 J per point. The laser was applied by direct contact with the left Achilles tendon during 60 s per point at a frequency of three times per week, until the euthanasia date (7, 14, and 21 days). VEGF, MMP-3, and MMP-9 were analyzed by immunohistochemistry, and collagen type I and III by Sirius red. PBMT increased the deposition of collagen type I and III in a gradual manner, with significant differences relative to the group aged tendonitis (p < 0.001), and in relation to VEGF (p < 0.001); decreased expression of MMP-3 and 9 were observed in group aged tendinopathy (p < 0.001). PBMT, therefore, increased the production of collagen type I and III, downregulated the expression of MMP-3 and MMP-9, and upregulated that of VEGF, with age and age-induced hormonal deficiency.
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Metadata
Title
Photobiomodulation therapy on collagen type I and III, vascular endothelial growth factor, and metalloproteinase in experimentally induced tendinopathy in aged rats
Authors
Anna Cristina de Farias Marques
Regiane Albertini
Andrey Jorge Serra
Evela Aparecida Pereira da Silva
Vanessa Lima Cavalcante de Oliveira
Luciana Miatto Silva
Ernesto Cesar Pinto Leal-Junior
Paulo de Tarso Camillo de Carvalho
Publication date
01-12-2016
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 9/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-016-2070-0

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