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Lasers in Medical Science
Published in: Lasers in Medical Science 3/2019

01-04-2019 | Original Article

Photobiomodulation is associated with a decrease in cell viability and migration in oral squamous cell carcinoma

Authors: Tatiana Dias Schalch, Maria Helena Fernandes, Maria Fernanda Setúbal Destro Rodrigues, Douglas Magno Guimarães, Fabio Daumas Nunes, João Costa Rodrigues, Mônica Pereira Garcia, Raquel Agnelli Mesquita Ferrari, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes

Published in: Lasers in Medical Science | Issue 3/2019

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Abstract

The treatment of squamous cell carcinoma (SCC) involves surgery, chemotherapy, and/or radiotherapy, which can cause mucositis (inflammation of the oral mucosa that causes considerable pain and can compromise the continuity of oncological treatment). Photobiomodulation (PBM) has been successfully used in the treatment of mucositis, but doubts arise regarding the use of laser for areas in which tumor cells may remain. In this study, the effect of PBM on the viability, mitochondrial activity, proliferation, apoptosis, and migration of cells derived from oral SCC was evaluated. SCC9 cells were irradiated with laser (660 and 780 nm, using 11 dosimetric parameters) and submitted to mitochondrial and caspase 3 activity tests after 1 and 3 days. Based on the results, cell viability (neutral red assay), proliferation (BrdU assay), and migration (scratch-wound assay) were evaluated using only the dosimetric parameters recommended for mucositis. Non-irradiated cells served as the control. The experiments were performed in triplicate. The 11 parameters diminished mitochondrial activity and induced tumor cell apoptosis. Using the parameters recommended for mucositis, irradiation with 780 nm (70 mW, 4 J/cm2) proved to be the safest and led to a reduction in cell viability, the induction of apoptosis, and a reduction in the migration capacity of the tumor cells.
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Metadata
Title
Photobiomodulation is associated with a decrease in cell viability and migration in oral squamous cell carcinoma
Authors
Tatiana Dias Schalch
Maria Helena Fernandes
Maria Fernanda Setúbal Destro Rodrigues
Douglas Magno Guimarães
Fabio Daumas Nunes
João Costa Rodrigues
Mônica Pereira Garcia
Raquel Agnelli Mesquita Ferrari
Sandra Kalil Bussadori
Kristianne Porta Santos Fernandes
Publication date
01-04-2019
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 3/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-018-2640-4

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