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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 5/2014

01-05-2014 | Breast Oncology

Photoacoustic and Fluorescence Image-Guided Surgery Using a Multifunctional Targeted Nanoprobe

Authors: Lei Xi, PhD, Guangyin Zhou, PhD, Ning Gao, PhD, Lily Yang, MD, PhD, David A. Gonzalo, MD, Steven J. Hughes, MD, Huabei Jiang, PhD

Published in: Annals of Surgical Oncology | Issue 5/2014

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Abstract

Purpose

A complete surgical excision with negative tumor margins is the single most important factor in the prediction of long-term survival for most cancer patients with solid tumors. We hypothesized that image-guided surgery using nanoparticle-enhanced photoacoustic and fluorescence imaging could significantly reduce the rate of local recurrence.

Methods

A murine model of invasive mammary carcinoma was utilized. Three experimental groups were included: (1) control; (2) tumor-bearing mice injected with non-targeted nanoprobe; and (3) tumor-bearing mice injected with targeted nanoprobe. The surgeon removed the primary tumor following the guidance of photoacoustic imaging (PAI), then inspected the surgical wound and removed the suspicious tissue using intraoperative near-infrared (NIR) fluorescence imaging. The mice were followed with bioluminescence imaging weekly to quantify local recurrence.

Results

Nanoprobe-enhanced photoacoustic contrast enabled PAI to map the volumetric tumor margins up to a depth of 31 mm. The targeted nanoparticles provided significantly greater enhancement than non-targeted nanoparticles. Seven mice in the group injected with the targeted nanoprobes underwent additional resections based upon NIR fluorescence imaging. Pathological analysis confirmed residual cancer cells in the re-resected specimens in 5/7 mice. Image-guided resection resulted in a significant reduction in local recurrence; 8.7 and 33.3 % of the mice in the targeted and control groups suffered recurrence, respectively.

Conclusions

These results suggest that photoacoustic and NIR intraoperative imaging can effectively assist a surgeon to locate primary tumors and to identify residual disease in real-time. This technology has promise to overcome current clinical challenges that result in the need for second surgical procedures.
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Metadata
Title
Photoacoustic and Fluorescence Image-Guided Surgery Using a Multifunctional Targeted Nanoprobe
Authors
Lei Xi, PhD
Guangyin Zhou, PhD
Ning Gao, PhD
Lily Yang, MD, PhD
David A. Gonzalo, MD
Steven J. Hughes, MD
Huabei Jiang, PhD
Publication date
01-05-2014
Publisher
Springer US
Published in
Annals of Surgical Oncology / Issue 5/2014
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-014-3541-9

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