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01-12-2018 | Brief Report

Performance evaluation of a newly developed molecular assay for the accurate diagnosis of gastroenteritis associated with norovirus of genogroup II

Authors: Simona De Grazia, Floriana Bonura, Vincenzo Cappa, Sara Li Muli, Arcangelo Pepe, Noemi Urone, Giovanni M. Giammanco

Published in: Archives of Virology | Issue 12/2018

Abstract

The performance of a newly proposed fully automated cassette-based sample-to-results solution for norovirus (NoV) detection, InGenius Norovirus ELITe MGB®, was evaluated. A total of 120 selected archival stool samples from children hospitalized for acute gastroenteritis were used to compare the results to a reference real-time RT-PCR. The InGenius NoV assay showed optimal diagnostic accuracy (sensitivity, 100%; specificity, 95.7%) and was able to correctly detect the entire wide panel of epidemiologically relevant genotypes tested. These preliminary results suggest that the InGenius NoV assay can be recommended as a valuable method for accurate diagnosis of NoV GII infection in epidemic and sporadic gastroenteritis.

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Title: Performance evaluation of a newly developed molecular assay for the accurate diagnosis of gastroenteritis associated with norovirus of genogroup II
Authors: Simona De Grazia
Floriana Bonura
Vincenzo Cappa
Sara Li Muli
Arcangelo Pepe
Noemi Urone
Giovanni M. Giammanco
Publication date: 01-12-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 12/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-4010-8

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