Skip to main content
Top
Published in: Archives of Virology 9/2008

01-09-2008 | Brief Report

Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana

Authors: O. Netsu, K. Hiratsuka, S. Kuwata, T. Hibi, M. Ugaki, M. Suzuki

Published in: Archives of Virology | Issue 9/2008

Login to get access

Abstract

To analyze the role of the 2b protein (2bP) of Peanut stunt virus (PSV) in the viral infection cycle, we constructed PSV mutants that express either no 2bP or N-terminal-truncated 2bP. The accumulation of wild-type and mutant viruses in tobacco protoplasts indicated that the 2b cistron is not essential for viral replication. Viral accumulation in Nicotiana benthamiana plants suggested that the 2b cistron is responsible for viral accumulation in inoculated and upper leaves and has a role in virulence. The involvement of eight N-terminal amino acids of 2bP in these functions is discussed.
Literature
1.
go back to reference Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17:6739–6746PubMedCrossRef Brigneti G, Voinnet O, Li WX, Ji LH, Ding SW, Baulcombe DC (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J 17:6739–6746PubMedCrossRef
2.
go back to reference Ding SW, Anderson BJ, Haase HR, Symons RH (1994) New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601PubMedCrossRef Ding SW, Anderson BJ, Haase HR, Symons RH (1994) New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601PubMedCrossRef
3.
go back to reference Ding SW, Li WX, Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J 14:5762–5772PubMed Ding SW, Li WX, Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J 14:5762–5772PubMed
4.
go back to reference Ding SW, Shi BJ, Li WX, Symons RH (1996) An interspecies hybrid RNA virus is significantly more virulent than either parental virus. Proc Natl Acad Sci USA 93:7470–7474PubMedCrossRef Ding SW, Shi BJ, Li WX, Symons RH (1996) An interspecies hybrid RNA virus is significantly more virulent than either parental virus. Proc Natl Acad Sci USA 93:7470–7474PubMedCrossRef
5.
go back to reference Hayes RJ, Buck KW (1990) Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368PubMedCrossRef Hayes RJ, Buck KW (1990) Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63:363–368PubMedCrossRef
6.
go back to reference Hu CC, Aboul-Ata AE, Naidu RA, Ghabrial SA (1997) Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA 3. J Gen Virol 78:929–939PubMed Hu CC, Aboul-Ata AE, Naidu RA, Ghabrial SA (1997) Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA 3. J Gen Virol 78:929–939PubMed
7.
go back to reference Hu CC, Sanger M, Ghabrial SA (1998) Production of infectious RNA transcripts from full-length cDNA clones representing two subgroups of peanut stunt virus strains: mapping satellite RNA support to RNA 1. J Gen Virol 79:2013–2021PubMed Hu CC, Sanger M, Ghabrial SA (1998) Production of infectious RNA transcripts from full-length cDNA clones representing two subgroups of peanut stunt virus strains: mapping satellite RNA support to RNA 1. J Gen Virol 79:2013–2021PubMed
8.
go back to reference Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1991) Nucleotide sequence of RNA 3 of peanut stunt cucumovirus. Virology 185:464–467PubMedCrossRef Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1991) Nucleotide sequence of RNA 3 of peanut stunt cucumovirus. Virology 185:464–467PubMedCrossRef
9.
go back to reference Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1992) Nucleotide sequence analysis of peanut stunt cucumovirus RNAs 1 and 2. J Gen Virol 73:701–707PubMedCrossRef Karasawa A, Nakaho K, Kakutani T, Minobe Y, Ehara Y (1992) Nucleotide sequence analysis of peanut stunt cucumovirus RNAs 1 and 2. J Gen Virol 73:701–707PubMedCrossRef
10.
go back to reference Lu R, Folimonov A, Shintaku M, Li WX, Falk BW, Dawson WO, Ding SW (2004) Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci USA 101:15742–15747PubMedCrossRef Lu R, Folimonov A, Shintaku M, Li WX, Falk BW, Dawson WO, Ding SW (2004) Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome. Proc Natl Acad Sci USA 101:15742–15747PubMedCrossRef
11.
go back to reference Lucy AP, Guo HS, Li WX, Ding SW (2000) Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. EMBO J 19:1672–1680PubMedCrossRef Lucy AP, Guo HS, Li WX, Ding SW (2000) Suppression of post-transcriptional gene silencing by a plant viral protein localized in the nucleus. EMBO J 19:1672–1680PubMedCrossRef
12.
go back to reference Nitta N, Takanami Y, Kuwata S, Kubo S (1988) Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J Gen Virol 69:2695–2700CrossRef Nitta N, Takanami Y, Kuwata S, Kubo S (1988) Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J Gen Virol 69:2695–2700CrossRef
14.
go back to reference Shi BJ, Ding SW, Symons RH (1997) In vivo expression of an overlapping gene encoded by the cucumoviruses. J Gen Virol 78:237–241PubMed Shi BJ, Ding SW, Symons RH (1997) In vivo expression of an overlapping gene encoded by the cucumoviruses. J Gen Virol 78:237–241PubMed
15.
go back to reference Sivakumaran K, Chen MH, Roossinck MJ, Kao CC (2002) Core promoter for initiation of Cucumber mosaic virus subgenomic RNA 4A. Mol Plant Pathol 3:43–52CrossRef Sivakumaran K, Chen MH, Roossinck MJ, Kao CC (2002) Core promoter for initiation of Cucumber mosaic virus subgenomic RNA 4A. Mol Plant Pathol 3:43–52CrossRef
16.
go back to reference Soards AJ, Murphy AM, Palukaitis P, Carr JP (2002) Virulence and differential local and systemic spread of Cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Mol Plant Microbe Interact 15:647–653PubMedCrossRef Soards AJ, Murphy AM, Palukaitis P, Carr JP (2002) Virulence and differential local and systemic spread of Cucumber mosaic virus in tobacco are affected by the CMV 2b protein. Mol Plant Microbe Interact 15:647–653PubMedCrossRef
17.
go back to reference Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y (1991) Functional analysis of deletion mutants of cucumber mosaic virus RNA 3 using an in vitro transcription system. Virology 183:106–113PubMedCrossRef Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y (1991) Functional analysis of deletion mutants of cucumber mosaic virus RNA 3 using an in vitro transcription system. Virology 183:106–113PubMedCrossRef
18.
go back to reference Suzuki M, Kuwata S, Masuta C, Takanami Y (1995) Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco. J Gen Virol 76:1791–1799PubMedCrossRef Suzuki M, Kuwata S, Masuta C, Takanami Y (1995) Point mutations in the coat protein of cucumber mosaic virus affect symptom expression and virion accumulation in tobacco. J Gen Virol 76:1791–1799PubMedCrossRef
19.
go back to reference Suzuki M, Yoshida M, Yoshinuma T, Hibi T (2003) Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus. J Gen Virol 84:1931–1939PubMedCrossRef Suzuki M, Yoshida M, Yoshinuma T, Hibi T (2003) Interaction of replicase components between Cucumber mosaic virus and Peanut stunt virus. J Gen Virol 84:1931–1939PubMedCrossRef
20.
go back to reference Takeshita M, Suzuki M, Kuwata S, Takanami Y (1998) Involvement of cucumber mosaic virus RNA 2 and RNA 3 in viral systemic spread in radish plant. Arch Virol 143:1109–1117PubMedCrossRef Takeshita M, Suzuki M, Kuwata S, Takanami Y (1998) Involvement of cucumber mosaic virus RNA 2 and RNA 3 in viral systemic spread in radish plant. Arch Virol 143:1109–1117PubMedCrossRef
21.
go back to reference Takeshita M, Suzuki M, Takanami Y (2001) Combination of amino acids in the 3a protein and the coat protein of Cucumber mosaic virus determines symptom expression and viral spread in bottle gourd. Arch Virol 146:697–711PubMedCrossRef Takeshita M, Suzuki M, Takanami Y (2001) Combination of amino acids in the 3a protein and the coat protein of Cucumber mosaic virus determines symptom expression and viral spread in bottle gourd. Arch Virol 146:697–711PubMedCrossRef
22.
go back to reference Tsuchizaki T (1973) Peanut stunt virus isolated from beans in Japan. Ann Phytopath Soc Jpn 39:67–72 Tsuchizaki T (1973) Peanut stunt virus isolated from beans in Japan. Ann Phytopath Soc Jpn 39:67–72
23.
go back to reference Yan LY, Xu ZY, Goldbach R, Kunrong C, Prins M (2005) Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China. Arch Virol 150:1203–1211PubMedCrossRef Yan LY, Xu ZY, Goldbach R, Kunrong C, Prins M (2005) Nucleotide sequence analyses of genomic RNAs of Peanut stunt virus Mi, the type strain representative of a novel PSV subgroup from China. Arch Virol 150:1203–1211PubMedCrossRef
24.
go back to reference Zhang X, Yuan YR, Pei Y, Lin SS, Tuschl T, Patel DJ, Chua NH (2006) Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes Dev 20:3255–3268PubMedCrossRef Zhang X, Yuan YR, Pei Y, Lin SS, Tuschl T, Patel DJ, Chua NH (2006) Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes Dev 20:3255–3268PubMedCrossRef
25.
go back to reference Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucl Acids Res 31:3406–3415PubMedCrossRef Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucl Acids Res 31:3406–3415PubMedCrossRef
Metadata
Title
Peanut stunt virus 2b cistron plays a role in viral local and systemic accumulation and virulence in Nicotiana benthamiana
Authors
O. Netsu
K. Hiratsuka
S. Kuwata
T. Hibi
M. Ugaki
M. Suzuki
Publication date
01-09-2008
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2008
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-008-0166-y

Other articles of this Issue 9/2008

Archives of Virology 9/2008 Go to the issue
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine