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Comparative Clinical Pathology
Published in: Comparative Clinical Pathology 6/2019

01-12-2019 | Pancreatitis | Original Article

DGGR-lipase for effective diagnosis of pancreatitis in horses

Authors: Jessica P. Johnson, John D. Stack, Charlotte L. McGivney, Michael P. Garrett, Peter J. O’Brien

Published in: Comparative Clinical Pathology | Issue 6/2019

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Abstract

Equine pancreatitis has no reliable ante-mortem test and is considered rare. The 1,2-o-dilauryl-rac-glycero-3-glutarate-(6′-methylresorufin) ester (DGGR) lipase assay is specific for pancreatic lipase (PL). It was developed for humans and validated in dogs, cats and rats as specific for pancreatitis diagnosis. We used a novel validation strategy to confirm this tissue specificity extended to horses and determine reference ranges and pancreatitis prevalence. One hundred thirty-nine horses were used. Biopsies were collected from 3 healthy cadavers and venous blood from 109 clinical cases and 27 training racehorses. DGGR-lipase was demonstrated to only occur significantly in the pancreas using 7 different tissues collected at euthanasia that were cryopreserved and assayed for PL and 6, other, routine biomarkers (amylase, CK, GLD, AST, ALT, ALP). PL range was determined in healthy horses and in pancreatitis cases. PL is an inexpensive, highly-effective biomarker with the highest tissue activity and specificity of routine biomarkers. Equine pancreatitis occurs with a high prevalence and severity similar to that in dogs and cats, and in association with a wide variety of gastrointestinal disorders and with training racehorses. PL should be routinely assayed in horses with gastrointestinal disorders and monitored in training racehorses.
Footnotes
1
Polytron® PT2100, Kinematica, Luzern, Schweiz, Switzerland.
 
2
Randox RX Imola® analyser, Crumlin, Northern Ireland.
 
3
Advia 2120 haematology analyser, Siemens, Dublin, Ireland.
 
4
KC4 Delta coagulation analyser, Amelung, Germany.
 
5
GraphPad Prism, La Jolla, CA, USA.
 
Literature
Bakos Z, Krahcsovics L, Toth J (2008) Successful medical treatment of acute pancreatitis in a horse. Vet Rec 162:95–96CrossRef
Bourne Y, Martinez C, Kerfelec B, Lombardo D, Chapus C, Cambillau C (1994) Horse pancreatic lipase. The crystal structure refined at 2.3 Å resolution. J Mol Biol 238:709–732CrossRef
Clampitt RB, Hart RJ (1978) The tissue activities of some diagnostic enzymes in ten mammalian species. J Comp Pathol 88:607–621CrossRef
Dehghani SN, Tadjalli M, Seifali A (2005) Sialography in horse: technique and normal appearance. Vet Archiv 75:531–540
Embleton JK, Poutonb CW (1997) Clin Biochem 38:365–370. Structure and function of gastro-intestinal lipases. Adv Drug Deliv Rev 25:15–32
Furr MO, Robertson J (1992) Two cases of equine pancreatic disease and a review of the literature. Eq Vet Edu 4:55–58CrossRef
Grulke S, Gangl M, Deby-Dupont G et al (2002) Plasma trypsin level in horses suffering from acute intestinal obstruction. Vet J 63:283–291CrossRef
Grulke S, Deby-Dupont G, Cassart D, Gangly M, Caudron I, Lamy M, Serteyn D (2003) Pancreatic injury in equine acute abdomen evaluated by plasma trypsin activity and histopathology of pancreatic tissue. Vet Pathol 40:8–13CrossRef
Harris VD, Gow WJ (1892) Ferment actions of the pancreas in different animals. J Physiol 13:469–492CrossRef
Johnson PJ, Wiedmeyer CE, Messer NT (2009) Conditions of the equine pancreas. Eq Vet Edu 21:26–29CrossRef
Kekfelec B, Foglizzo E, Bonicel J et al (1992) Sequence of horse pancreatic lipase as determined by protein and cDNA sequencing. Implications for p-nitrophenyl acetate hydrolysis by pancreatic lipases. Eur J Biochem 206:279–287CrossRef
Lilley CW, Beeman GM (1981) Gastric dilatation associated with acute necrotizing pancreatitis. Eq Pract 3:8–15
Lohman KL, Allen AL (2015) Chronic active interstitial pancreatitis as a cause of transverse colonic obstruction and colic in a horse. Can Vet J 56:1177–1180
Lorenzo-Figueras M, Morisset S, Morisset J et al (2007) Digestive enzyme concentrations and activities in healthy pancreatic tissue of horses. Am J Vet Res 68:1070–1072CrossRef
Martin V, McCutcheon LJ, Poon L et al (1993) Comparative mammal model of chronic rate overload: relationship of myocardial Ca-cycling to heart, metabolic and lipoperoxidation rates. Comp Biochem Physiol B: Comp Biochem 106:453–461CrossRef
McCutcheon LJ, Cory CR, Nowack L et al (1992) Respiratory chain defect of myocardial mitochondria in idiopathic dilated cardiomyopathy of Doberman pinscher dogs. Can J Physiol Pharmacol 70:1529–1533CrossRef
Moon S, Lee JW, Shin D, Shin KY, Kim J, Choi IY, Kim J, Kim H (2015) A genome-wide scan for selective sweeps in racing horses. Asian Australas J Anim Sci 28:1525–1531CrossRef
Newman SJ (2015a) Equine pancreatic disease: a review and characterization of the lesions of 4 cases (2005-2014). J Vet Diagn Investig 27:92–96CrossRef
Newman SJ (2015b) Equine pancreatic disease: a review and characterization of the lesions of four cases (2005–2014). J Vet Diagn Investig 27:92–96CrossRef
O’Brien PJ, Nolan S (2012) Preliminary evaluation of DGGR-substrate, enzymatic lipase assays for sensitivity and specificity for pancreatitis. Vet Clin Pathol 41:E1–E16CrossRef
O’Brien PJ, Landt Y, Ladenson JH (1997) Differential reactivity of cardiac and skeletal muscle from various species in a cardiac troponin I immunoassay. Clin Chem 43:2333–2338CrossRef
O’Brien PJ, Papakonstantinou S, McGrath C (2014) Pancreatic, DGGR-lipase, enzymatic assay is a highly sensitive, specific and inexpensive biomarker of pancreatitis in cats and dogs. Vet Clin Pathol 42:E20–E48
O'Brien PJ, Shen H, McCutcheon LJ, O’Grady M, Byrne PJ, Ferguson HW, Mirsalimi MS, Julian RJ, Sargeant JM, Tremblay RR (1992) Rapid, simple and sensitive microassay for skeletal and cardiac muscle myoglobin and hemoglobin: use in various animals indicates functional role of myohemoproteins. Mol Cell Biochem 112:45–52CrossRef
O'Brien PJ, Dameron GW, Beck ML et al (1997) Cardiac troponin T is a sensitive, specific biomarker of cardiac injury in laboratory animals. Comp Med 47:486–495
O'Brien PJ, Slaughter MR, McKinnon R et al (2002) Advantages of glutamate dehydrogenase as a plasma biomarker of acute hepatic injury in rats. Lab Anim 36:313–321CrossRef
Ollivett TL, Divers TH, Cushing T et al (2012) Acute pancreatitis in two five-day-old Appaloosa foals. Equine Vet J 44:96–99CrossRef
Panteghini M, Bonora R, Pagani F (2001) Measurement of pancreatic lipase activity in serum by a kinetic colorimetric assay using a new chromogenic substrate. Ann Clin Biochem 38:365–370CrossRef
Prinzing U, Zielenski R, Schellong L et al (1998) Kinetic colorimetric assay for pancreatic lipase (PL) based on a chromogenic substrate. Clin Chem 44:122
Tietz NW, Shuey FS (1993) Lipase in serum - the elusive enzyme: an overview. Clin Chem 39:746–756CrossRef
Webb AI, Weaver BM (1979) Body composition of the horse. Eq Vet J 11:39–47CrossRef
Yamout SZ, Nieto JE, Anderson J, de Cock HEV, Vapniarsky N, Aleman M (2012) Pathological evidence of pancreatitis in 43 horses (1986–2011). Equine Vet J 44:45–50CrossRef
Zakaria Z, O’Brien PJ, O’Brien MF et al (2015) The DNA-alkylating, anticancer-drug temozolomide protects rats from dose-limiting pancreatic toxicity produced by the SMAC-mimetic, anticancer-drug TL3271. Vet Clin Pathol 44:E15
Metadata
Title
DGGR-lipase for effective diagnosis of pancreatitis in horses
Authors
Jessica P. Johnson
John D. Stack
Charlotte L. McGivney
Michael P. Garrett
Peter J. O’Brien
Publication date
01-12-2019
Publisher
Springer London
Published in
Comparative Clinical Pathology / Issue 6/2019
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-019-02982-3

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