Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Lasers in Medical Science
Published in: Lasers in Medical Science 3/2020

01-04-2020 | Ovariectomy | Original Article

Improvement in viability and mineralization of osteoporotic bone marrow mesenchymal stem cell through combined application of photobiomodulation therapy and oxytocin

Authors: Somaye Fallahnezhad, Vahid Jajarmi, Sarira Shahnavaz, Abdullah Amini, Seyed Kamran Ghoreishi, Mahsa Kazemi, Sufan Chien, Mohammad Bayat

Published in: Lasers in Medical Science | Issue 3/2020

Login to get access

Abstract

The probable positive effects of photobiomodulation therapy (PBMT) and oxytocin (OT) treatments together or alone were evaluated on cell viability along with the changes in the gene expression of Osteocalcin (OC), Osteoprotegerin (OPG), and Runt-related transcription factor 2 (Runx2) levels of sham (healthy)-Bone marrow mesenchymal stem cell(BMMSC) and ovariectomy-induced osteoporosis (OVX)-BMMSC. BMMSC was harvested from healthy and OVX rats and was cultured in osteogenic induction medium (OIM). There were five groups of BMMSCs: (1) sham -BMMSCs; (2) control -OVX-BMMSCs; (3) OT-treated-OVX-BMMSCs; (4) PBMT–treated-OVX-BMMSCs, and (5) OT + PBMT-OVX-BMMSCs. In all 5 groups, BMMSC viability and proliferation as well as gene expression of OC, OPG, and RUNX2 were evaluated. PBMT and PBMT + OT treatments showed a promising effect on the increased viability of OVX-BMMSC (ANOVA test; LSD test, p = 0.01, p = 0.002). The results of gene expression analysis revealed that the sham- BMMSCs responded optimally to OT treatment. It was also found that OVX-BMMSCs responded optimally to PBMT + OT and PBMT treatments at early and middle stages of osteogenic induction process. Nevertheless, they responded optimally to PBMT + OT and OT especially at the late stage of osteogenic induction process. PBMT and PBMT + OT treatments significantly increased viability of OVX-BMMSC in OIM in vitro. Both PBMT and PBMT + OT treatments could promote mineralization of OVX-BMMSC in the culture medium at early and middle stages of osteogenic induction process. Both OT and PBMT + OT treatments could promote mineralization of OVX-BMMSC in vitro at late stages of osteogenic induction process.
Literature
1.
2.
Sözen T, Özışık L, Başaran NÇ (2017) An overview and management of osteoporosis. Eur J Rheumatol 4:46PubMedCrossRef
3.
Bielby R, Jones E, McGonagle D (2007) The role of mesenchymal stem cells in maintenance and repair of bone. Injury. 38:S26–S32PubMedCrossRef
4.
Tan J, Xu X, Tong Z, Yu Q, Lin Y, Kuang W (2015) Decreased osteogenesis of adult mesenchymal stem cells by reactive oxygen species under cyclic stretch: a possible mechanism of age related osteoporosis. Bone Res 3:15003PubMedPubMedCentralCrossRef
5.
Rodríguez JP, Garat S, Gajardo H, Pino AM, Seitz G (1999) Abnormal osteogenesis in osteoporotic patients is reflected by altered mesenchymal stem cells dynamics. J Cell Biochem 75:414–423PubMedCrossRef
6.
Kiernan J, Davies JE, Stanford WL (2017) Concise review: musculoskeletal stem cells to treat age-related osteoporosis. Stem Cells Transl Med 6:1930–1939PubMedPubMedCentralCrossRef
7.
Bonyadi M, Waldman SD, Liu D, Aubin JE, Grynpas MD, Stanford WL (2003) Mesenchymal progenitor self-renewal deficiency leads to age-dependent osteoporosis in Sca-1/Ly-6A null mice. Proc Natl Acad Sci 100:5840–5845PubMedCrossRefPubMedCentral
8.
Pignolo RJ, Suda RK, McMillan EA, Shen J, Lee SH, Choi Y, Wright AC, Johnson FB (2008) Defects in telomere maintenance molecules impair osteoblast differentiation and promote osteoporosis. Aging Cell 7:23–31PubMedCrossRef
9.
Tewari D, Khan MP, Sagar N, China SP, Singh AK, Kheruka SC, Barai S, Tewari MC, Nagar GK, Vishwakarma AL (2015) Ovariectomized rats with established osteopenia have diminished mesenchymal stem cells in the bone marrow and impaired homing, osteoinduction and bone regeneration at the fracture site. Stem Cell Rev Rep 11:309–321PubMedCrossRef
10.
An JH, Park H, Song JA, Ki KH, Yang J-Y, Choi HJ, Cho SW, Kim SW, Kim SY, Yoo JJ (2013) Transplantation of human umbilical cord blood-derived mesenchymal stem cells or their conditioned medium prevents bone loss in ovariectomized nude mice. Tissue Eng A 19:685–696CrossRef
11.
Lindtner RA, Tiaden AN, Genelin K, Ebner HL, Manzl C, Klawitter M, Sitte I, von Rechenberg B, Blauth M, Richards PJ (2014) Osteoanabolic effect of alendronate and zoledronate on bone marrow stromal cells (BMSCs) isolated from aged female osteoporotic patients and its implications for their mode of action in the treatment of age-related bone loss. Osteoporos Int 25:1151–1161PubMedCrossRef
12.
Nakamura A, Dohi Y, Akahane M, Ohgushi H, Nakajima H, Funaoka H, Takakura Y (2009) Osteocalcin secretion as an early marker of in vitro osteogenic differentiation of rat mesenchymal stem cells. Tissue Eng Part C Methods 15:169–180PubMedCrossRef
13.
14.
Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G (1997) Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 89:747–754PubMedCrossRef
15.
Khalid O, Baniwal SK, Purcell DJ, Leclerc N, Gabet Y, Stallcup MR, Coetzee GA, Frenkel B (2008) Modulation of Runx2 activity by estrogen receptor-alpha: implications for osteoporosis and breast cancer. Endocrinology. 149:5984–5995PubMedPubMedCentralCrossRef
16.
Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao YH, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T (1997) Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell. 89:755–764PubMedCrossRef
17.
Salingcarnboriboon R, Tsuji K, Komori T, Nakashima K, Ezura Y, Noda M (2006) Runx2 is a target of mechanical unloading to alter osteoblastic activity and bone formation in vivo. Endocrinology. 147:2296–2305PubMedCrossRef
18.
19.
de Freitas LF, Hamblin MR (2016) Proposed mechanisms of photobiomodulation or low-level light therapy. IEEE J Sel Top Quantum Electron:22
20.
Wang X, Tian F, Soni SS, Gonzalez-Lima F, Liu H (2016) Interplay between up-regulation of cytochrome-c-oxidase and hemoglobin oxygenation induced by near-infrared laser. Sci Rep 6:30540PubMedPubMedCentralCrossRef
21.
Wang X, Tian F, Reddy DD, Nalawade SS, Barrett DW, Gonzalez-Lima F, Liu H (2017) Up-regulation of cerebral cytochrome-c-oxidase and hemodynamics by transcranial infrared laser stimulation: a broadband near-infrared spectroscopy study. J Cereb Blood Flow Metab 37:3789–3802PubMedPubMedCentralCrossRef
22.
Fallahnezhad S, Amini A, Hajihossainlou B, Chien S, Dadras S, Rezaei F, Bayat M (2018) Combined effects of photobiomodulation and alendronate on viability of osteoporotic bone marrow-derived mesenchymal stem cells. J Photochem Photobiol B Biol 182:77–84CrossRef
23.
Fallahnezhad S, Piryaei A, Tabeie F, Nazarian H, Darbandi H, Amini A, Mostafavinia A, Ghorishi SK, Jalalifirouzkouhi A, Bayat M (2016) Low-level laser therapy with helium–neon laser improved viability of osteoporotic bone marrow-derived mesenchymal stem cells from ovariectomy-induced osteoporotic rats. J Biomed Opt 21:098002CrossRef
24.
Fallahnezhad S, Piryaei A, Darbandi H, Amini A, Ghoreishi SK, Jalalifirouzkouhi R, Bayat M (2018) Effect of low-level laser therapy and oxytocin on osteoporotic bone marrow-derived mesenchymal stem cells. J Cell Biochem 119:983–997PubMedCrossRef
25.
Mostafavinia A, Dehdehi L, Ghoreishi SK, Hajihossainlou B, Bayat M (2017) Effect of in vivo low-level laser therapy on bone marrow-derived mesenchymal stem cells in ovariectomy-induced osteoporosis of rats. J Photochem Photobiol B Biol 175:29–36CrossRef
26.
Fridoni M, Farahani RM, Nejati H, Salimi M, Gharavi SM, Bayat M, Amini A, Torkman G, Bayat S (2015) Evaluation of the effects of LLLT on biomechanical properties of tibial diaphysis in two rat models of experimental osteoporosis by a three point bending test. Lasers Med Sci 30:1117–1125PubMedCrossRef
27.
Mohsenifar Z, Fridoni M, Ghatrehsamani M, Abdollahifar M-A, Abbaszadeh H, Mostafavinia A, Fallahnezhad S, Asghari M, Bayat S, Bayat M (2016) Evaluation of the effects of pulsed wave LLLT on tibial diaphysis in two rat models of experimental osteoporosis, as examined by stereological and real-time PCR gene expression analyses. Lasers Med Sci 31:721–732PubMedCrossRef
28.
Elabd C, Basillais A, Beaupied H, Breuil V, Wagner N, Scheideler M, Zaragosi LE, Massiéra F, Lemichez E, Trajanoski Z (2008) Oxytocin controls differentiation of human mesenchymal stem cells and reverses osteoporosis. Stem Cells 26:2399–2407PubMedCrossRef
29.
Breuil V, Panaia-Ferrari P, Fontas E, Roux C, Kolta S, Eastell R, Ben Yahia H, Faure S, Gossiel F, Benhamou C-L (2014) Oxytocin, a new determinant of bone mineral density in post-menopausal women: analysis of the OPUS cohort. J Clin Endocrinol Metab 99:E634–E641PubMedCrossRef
30.
Beranger GE, Djedaini M, Battaglia S, Roux CH, Scheideler M, Heymann D, Amri E-Z, Pisani DF (2015) Oxytocin reverses osteoporosis in a sex-dependent manner. Front Endocrinol 6:81CrossRef
31.
Wang Z, Goh J, De SD, Ge Z, Ouyang H, Chong JSW, Low SL, Lee EH (2006) Efficacy of bone marrow–derived stem cells in strengthening osteoporotic bone in a rabbit model. Tissue Eng 12:1753–1761PubMedCrossRef
32.
Griffin MD, Ritter T, Mahon BP (2010) Immunological aspects of allogeneic mesenchymal stem cell therapies. Hum Gene Ther 21:1641–1655PubMedCrossRef
33.
Prall WC, Haasters F, Heggebö J, Polzer H, Schwarz C, Gassner C, Grote S, Anz D, Jäger M, Mutschler W (2013) Mesenchymal stem cells from osteoporotic patients feature impaired signal transduction but sustained osteoinduction in response to BMP-2 stimulation. Biochem Biophys Res Commun 440:617–622PubMedCrossRef
34.
Shen J, James AW, Zara JN, Asatrian G, Khadarian K, Zhang JB, Ho S, Kim HJ, Ting K, Soo C (2013) BMP2-induced inflammation can be suppressed by the osteoinductive growth factor NELL-1. Tissue Eng A 19:2390–2401CrossRef
35.
Saeed M, Sattari M, Yeganeh F, Shahnavaz S (2015) Cor-relation between gingival expression of STAT1 and chronic peri-odontitis. Int J Dent Oral Sci 2:158–162
36.
Wu JY, Wang YH, Wang GJ, Ho ML, Wang CZ, Yeh ML, Chen CH (2012) Low-power GaAlAs laser irradiation promotes the proliferation and osteogenic differentiation of stem cells via IGF1 and BMP2. PLoS One 7:e44027PubMedPubMedCentralCrossRef
37.
Baron W, Metz B, Bansal R, Hoekstra D, de Vries H (2000) PDGF and FGF-2 signaling in oligodendrocyte progenitor cells: regulation of proliferation and differentiation by multiple intracellular signaling pathways. Mol Cell Neurosci 15:314–329PubMedCrossRef
38.
Chen W, Baylink DJ, Brier-Jones J, Neises A, Kiroyan JB, Rundle CH, Lau KH, Zhang XB (2015) PDGFB-based stem cell gene therapy increases bone strength in the mouse. Proc Natl Acad Sci U S A 112:E3893–E3900PubMedPubMedCentralCrossRef
39.
40.
McLaughlin PJ, Cain JD, Titunick MB, Sassani JW, Zagon IS (2017) Topical naltrexone is a safe and effective alternative to standard treatment of diabetic wounds. Adv Wound Care 6:279–288CrossRef
41.
42.
Noiseux N, Borie M, Desnoyers A, Menaouar A, Stevens LM, Mansour S, Danalache BA, Roy DC, Jankowski M, Gutkowska J (2012) Preconditioning of stem cells by oxytocin to improve their therapeutic potential. Endocrinology. 153:5361–5372PubMedCrossRef
43.
Kim YS, Ahn Y, Kwon JS, Cho YK, Jeong MH, Cho JG, Park JC, Kang JC (2012) Priming of mesenchymal stem cells with oxytocin enhances the cardiac repair in ischemia/reperfusion injury. Cells Tissues Organs 195:428–442PubMedCrossRef
44.
Zhang H, Li H (2018) Tricin enhances osteoblastogenesis through the regulation of Wnt/beta-catenin signaling in human mesenchymal stem cells. Mech Dev 152:38–43PubMedCrossRef
45.
Liu TM, Lee EH (2013) Transcriptional regulatory cascades in Runx2-dependent bone development. Tissue Eng B Rev 19:254–263CrossRef
46.
Otto F, Thornell AP, Crompton T, Denzel A, Gilmour KC, Rosewell IR, Stamp GW, Beddington RS, Mundlos S, Olsen BR, Selby PB, Owen MJ (1997) Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell. 89:765–771PubMedCrossRef
47.
Rocha Júnior AM, Vieira BJ, Andrade LCF d, Aarestrup FM (2007) Effects of low-level laser therapy on the progress of wound healing in humans: the contribution of in vitro and in vivo experimental studies. J Vasc Bras 6:257–265CrossRef
48.
Lev-Sagie A, Kopitman A, Brzezinski A (2017) Low-level laser therapy for the treatment of provoked vestibulodynia—a randomized, placebo-controlled pilot trial. J Sex Med 14:1403–1411PubMedCrossRef
49.
Arunachalam LT, Sudhakar U, Janarthanam AS, Das NM (2014) Effect of low level laser therapy on revascularization of free gingival graft using ultrasound Doppler flowmetry. J Indian Soc Periodontol 18:403PubMedPubMedCentralCrossRef
50.
Cassano P, Tran AP, Katnani H, Bleier BS, Hamblin MR, Yuan Y, Fang Q (2019) Selective photobiomodulation for emotion regulation: model-based dosimetry study. Neurophotonics. 6:015004PubMedPubMedCentralCrossRef
Metadata
Title
Improvement in viability and mineralization of osteoporotic bone marrow mesenchymal stem cell through combined application of photobiomodulation therapy and oxytocin
Authors
Somaye Fallahnezhad
Vahid Jajarmi
Sarira Shahnavaz
Abdullah Amini
Seyed Kamran Ghoreishi
Mahsa Kazemi
Sufan Chien
Mohammad Bayat
Publication date
01-04-2020
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 3/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-019-02848-8

Other articles of this Issue 3/2020

Lasers in Medical Science 3/2020 Go to the issue

Review Article

Effects of low level laser therapy in cancer cells—a systematic review of the literature

Original Article

Is laser-assisted resection preferable to lobectomy for pulmonary metastasectomy?

Original Article

Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells

Correction

Correction to: Photobiomodulation by dual-wavelength low-power laser effects on infected pressure ulcers

Original Article

Low-level laser therapy for weight reduction: a randomized pilot study

Original Article

In vitro anti-tumor effect of low-power laser irradiation (LPLI) on gastroenterological carcinoma cells