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Calcified Tissue International
Published in: Calcified Tissue International 6/2010

01-12-2010

Osteogenic Potential of Biosilica on Human Osteoblast-Like (SaOS-2) Cells

Authors: Matthias Wiens, Xiaohong Wang, Ute Schloßmacher, Ingo Lieberwirth, Gunnar Glasser, Hiroshi Ushijima, Heinz C. Schröder, Werner E. G. Müller

Published in: Calcified Tissue International | Issue 6/2010

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Abstract

Biosilica is a natural polymer, synthesized by the poriferan enzyme silicatein from monomeric silicate substrates. Biosilica stimulates mineralizing activity and gene expression of SaOS-2 cells. To study its effect on the formation of hydroxyapatite (HA), SaOS-2 cells were grown on different silicatein/biosilica-modified substrates (bone slices, Ca–P-coated coverslips, glass coverslips). Growth on these substrates induced the formation of HA nodules, organized in longitudinal arrays or spherical spots. Nodules of sizes above 1 μm were composed of irregularly arranged HA prism-like nanorods, formed by aggregates of three to eight SaOS-2 cells. Moreover, growth on silicatein/biosilica-modified substrates elicited increased [3H]dT incorporation into DNA, indicative of enhanced cell proliferation. Consequently, an in vitro-based bioassay was established to determine the ratio between [3H]dT incorporation and HA formation. This ratio was significantly higher for cells that grew on silicatein/biosilica-modified substrates than for cells on Ca–P-coated coverslips or plain glass slips. Hence, we propose that this ratio of in vitro-determined parameters reflects the osteogenic effect of different substrates on bone-forming cells. Finally, qRT-PCR analyses demonstrated that growth of SaOS-2 cells on a silicatein/biosilica matrix upregulated BMP2 (bone morphogenetic protein 2, inducer of bone formation) expression. In contrast, TRAP (tartrate-resistant acid phosphatase, modulator of bone resorption) expression remained unaffected. We conclude that biosilica shows pronounced osteogenicity in vitro, qualifying this material for studies of bone replacement also in vivo.
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Metadata
Title
Osteogenic Potential of Biosilica on Human Osteoblast-Like (SaOS-2) Cells
Authors
Matthias Wiens
Xiaohong Wang
Ute Schloßmacher
Ingo Lieberwirth
Gunnar Glasser
Hiroshi Ushijima
Heinz C. Schröder
Werner E. G. Müller
Publication date
01-12-2010
Publisher
Springer-Verlag
Published in
Calcified Tissue International / Issue 6/2010
Print ISSN: 0171-967X
Electronic ISSN: 1432-0827
DOI
https://doi.org/10.1007/s00223-010-9408-6

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