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BMC Complementary Medicine and Therapies

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Open Access 01-12-2021 | Osteoarthrosis | Research article

Up-regulation of long non-coding RNA CYTOR induced by icariin promotes the viability and inhibits the apoptosis of chondrocytes

Authors: Guoyou Wang, Lei Zhang, Huarui Shen, Qi Hao, Shijie Fu, Xia Liu

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

Abstract

Background

Icariin (ICAR) is the main effective component extracted from epimedium, and is reported to have the potential to treat osteoarthritis (OA). However, its pharmacological function on chondrocytes has not been fully clarified.

Methods

Different doses of ICAR were used to treat chondrocyte cell lines, including CHON-001 and ATDC5. Then the expressions of different lncRNAs were measured by qRT-PCR. Interleukin-1β (IL-1β) was used to simulate the inflammatory response environment of chondrocytes. Overexpression plasmids and short hairpin RNAs of lncRNA CYTOR were used to construct gain-of-function and loss of function models. CCK-8 was conducted to determine the cell viability. Flow cytometry was used to detect the apoptosis of chondrocytes. Enzyme-linked immunosorbent assay (ELISA) was adopted to measure the contents of inflammatory factors (IL-6, IL-8, TNF-α) in the supernatant of the chondrocytes.

Results

Compared with other lncRNAs, CYTOR was changed most significantly in both CHON-001 and ATDC5 cells after treatment with ICAR. ICAR promotes the viability and inhibits the apoptosis of CHON-001 and ATDC5 cells induced by IL-1β, accompanied with reduced levels of inflammatory factors. Overexpression of CYTOR facilitated the viability of chondrocytes, while repressed their apoptosis and inflammatory response. What’s more, knockdown of CYTOR reversed the protective effects of ICAR on chondrocytes.

Conclusion

CYTOR was a pivotal lncRNA involved in the protective function of ICAR on chondrocytes.

Glyn-Jones S, Palmer AJ, Agricola R, Price AJ, Vincent TL, Weinans H, et al. Osteoarthritis. Lancet. 2015;386(9991):376–87. https://doi.org/10.1016/S0140-6736(14)60802-3.CrossRefPubMed

Zhang Y, Jordan JM. Epidemiology of osteoarthritis. Clin Geriatr Med. 2010;26(3):355–69. https://doi.org/10.1016/j.cger.2010.03.001.CrossRefPubMedPubMedCentral

Sinusas K. Osteoarthritis: diagnosis and treatment. Am Fam Physician. 2012;85:49–56.PubMed

Bijlsma JW, Berenbaum F, Lafeber FP. Osteoarthritis: an update with relevance for clinical practice. LANCET. 2011;377(9783):2115–26. https://doi.org/10.1016/S0140-6736(11)60243-2.CrossRefPubMed

Zhou J, Wu J, Chen X, Fortenbery N, Eksioglu E, Kodumudi KN, et al. Icariin and its derivative, ICT, exert anti-inflammatory, anti-tumor effects, and modulate myeloid derived suppressive cells (MDSCs) functions. Int Immunopharmacol. 2011;11(7):890–8. https://doi.org/10.1016/j.intimp.2011.01.007.CrossRefPubMed

Ming LG, Chen KM, Xian CJ. Functions and action mechanisms of flavonoids genistein and icariin in regulating bone remodeling. J Cell Physiol. 2013;228(3):513–21. https://doi.org/10.1002/jcp.24158.CrossRefPubMed

Zu Y, Mu Y, Li Q, Zhang ST, Yan HJ. Icariin alleviates osteoarthritis by inhibiting NLRP3-mediated pyroptosis. J Orthop Surg Res. 2019;14(1):307. https://doi.org/10.1186/s13018-019-1307-6.CrossRefPubMedPubMedCentral

Fatica A, Bozzoni I. Long non-coding RNAs: new players in cell differentiation and development. Nat Rev Genet. 2014;15(1):7–21. https://doi.org/10.1038/nrg3606.CrossRef

Hu P, Sun F, Ran J, Wu L. Identify CRNDE and LINC00152 as the key lncRNAs in age-related degeneration of articular cartilage through comprehensive and integrative analysis. PEERJ. 2019;7:e7024. https://doi.org/10.7717/peerj.7024.CrossRefPubMedPubMedCentral

10.

Saxby DJ, Lloyd DG. Osteoarthritis year in review 2016: mechanics. Osteoarthr Cartil. 2017;25(2):190–8. https://doi.org/10.1016/j.joca.2016.09.023.CrossRef

11.

Lei J, Fu Y, Zhuang Y, Zhang K, Lu D. LncRNA SNHG1 alleviates IL-1beta-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-kappaB signaling pathways. Biosci Rep. 2019;39(9). https://doi.org/10.1042/BSR20191523.

12.

Shen R, Wang JH. The effect of icariin on immunity and its potential application. Am J Clin Exp Immunol. 2018;7:50–6.PubMedPubMedCentral

13.

Nian H, Ma MH, Nian SS, Xu LL. Antiosteoporotic activity of icariin in ovariectomized rats. Phytomedicine. 2009;16(4):320–6. https://doi.org/10.1016/j.phymed.2008.12.006.CrossRefPubMed

14.

Ai D, Yu F. LncRNA DNM3OS promotes proliferation and inhibits apoptosis through modulating IGF1 expression by sponging MiR-126 in CHON-001 cells. Diagn Pathol. 2019;14(1):106. https://doi.org/10.1186/s13000-019-0877-2.CrossRefPubMedPubMedCentral

15.

Liang J, Wei X, Liu Z, Cao D, Tang Y, Zou Z, et al. Long noncoding RNA CYTOR in cancer: a TCGA data review. Clin Chim Acta. 2018;483:227–33. https://doi.org/10.1016/j.cca.2018.05.010.CrossRefPubMed

16.

Yue B, Liu C, Sun H, Liu M, Song C, Cui R, et al. A positive feed-forward loop between LncRNA-CYTOR and Wnt/beta-catenin signaling promotes metastasis of Colon Cancer. Mol Ther. 2018;26(5):1287–98. https://doi.org/10.1016/j.ymthe.2018.02.024.CrossRefPubMedPubMedCentral

17.

Zheng X, Dong S, Sun L, Xu J, Liu J, Hao R. LncRNA LINC00152 promotes laryngeal Cancer progression by sponging MiR-613. Open Med (Wars). 2020;15(1):240–8. https://doi.org/10.1515/med-2020-0035.CrossRef

18.

Zhu H, Shan Y, Ge K, Lu J, Kong W, Jia C. LncRNA CYTOR promotes pancreatic cancer cell proliferation and migration by sponging miR-205-5p. Pancreatology. 2020;20(6):1139–48. https://doi.org/10.1016/j.pan.2020.05.004.CrossRefPubMed

19.

Adli M, Merkhofer E, Cogswell P, Baldwin AS. IKKalpha and IKKbeta each function to regulate NF-kappaB activation in the TNF-induced/canonical pathway. PLoS One. 2010;5(2):e9428. https://doi.org/10.1371/journal.pone.0009428.CrossRefPubMedPubMedCentral

20.

Radons J, Bosserhoff AK, Grassel S, Falk W, Schubert TE. P38MAPK mediates IL-1-induced down-regulation of aggrecan gene expression in human chondrocytes. Int J Mol Med. 2006;17:661–8.PubMed

Title: Up-regulation of long non-coding RNA CYTOR induced by icariin promotes the viability and inhibits the apoptosis of chondrocytes
Authors: Guoyou Wang
Lei Zhang
Huarui Shen
Qi Hao
Shijie Fu
Xia Liu
Publication date: 01-12-2021
Publisher: BioMed Central
Keywords: Osteoarthrosis
Osteoarthrosis
Published in: BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-021-03322-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Up-regulation of long non-coding RNA CYTOR induced by icariin promotes the viability and inhibits the apoptosis of chondrocytes

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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