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Langenbeck's Archives of Surgery

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01-05-2008 | Original Article

Optimising post-conditioning time of marginal donor livers

Authors: Steffen Manekeller, Alexandra Seinsche, Judith Stegemann, Andreas Hirner

Published in: Langenbeck's Archives of Surgery | Issue 3/2008

Abstract

Background

Due to the discrepancy between organ donors and receptors, the use of marginal livers (e.g., non-heart-beating-donor grafts) for transplantation purpose increased. The potential of a short-term aerobic machine perfusion (post-conditioning) for “less than optimal” grafts after cold storage (CS) was recently demonstrated. In our study, the optimal time course of post-conditioning (PC) is to be evaluated.

Materials and methods

Livers from male Wistar rats were withdrawn 30 min after cardiac arrest and flushed with histidine tryptophan ketoglutarate (HTK) solution. Then they were stored in HTK at 4°C for 18 h. After 16 h, some livers were put on PC by cold perfusion with HTK for 0.5, 1, 2 or 3 h. Afterwards, the viability of the organs was estimated by warm reperfusion (2 h) in vitro.

Results

After 1 h of PC, a significant increase in bile production and a decrease in enzyme release could be detected in comparison to CS. The adenosine triphosphate content of the PC livers after 1 h of treatment was significant higher than in CS organs. No markers for apoptosis could be detected after 1 h PC.

Conclusion

It can be concluded that a PC of 1 h after cold storage can ameliorate the organ viability of marginal livers. The extension or abbreviation of PC time seems to have no further beneficial effects.

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Title: Optimising post-conditioning time of marginal donor livers
Authors: Steffen Manekeller
Alexandra Seinsche
Judith Stegemann
Andreas Hirner
Publication date: 01-05-2008
Publisher: Springer-Verlag
Published in: Langenbeck's Archives of Surgery / Issue 3/2008
Print ISSN: 1435-2443
Electronic ISSN: 1435-2451
DOI: https://doi.org/10.1007/s00423-008-0288-4

At a glance: The STEP trials

Springer Medicine

Optimising post-conditioning time of marginal donor livers

Abstract

Background

Materials and methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Materials and methods

Results

Conclusion

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