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Obesity Surgery
Published in: Obesity Surgery 6/2020

01-06-2020 | Obesity | Original Contributions

Two Bariatric Surgical Procedures Differentially Alter the Intestinal Microbiota in Obesity Patients

Authors: Guolin Chen, Jingshen Zhuang, Qianwen Cui, Shuwen Jiang, Weihua Tao, Wanqun Chen, Shuqing Yu, Lina Wu, Wah Yang, Fucheng Liu, Jingge Yang, Cunchuan Wang, Shiqi Jia

Published in: Obesity Surgery | Issue 6/2020

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Abstract

Aims

To explore the intestinal microbiota composition affected by the two most widely used procedures of bariatric surgery, laparoscopic sleeve gastrectomy (LSG) and laparoscopic roux-en-Y gastric bypass (LRYGB), in Chinese obesity patients.

Methods

Stool samples were collected from the obese patients before (n = 87) and with follow-up after the surgery (n = 53). After DNA extraction, 16S rDNA (V3 + V4 regions) sequencing was completed on Illumina HiSeq 2500 sequencing platform. The samples were analyzed base on four groups, pre-LSG (n = 54), pre-LRYGB (n = 33), post-LSG (n = 33), and post-LRYGB (n = 20). The linear mixed models were used to analyze the alteration of intestinal microbiota before and after the surgeries of LSG or LRYGB. Student’s t test and χ2 test were used for analysis of independent groups; Metastats analysis was used to compare the relative abundance of bacteria, and Pearson correlation and Spearman correlation analysis were used to test the correlation between indicated groups.

Results

87 patients were included and 53 (60.92%) of them completed the follow-up (9.60 ± 3.92 months). Body mass index (BMI) decreased from 37.84 ± 6.16 kg/m2 to 26.22 ± 4.33 kg/m2 after LSG and from 45.75 ± 14.26 kg/m2 to 33.15 ± 10.99 kg/m2 after LRYGB. The relative abundance of 5 phyla and 42 genera were altered after the surgery in the cohort. Although no alteration of Firmicutes was observed at phylum level, 54.76% of the altered genera belong to phylum Firmicutes. Both LSG and LRYGB procedures increased the richness and evenness of intestinal microbiota in obese patients after the surgery. Particularly, 33 genera altered after LSG and 19 genera altered after LRYGB, in which 11 genera were common alterations in both procedures.

Conclusion

Both LSG and LRYGB altered the composition of intestinal microbiota in Chinese obesity patients, and particularly increased the richness and evenness of microbiota. Genera belonging to phylum Firmicutes were the most altered bacteria by bariatric surgery. The procedure of LSG resulted in much more pronounced alteration of the intestinal microbiota abundance than that observed in LRYGB. While different genera were altered after LSG and LRYGB procedures, 10 genera were the common altered genera in both procedures. Bacteria altered after LSG and LRYGB were functionally associated with BMI, and with relieving of the metabolic syndromes.
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Metadata
Title
Two Bariatric Surgical Procedures Differentially Alter the Intestinal Microbiota in Obesity Patients
Authors
Guolin Chen
Jingshen Zhuang
Qianwen Cui
Shuwen Jiang
Weihua Tao
Wanqun Chen
Shuqing Yu
Lina Wu
Wah Yang
Fucheng Liu
Jingge Yang
Cunchuan Wang
Shiqi Jia
Publication date
01-06-2020
Publisher
Springer US
Published in
Obesity Surgery / Issue 6/2020
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-020-04494-4

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