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01-02-2017 | Original Article

Novel glycoside from Wedelia calendulacea inhibits diethyl nitrosamine-induced renal cancer via downregulating the COX-2 and PEG₂ through nuclear factor-κB pathway

Authors: Amita Verma, Bahar Ahmed, Firoz Anwar, Mahfoozur Rahman, Dinesh Kumar Patel, Gaurav Kaithwas, Ravi Rani, Prakash C Bhatt, Vikas Kumar

Published in: Inflammopharmacology | Issue 1/2017

Abstract

A new compound derivative of glycoside 19-α-hydroxy-ursolic acid glucoside (19-α-hydroxyurs-12(13)-ene-28-oic acid-3-O-β-d-glucopyranoside (HEG) was isolated from whole plant of Wedelia calendulacea (Compositae). The structure was elucidated and established by standard spectroscopy approaches. Diethylnitrosamine (DEN) (200 mg/kg) and ferric nitrilotriacetate (Fe-NTA) (9 mg/kg) were used for induction of renal cell carcinoma (RCC) in the rats. The rats were further divided into different groups and were treated with HEG doses for 22 weeks. Anti-cancer effect in RCC by HEG was dose dependent to restrict the macroscopical changes as compared to DEN + Fe-NTA-control animals. Significant alteration in biochemical parameters and dose-dependent alleviation in Phase I and Phase II antioxidant enzymes were responsible for its chemo-protective nature. HEG in dose-dependent manner was significant to alter the elevated levels of pro-inflammatory cytokines and inflammatory mediators during RCC. The histopathological changes were observed in the HEG pre-treated group, which was proof for its safety concern as far as its toxicity is concerned. The isolated compound HEG can impart momentous chemo-protection against experimental RCC by suppressing the cyclooxygenase (COX-2) and prostaglandin E2 (PGE₂) expression via nuclear factor-kappa B (NF-κB) pathway.

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Title: Novel glycoside from Wedelia calendulacea inhibits diethyl nitrosamine-induced renal cancer via downregulating the COX-2 and PEG2 through nuclear factor-κB pathway
Authors: Amita Verma
Bahar Ahmed
Firoz Anwar
Mahfoozur Rahman
Dinesh Kumar Patel
Gaurav Kaithwas
Ravi Rani
Prakash C Bhatt
Vikas Kumar
Publication date: 01-02-2017
Publisher: Springer International Publishing
Published in: Inflammopharmacology / Issue 1/2017
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0310-y

At a glance: The STEP trials

Springer Medicine

Novel glycoside from Wedelia calendulacea inhibits diethyl nitrosamine-induced renal cancer via downregulating the COX-2 and PEG₂ through nuclear factor-κB pathway

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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