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Graefe's Archive for Clinical and Experimental Ophthalmology

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01-01-2012 | Basic Science

Nicotine reduces VEGF-secretion and phagocytotic activity in porcine RPE

Authors: Alexa Karina Klettner, Johanna Doths, Johann Roider

Published in: Graefe's Archive for Clinical and Experimental Ophthalmology | Issue 1/2012

Abstract

Background/Purpose

Smoking is a strong environmental factor for the development of age-related macula degeneration. In this study, we investigated the effects of nicotine on RPE cell function in porcine in vitro models, focussing on cell death, VEGF secretion and phagocytotic ability.

Methods

For these experiments, perfusion organ culture and primary RPE cell culture were used and exposed to nicotine up to 7 days. Survival was investigated in primary porcine RPE cells in an MTT and trypan blue exclusion assay. VEGF secretion was investigated in a porcine perfusion organ culture model using ELISA. A phagocytosis assay using FITC-labelled latex beads in primary RPE cells was used to assess the phagocytotic ability of the cells.

Results

Nicotine does not induce cell death in the RPE at any time point up to 7 days of stimulation at any tested concentration. VEGF secretion, however, is diminished compared to untreated control already after 1 day of nicotine treatment and even more profoundly up to 7 days. Furthermore, phagocytotic ability of the RPE is diminished by nicotine in the highest concentrations tested (100 μM).

Conclusion

Nicotine impedes RPE function (VEGF secretion, phagocytosis), which could be directly involved in the development of dry AMD and geographic atrophy.

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Title: Nicotine reduces VEGF-secretion and phagocytotic activity in porcine RPE
Authors: Alexa Karina Klettner
Johanna Doths
Johann Roider
Publication date: 01-01-2012
Publisher: Springer-Verlag
Published in: Graefe's Archive for Clinical and Experimental Ophthalmology / Issue 1/2012
Print ISSN: 0721-832X
Electronic ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-011-1776-8

At a glance: The STEP trials

Springer Medicine

Nicotine reduces VEGF-secretion and phagocytotic activity in porcine RPE

Abstract

Background/Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background/Purpose

Methods

Results

Conclusion

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