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Archives of Orthopaedic and Trauma Surgery
Published in: Archives of Orthopaedic and Trauma Surgery 7/2010

01-07-2010 | Basic Science

NgR expression in macrophages promotes nerve regeneration after spinal cord injury in rats

Authors: Zhuangchen Zhu, Bin Ni, Guodong Yin, Fengjin Zhou, Jun Liu, Qunfeng Guo, Xiang Guo

Published in: Archives of Orthopaedic and Trauma Surgery | Issue 7/2010

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Abstract

Objective

This study aimed to investigate the expression of Nogo-66 receptor (NgR) in macrophages after SCI and clarify its role in neuron regeneration.

Methods

Macrophages harvested from injured spine cord of rats were stained by double immunofluorescence labeling technique to observe the expression of NgR at histological and cellular levels. Macrophages which expressed NgR were constructed in vitro, and then the effects of NgR on macrophage phagocytosis and neuraxon regeneration in three groups (NgR-macrophages group, mock group and normal macrophages group) were studied using Western blot, micro-MTT colorimetry, and LDH assay separately.

Results

The results showed that CD68-positive macrophages in injured tissue of spine cord expressed NgR after double immunofluorescence staining on day 7 after SCI, and so did macrophages isolated and cultured from the injured spine cord. The results of Western blot showed that phagocytosis of macrophages in NgR-macrophages group was much better than that in mock group and normal macrophage group (p < 0.05). And the results of Micro-MTT colorimetry and LDH assay indicated that the capacity of neuraxon regeneration in NgR-macrophages group was significantly higher than that in the other two groups (p < 0.05).

Conclusions

The results suggested that there was NgR expressing in the infiltrated macrophages following SCI, which increased phagocytosis of the macrophages, and promoted post-SCI CNS regeneration in vitro.
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Metadata
Title
NgR expression in macrophages promotes nerve regeneration after spinal cord injury in rats
Authors
Zhuangchen Zhu
Bin Ni
Guodong Yin
Fengjin Zhou
Jun Liu
Qunfeng Guo
Xiang Guo
Publication date
01-07-2010
Publisher
Springer-Verlag
Published in
Archives of Orthopaedic and Trauma Surgery / Issue 7/2010
Print ISSN: 0936-8051
Electronic ISSN: 1434-3916
DOI
https://doi.org/10.1007/s00402-010-1065-8

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