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Open Access 01-12-2019 | Research

Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice

Authors: Hareram Birla, Chetan Keswani, Sachchida Nand Rai, Saumitra Sen Singh, Walia Zahra, Hagera Dilnashin, Aaina Singh Rathore, Surya Pratap Singh

Published in: Behavioral and Brain Functions | Issue 1/2019

Abstract

Background

Bisphenol A (BPA), a major endocrine disruptor and a xenobiotic compound is used abundantly in the production of polycarbonate plastics and epoxy resins. Human exposure to this compound is primarily via its leaching from the protective internal epoxy resin coatings of containers into the food and beverages. In addition, the plastics used in dental prostheses and sealants also contain considerable amount of BPA and have a high risk of human exposure. Since it is a well-known endocrine disruptor and closely mimics the molecular structure of human estrogen thereby impairing learning and memory. Withania somnifera (Ws), commonly known as Ashwagandha is known for its varied therapeutic uses in Ayurvedic system of medicine. The present study was undertaken to demonstrate the impairment induced by BPA on the spatial learning, working memory and its alleviation by Ws in Swiss albino mice. The study was conducted on thirty Swiss albino mice, randomly distributed among three groups: control, BPA and BPA + Ws. The behavioral recovery after treatment with Ws was investigated using the Y-maize and Morris water maize test. Whereas, for the estimation of recovery of NMDA receptor which is related to learning and memory in hippocampus region by western blot and immunohistochemistry. Furthermore, the oxidative stress and antioxidant level was assessed by biochemical tests like MDA, SOD and catalase.

Results

The study revealed that administration of Ws alleviated the behavioral deficits induced by BPA. Alongside, Ws treatment reinstated the number of NMDA receptors in hippocampus region and showed anti-oxidative property while ameliorating the endogenous anti-oxidant level in the brain.

Conclusion

These findings suggest that Ws significantly ameliorates the level of BPA intoxicated oxidative stress thereby potentially treating cognitive dysfunction which acts as the primary symptom in a number of neurodegenerative diseases.

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Title: Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice
Authors: Hareram Birla
Chetan Keswani
Sachchida Nand Rai
Saumitra Sen Singh
Walia Zahra
Hagera Dilnashin
Aaina Singh Rathore
Surya Pratap Singh
Publication date: 01-12-2019
Publisher: BioMed Central
Published in: Behavioral and Brain Functions / Issue 1/2019
Electronic ISSN: 1744-9081
DOI: https://doi.org/10.1186/s12993-019-0160-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Neuroprotective effects of Withania somnifera in BPA induced-cognitive dysfunction and oxidative stress in mice

Abstract

Background

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Results

Conclusion

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