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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2023

Open Access 01-12-2023 | Neuropathic Pain | Research

TSLP in DRG neurons causes the development of neuropathic pain through T cells

Authors: Yuka Ino, Motoyo Maruyama, Masumi Shimizu, Rimpei Morita, Atsuhiro Sakamoto, Hidenori Suzuki, Atsushi Sakai

Published in: Journal of Neuroinflammation | Issue 1/2023

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Abstract

Background

Peripheral nerve injury to dorsal root ganglion (DRG) neurons develops intractable neuropathic pain via induction of neuroinflammation. However, neuropathic pain is rare in the early life of rodents. Here, we aimed to identify a novel therapeutic target for neuropathic pain in adults by comprehensively analyzing the difference of gene expression changes between infant and adult rats after nerve injury.

Methods

A neuropathic pain model was produced in neonatal and young adult rats by spared nerve injury. Nerve injury-induced gene expression changes in the dorsal root ganglion (DRG) were examined using RNA sequencing. Thymic stromal lymphopoietin (TSLP) and its siRNA were intrathecally injected. T cells were examined using immunofluorescence and were reduced by systemic administration of FTY720.

Results

Differences in changes in the transcriptome in injured DRG between infant and adult rats were most associated with immunological functions. Notably, TSLP was markedly upregulated in DRG neurons in adult rats, but not in infant rats. TSLP caused mechanical allodynia in adult rats, whereas TSLP knockdown suppressed the development of neuropathic pain. TSLP promoted the infiltration of T cells into the injured DRG and organized the expressions of multiple factors that regulate T cells. Accordingly, TSLP caused mechanical allodynia through T cells in the DRG.

Conclusion

This study demonstrated that TSLP is causally involved in the development of neuropathic pain through T cell recruitment.
Appendix
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Metadata
Title
TSLP in DRG neurons causes the development of neuropathic pain through T cells
Authors
Yuka Ino
Motoyo Maruyama
Masumi Shimizu
Rimpei Morita
Atsuhiro Sakamoto
Hidenori Suzuki
Atsushi Sakai
Publication date
01-12-2023
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2023
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/s12974-023-02882-y

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