Published in:
01-05-2011 | Original Article
Myocardial substrate and route of administration determine acute cardiac retention and lung bio-distribution of cardiosphere-derived cells
Authors:
Michael Bonios, MD, John Terrovitis, MD, Connie Y. Chang, MSE, James M. Engles, MS, MBA, Takahiro Higuchi, MD, PhD, Riikka Lautamäki, MD, Jianhua Yu, BS, James Fox, BS, Martin Pomper, MD, PhD, Richard L. Wahl, MD, Benjamin M. Tsui, PhD, Brian O’Rourke, MD, PhD, Frank M. Bengel, MD, Eduardo Marbán, MD, PhD, M. Roselle Abraham, MD
Published in:
Journal of Nuclear Cardiology
|
Issue 3/2011
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Abstract
Background
Quantification of acute myocardial retention and lung bio-distribution of cardiosphere-derived cells (CDCs) following transplantation is important to improve engraftment.
Methods and results
We studied acute(1 hour) cardiac/lung retention in 4 groups (n = 25) of rats (normal—NL, acute ischemia-reperfusion—AI-RM, acute permanent ligation—PL, and chronic infarct by ischemia-reperfusion—CI-R) using intra-myocardial delivery, 1 group using intracoronary delivery (acute ischemia-reperfusion, AI-RC, n = 5) and 1 group using intravenous delivery (acute ischemia-reperfusion, AI-RV, n = 5) of CDCs by PET. Cardiac retention was similar in the NL, AI-RM, CI-R, and A-IRC groups (13.6% ± 2.3% vs 12.0% ± 3.9% vs 9.9 ± 2.8 vs 15.4% ± 5.5%; P = NS), but higher in PL animals (22.9% ± 5.2%; P < .05). Low cardiac retention was associated with significantly higher lung activity in NL and AI-RM groups (43.3% ± 5.6% and 39.9% ± 9.3%), compared to PL (28.5% ± 5.9%), CI-R (20.2% ± 9.3%), and A-IRC (19.9% ± 5.6%) animals (P < .05 vs AI-RM and NL). Lung activity was highest following intravenous CDC delivery (55.1% ± 9.3%, P < .001) and was associated with very low cardiac retention (0.8% ± 1.06%). Two-photon microscopy indicated that CDCs escaped to the lungs via the coronary veins following intra-myocardial injection.
Conclusions
Acute cardiac retention and lung bio-distribution vary with the myocardial substrate and injection route. Intra-myocardially injected CDCs escape into the lungs via coronary veins, an effect that is more pronounced in perfused myocardium.