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Archives of Virology
Published in: Archives of Virology 6/2014

01-06-2014 | Brief Report

Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae)

Authors: Ying Zhai, Sudeep Bag, Neena Mitter, Massimo Turina, Hanu R. Pappu

Published in: Archives of Virology | Issue 6/2014

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Abstract

Tospoviruses cause serious economic losses to a wide range of field and horticultural crops on a global scale. The NSs gene encoded by tospoviruses acts as a suppressor of host plant defense. We identified amino acid motifs that are conserved in all of the NSs proteins of tospoviruses for which the sequence is known. Using tomato spotted wilt virus (TSWV) as a model, the role of these motifs in suppressor activity of NSs was investigated. Using site-directed point mutations in two conserved motifs, glycine, lysine and valine/threonine (GKV/T) at positions 181-183 and tyrosine and leucine (YL) at positions 412-413, and an assay to measure the reversal of gene silencing in Nicotiana benthamiana line 16c, we show that substitutions (K182 to A, and L413 to A) in these motifs abolished suppressor activity of the NSs protein, indicating that these two motifs are essential for the RNAi suppressor function of tospoviruses.
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Metadata
Title
Mutational analysis of two highly conserved motifs in the silencing suppressor encoded by tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae)
Authors
Ying Zhai
Sudeep Bag
Neena Mitter
Massimo Turina
Hanu R. Pappu
Publication date
01-06-2014
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2014
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1928-8

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