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01-11-2014 | Brief Report

Multiplex RT-PCR detection of three common viruses infecting orchids

Authors: Raymond N. Ali, Alison L. Dann, Peter A. Cross, Calum R. Wilson

Published in: Archives of Virology | Issue 11/2014

Abstract

A multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for simultaneous detection of three orchid viruses: cymbidium mosaic virus (CymMV), odontoglossum ringspot virus (ORSV), and orchid fleck virus (OFV). Primers were used to amplify nucleocapsid protein gene fragments of 845 bp (ORSV), 505 bp (CymMV) and 160 bp (OFV). A 60-bp amplicon of plant glyceraldehyde-3-phophate dehydrogenase mRNA was included as an internal control against false negatives. The assay was validated against 31 collected plants from six orchid genera and compared with results obtained by transmission electron microscopy (TEM). The RT-PCR assay proved more sensitive than TEM for detection of OFV.

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Title: Multiplex RT-PCR detection of three common viruses infecting orchids
Authors: Raymond N. Ali
Alison L. Dann
Peter A. Cross
Calum R. Wilson
Publication date: 01-11-2014
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 11/2014
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2161-9

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