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Virology Journal
Published in: Virology Journal 1/2014

Open Access 01-12-2014 | Research

Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia

Authors: Nary Ly, Rafal Tokarz, Nischay Mishra, Stephen Sameroff, Komal Jain, Agus Rachmat, Ung Sam An, Steven Newell, Dustin J Harrison, W Ian Lipkin

Published in: Virology Journal | Issue 1/2014

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Abstract

Background

Fevers of unknown origin constitute a substantial disease burden in Southeast Asia. In majority of the cases, the cause of acute febrile illness is not identified.

Methods

We used MassTag PCR, a multiplex assay platform, to test for the presence of 15 viral respiratory agents from 85 patients with unexplained respiratory illness representing six disease clusters that occurred in Cambodia between 2009 and 2012.

Results

We detected a virus in 37 (44%) of the cases. Human rhinovirus, the virus detected most frequently, was found in both children and adults. The viruses most frequently detected in children and adults, respectively, were respiratory syncytial virus and enterovirus 68. Sequence analysis indicated that two distinct clades of enterovirus 68 were circulating during this time period.

Conclusions

This is the first report of enterovirus 68 in Cambodia and contributes to the appreciation of this virus as an important respiratory pathogen.
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Metadata
Title
Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia
Authors
Nary Ly
Rafal Tokarz
Nischay Mishra
Stephen Sameroff
Komal Jain
Agus Rachmat
Ung Sam An
Steven Newell
Dustin J Harrison
W Ian Lipkin
Publication date
01-12-2014
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2014
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-014-0224-x

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