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Open Access 01-12-2024 | Multiple Sclerosis | Research

Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity

Authors: Milica Lazarević, Goran Stegnjaić, Bojan Jevtić, Sanja Despotović, Đurđica Ignjatović, Suzana Stanisavljević, Neda Nikolovski, Miljana Momčilović, Graeme L. Fraser, Mirjana Dimitrijević, Đorđe Miljković

Published in: Journal of Neuroinflammation | Issue 1/2024

Abstract

Experimental autoimmune encephalomyelitis (EAE) induced in inbred rodents, i.e., genetically identical animals kept under identical environmental conditions, shows variable clinical outcomes. We investigated such variations of EAE in Dark Agouti rats immunized with spinal cord homogenate and identified four groups: lethal, severe, moderate, and mild, at day 28 post immunization. Higher numbers of CD4⁺ T cells, helper T cells type 1 (Th1) and 17 (Th17) in particular, were detected in the spinal cord of the severe group in comparison with the moderate group. In addition, increased proportion of Th1 and Th17 cells, and heightened levels of interferon (IFN)-γ and interleukin (IL)-6 were detected in the small intestine lamina propria of the severe group. A selective agonist of free fatty acid receptor type 2 (Ffar2) applied orally in the inductive phase of EAE shifted the distribution of the disease outcomes towards milder forms. This effect was paralleled with potentiation of intestinal innate lymphoid cells type 3 (ILC3) regulatory properties, and diminished Th1 and Th17 cell response in the lymph nodes draining the site of immunization. Our results suggest that different clinical outcomes in DA rats are under determinative influence of intestinal ILC3 activity during the inductive phase of EAE.

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Title: Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity
Authors: Milica Lazarević
Goran Stegnjaić
Bojan Jevtić
Sanja Despotović
Đurđica Ignjatović
Suzana Stanisavljević
Neda Nikolovski
Miljana Momčilović
Graeme L. Fraser
Mirjana Dimitrijević
Đorđe Miljković
Publication date: 01-12-2024
Publisher: BioMed Central
Keyword: Multiple Sclerosis
Published in: Journal of Neuroinflammation / Issue 1/2024
Electronic ISSN: 1742-2094
DOI: https://doi.org/10.1186/s12974-024-03017-7

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Increased regulatory activity of intestinal innate lymphoid cells type 3 (ILC3) prevents experimental autoimmune encephalomyelitis severity

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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