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Published in: Virology Journal 1/2008

Open Access 01-12-2008 | Short report

Morphological evidence for phages in Xylella fastidiosa

Authors: Jianchi Chen, Edwin L Civerolo

Published in: Virology Journal | Issue 1/2008

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Abstract

Presumptive phage particles associated with Xylella fastidiosa strain Temecula-1 grown in PW broth were observed by transmission electron microscopy (TEM) in ultrathin sections of bacterial cell-containing low speed centrifugation pellets and in partially purified preparations from CsCl equilibrium centrifugation density gradients. Ultrathin-sectioned cell pellets contained icosahedral particles of about 45 nm in diameter. Samples collected from CsCl density gradients revealed mostly non-tailed icosahedral but also tailed particles. The icosahedral particles could be divided into two types: a large type (about 45 nm) and a small type (about 30 nm). Filamentous phage-like particles (17 × 120 to 6,300 nm) were also observed. The presence of different types of phage-like particles resembling to those in several bacteriophage families provides new physical evidence, in addition to X. fastidiosa genomic information, that X. fastidiosa possesses active phages. This is the first report of phage particles released in X. fastidiosa cultures.
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Literature
1.
go back to reference Wells JM, Raju BC, Hung H-Y, Weisburg WG, Mandelco-Paul L, Brenner BJ: Xylella fastidiosa new-genus new-species gram-negative xylem-limited fastidious plant bacteria related to Xanthomonas-spp. Int J Syst Bacteriol. 1987, 37: 136-143.CrossRef Wells JM, Raju BC, Hung H-Y, Weisburg WG, Mandelco-Paul L, Brenner BJ: Xylella fastidiosa new-genus new-species gram-negative xylem-limited fastidious plant bacteria related to Xanthomonas-spp. Int J Syst Bacteriol. 1987, 37: 136-143.CrossRef
2.
go back to reference Bhattacharyya A, Stilwagen S, Reznik G, et al: Draft sequencing and comparative genomics of Xylella fastidiosa strains reveal novel biological insights. Genome Res. 2002, 12: 1556-1563. 10.1101/gr.370702.PubMedCentralCrossRefPubMed Bhattacharyya A, Stilwagen S, Reznik G, et al: Draft sequencing and comparative genomics of Xylella fastidiosa strains reveal novel biological insights. Genome Res. 2002, 12: 1556-1563. 10.1101/gr.370702.PubMedCentralCrossRefPubMed
3.
go back to reference Harakava R, Gabriel DW: Genetic differences between two strains of Xylella fastidiosa revealed by suppression subtractive hybridization. Appl Environ Microbiol. 2003, 69: 1315-1318. 10.1128/AEM.69.2.1315-1319.2003.PubMedCentralCrossRefPubMed Harakava R, Gabriel DW: Genetic differences between two strains of Xylella fastidiosa revealed by suppression subtractive hybridization. Appl Environ Microbiol. 2003, 69: 1315-1318. 10.1128/AEM.69.2.1315-1319.2003.PubMedCentralCrossRefPubMed
4.
go back to reference Simpson AJ, Reinach FC, Arruda P, Abreu FA, Acencio M, Alvarenga R, Alves LM, Araya JE, Baia GS, Baptista CS, Barros MH, Bonaccorsi ED, Bordin S, Bové JM, Briones MR, Bueno MR, Camargo AA, Camargo LE, Carraro DM, Carrer H, Colauto NB, Colombo C, Costa FF, Costa MC, Costa-Neto CM, Coutinho LL, Cristofani M, Dias-Neto E, Docena C, El-Dorry H, Facincani AP, Ferreira AJ, Ferreira VC, Ferro JA, Fraga JS, França SC, Franco MC, Frohme M, Furlan LR, Garnier M, Goldman GH, Goldman MH, Gomes SL, Gruber A, Ho PL, Hoheisel JD, Junqueira ML, Kemper EL, Kitajima JP, Krieger JE, Kuramae EE, Laigret F, Lambais MR, Leite LC, Lemos EG, Lemos MV, Lopes SA, Lopes CR, Machado JA, Machado MA, Madeira AM, Madeira HM, Marino CL, Marques MV, Martins EA, Martins EM, Matsukuma AY, Menck CF, Miracca EC, Miyaki CY, Monteriro-Vitorello CB, Moon DH, Nagai MA, Nascimento AL, Netto LE, Nhani A, Nobrega FG, Nunes LR, Oliveira MA, de Oliveira MC, de Oliveira RC, Palmieri DA, Paris A, Peixoto BR, Pereira GA, Pereira HA, Pesquero JB, Quaggio RB, Roberto PG, Rodrigues V, de M, Rosa AJ, de Rosa VE, de Sá RG, Santelli RV, Sawasaki HE, da Silva AC, da Silva AM, da Silva FR, da Silva WA, da Silveira JF, Silvestri ML, Siqueira WJ, de Souza AA, de Souza AP, Terenzi MF, Truffi D, Tsai SM, Tsuhako MH, Vallada H, Van Sluys MA, Verjovski-Almeida S, Vettore AL, Zago MA, Zatz M, Meidanis J, Setubal JC: The genome sequence of the plant pathogen Xylella fastidiosa. Nature. 2000, 406: 151-157. 10.1038/35018003.CrossRefPubMed Simpson AJ, Reinach FC, Arruda P, Abreu FA, Acencio M, Alvarenga R, Alves LM, Araya JE, Baia GS, Baptista CS, Barros MH, Bonaccorsi ED, Bordin S, Bové JM, Briones MR, Bueno MR, Camargo AA, Camargo LE, Carraro DM, Carrer H, Colauto NB, Colombo C, Costa FF, Costa MC, Costa-Neto CM, Coutinho LL, Cristofani M, Dias-Neto E, Docena C, El-Dorry H, Facincani AP, Ferreira AJ, Ferreira VC, Ferro JA, Fraga JS, França SC, Franco MC, Frohme M, Furlan LR, Garnier M, Goldman GH, Goldman MH, Gomes SL, Gruber A, Ho PL, Hoheisel JD, Junqueira ML, Kemper EL, Kitajima JP, Krieger JE, Kuramae EE, Laigret F, Lambais MR, Leite LC, Lemos EG, Lemos MV, Lopes SA, Lopes CR, Machado JA, Machado MA, Madeira AM, Madeira HM, Marino CL, Marques MV, Martins EA, Martins EM, Matsukuma AY, Menck CF, Miracca EC, Miyaki CY, Monteriro-Vitorello CB, Moon DH, Nagai MA, Nascimento AL, Netto LE, Nhani A, Nobrega FG, Nunes LR, Oliveira MA, de Oliveira MC, de Oliveira RC, Palmieri DA, Paris A, Peixoto BR, Pereira GA, Pereira HA, Pesquero JB, Quaggio RB, Roberto PG, Rodrigues V, de M, Rosa AJ, de Rosa VE, de Sá RG, Santelli RV, Sawasaki HE, da Silva AC, da Silva AM, da Silva FR, da Silva WA, da Silveira JF, Silvestri ML, Siqueira WJ, de Souza AA, de Souza AP, Terenzi MF, Truffi D, Tsai SM, Tsuhako MH, Vallada H, Van Sluys MA, Verjovski-Almeida S, Vettore AL, Zago MA, Zatz M, Meidanis J, Setubal JC: The genome sequence of the plant pathogen Xylella fastidiosa. Nature. 2000, 406: 151-157. 10.1038/35018003.CrossRefPubMed
5.
go back to reference Van Sluys MA, de Oliveira MC, Monteiro-Vitorello CB, Miyaki CY, Furlan LR, Camargo LE, da Silva AC, Moon DH, Takita MA, Lemos EG, Machado MA, Ferro MI, da Silva FR, Goldman MH, Goldman GH, Lemos MV, El-Dorry H, Tsai SM, Carrer H, Carraro DM, de Oliveira RC, Nunes LR, Siqueira WJ, Coutinho LL, Kimura ET, Ferro ES, Harakava R, Kuramae EE, Marino CL, Giglioti E, Abreu IL, Alves LM, do Amaral AM, Baia GS, Blanco SR, Brito MS, Cannavan FS, Celestino AV, da Cunha AF, Fenille RC, Ferro JA, Formighieri EF, Kishi LT, Leoni SG, Oliveira AR, Rosa VE, Sassaki FT, Sena JA, de Souza AA, Truffi D, Tsukumo F, Yanai GM, Zaros LG, Civerolo EL, Simpson AJ, Almeida NF, Setubal JC, Kitajima JP: Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa. J Bacteriol. 2003, 185: 1018-1026. 10.1128/JB.185.3.1018-1026.2003.PubMedCentralCrossRefPubMed Van Sluys MA, de Oliveira MC, Monteiro-Vitorello CB, Miyaki CY, Furlan LR, Camargo LE, da Silva AC, Moon DH, Takita MA, Lemos EG, Machado MA, Ferro MI, da Silva FR, Goldman MH, Goldman GH, Lemos MV, El-Dorry H, Tsai SM, Carrer H, Carraro DM, de Oliveira RC, Nunes LR, Siqueira WJ, Coutinho LL, Kimura ET, Ferro ES, Harakava R, Kuramae EE, Marino CL, Giglioti E, Abreu IL, Alves LM, do Amaral AM, Baia GS, Blanco SR, Brito MS, Cannavan FS, Celestino AV, da Cunha AF, Fenille RC, Ferro JA, Formighieri EF, Kishi LT, Leoni SG, Oliveira AR, Rosa VE, Sassaki FT, Sena JA, de Souza AA, Truffi D, Tsukumo F, Yanai GM, Zaros LG, Civerolo EL, Simpson AJ, Almeida NF, Setubal JC, Kitajima JP: Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa. J Bacteriol. 2003, 185: 1018-1026. 10.1128/JB.185.3.1018-1026.2003.PubMedCentralCrossRefPubMed
6.
go back to reference Chen J, Civerolo EL, Jarret RL, Van Sluys MA, de Oliveira MC: Genetic discovery in Xylella fastidiosa through sequence analysis of selected randomly amplified polymorphic DNAs. Curr Microbiol. 2005, 50: 78-83. 10.1007/s00284-004-4412-6.CrossRefPubMed Chen J, Civerolo EL, Jarret RL, Van Sluys MA, de Oliveira MC: Genetic discovery in Xylella fastidiosa through sequence analysis of selected randomly amplified polymorphic DNAs. Curr Microbiol. 2005, 50: 78-83. 10.1007/s00284-004-4412-6.CrossRefPubMed
7.
go back to reference Lauzon CR, Miller TA: A survey of insect vectors of Pierce's disease (PD) and PD-infected plants for the presence of bacteriophage that infect Xylella fastidiosa. Proceedings of the Pierces Disease Research Symposium. 2001, San Diego, California, 62-5–7 December 2001 Lauzon CR, Miller TA: A survey of insect vectors of Pierce's disease (PD) and PD-infected plants for the presence of bacteriophage that infect Xylella fastidiosa. Proceedings of the Pierces Disease Research Symposium. 2001, San Diego, California, 62-5–7 December 2001
8.
go back to reference Davis MJ, French WJ, Schaad NW: Axenic culture of the bacteria associated with phony disease of peach and plum leaf scald. Curr Microbiol. 1981, 6: 309-314. 10.1007/BF01566883.CrossRef Davis MJ, French WJ, Schaad NW: Axenic culture of the bacteria associated with phony disease of peach and plum leaf scald. Curr Microbiol. 1981, 6: 309-314. 10.1007/BF01566883.CrossRef
9.
go back to reference Minsavage GV, Thompson CM, Hopkins DL, Leite RMVBC, Stall RE: Development of a polymerase chain reaction protocol for detection of Xylella fastidiosa in plant tissue. Phytopathology. 1994, 84: 456-461. 10.1094/Phyto-84-456.CrossRef Minsavage GV, Thompson CM, Hopkins DL, Leite RMVBC, Stall RE: Development of a polymerase chain reaction protocol for detection of Xylella fastidiosa in plant tissue. Phytopathology. 1994, 84: 456-461. 10.1094/Phyto-84-456.CrossRef
10.
go back to reference Spurr AR: A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969, 26: 31-43. 10.1016/S0022-5320(69)90033-1.CrossRefPubMed Spurr AR: A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969, 26: 31-43. 10.1016/S0022-5320(69)90033-1.CrossRefPubMed
11.
12.
go back to reference Ackermann HW: 5500 Phages examined in the electron microscope. Arch Virol. 2007, 152: 227-243. 10.1007/s00705-006-0849-1.CrossRefPubMed Ackermann HW: 5500 Phages examined in the electron microscope. Arch Virol. 2007, 152: 227-243. 10.1007/s00705-006-0849-1.CrossRefPubMed
13.
go back to reference Kitajima EW, Bakarcic M, Fernandez-Valiela MV: Association of Rickettisalike Bacteria with plum leaf scald disease. Phytopathology. 1975, 65: 476-479.CrossRef Kitajima EW, Bakarcic M, Fernandez-Valiela MV: Association of Rickettisalike Bacteria with plum leaf scald disease. Phytopathology. 1975, 65: 476-479.CrossRef
14.
go back to reference Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. 2001, Cold Spring Harbor, NY, 3 Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. 2001, Cold Spring Harbor, NY, 3
15.
go back to reference Fan FF, Corbett K: Some properties of two bacteriophages of Xanthomonas citri. Phytopathology. 1981, 71: 873- Fan FF, Corbett K: Some properties of two bacteriophages of Xanthomonas citri. Phytopathology. 1981, 71: 873-
16.
go back to reference Klieve AV, Bauchop T: Morphological diversity of ruminal bacteriophages from sheep and cattle. Appl Environ Microbiol. 1988, 54: 1637-1641.PubMedCentralPubMed Klieve AV, Bauchop T: Morphological diversity of ruminal bacteriophages from sheep and cattle. Appl Environ Microbiol. 1988, 54: 1637-1641.PubMedCentralPubMed
17.
go back to reference Meng Y, Li Y, Galvani CD, Hao G, Turner JN, Burr TJ, Hoch HC: Upstream migration of Xylella fastidiosa via pilus-driven twitching motility. J Bacteriol. 2005, 187: 5560-7. 10.1128/JB.187.16.5560-5567.2005.PubMedCentralCrossRefPubMed Meng Y, Li Y, Galvani CD, Hao G, Turner JN, Burr TJ, Hoch HC: Upstream migration of Xylella fastidiosa via pilus-driven twitching motility. J Bacteriol. 2005, 187: 5560-7. 10.1128/JB.187.16.5560-5567.2005.PubMedCentralCrossRefPubMed
18.
go back to reference Mattick JS: Type IV pili and twitching motility. Annu Rev Microbiol. 2002, 56: 289-314. 10.1146/annurev.micro.56.012302.160938.CrossRefPubMed Mattick JS: Type IV pili and twitching motility. Annu Rev Microbiol. 2002, 56: 289-314. 10.1146/annurev.micro.56.012302.160938.CrossRefPubMed
Metadata
Title
Morphological evidence for phages in Xylella fastidiosa
Authors
Jianchi Chen
Edwin L Civerolo
Publication date
01-12-2008
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2008
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-5-75

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