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Virology Journal
Published in: Virology Journal 1/2023

Open Access 01-12-2023 | Research

Morphological, biological, and genomic characterization of Klebsiella pneumoniae phage vB_Kpn_ZC2

Authors: Mohamed S. Fayez, Toka A. Hakim, Bishoy Maher Zaki, Salsabil Makky, Mohamed Abdelmoteleb, Kareem Essam, Anan Safwat, Abdallah S. Abdelsattar, Ayman El-Shibiny

Published in: Virology Journal | Issue 1/2023

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Abstract

Background

Bacteriophages (phages) are one of the most promising alternatives to traditional antibiotic therapies, especially against multidrug-resistant bacteria. Klebsiella pneumoniae is considered to be an opportunistic pathogen that can cause life-threatening infections. Thus, this study aims at the characterization of a novel isolated phage vB_Kpn_ZC2 (ZCKP2, for short).

Methods

The phage ZCKP2 was isolated from sewage water by using the clinical isolate KP/08 as a host strain. The isolated bacteriophage was purified and amplified, followed by testing of its molecular weight using Pulse-Field Gel Electrophoresis (PFGE), transmission electron microscopy, antibacterial activity against a panel of other Klebsiella pneumoniae hosts, stability studies, and whole genome sequencing.

Results

Phage ZCKP2 belongs morphologically to siphoviruses as indicated from the Transmission Electron Microscopy microgram. The Pulsed Field Gel Electrophoresis and the phage sequencing estimated the phage genome size of 48.2 kbp. Moreover, the absence of lysogeny-related genes, antibiotic resistance genes, and virulence genes in the annotated genome suggests that phage ZCKP2 is safe for therapeutic use. Genome-based taxonomic analysis indicates that phage ZCKP2 represents a new family that has not been formally rated yet. In addition, phage ZCKP2 preserved high stability at different temperatures and pH values (-20 − 70 °C and pH 4 – 9). For the antibacterial activity, phage ZCKP2 maintained consistent clear zones on KP/08 bacteria along with other hosts, in addition to effective bacterial killing over time at different MOIs (0.1, 1, and 10). Also, the genome annotation predicted antibacterial lytic enzymes. Furthermore, the topology of class II holins was predicted in some putative proteins with dual transmembrane domains that contribute significantly to antibacterial activity. Phage ZCKP2 characterization demonstrates safety and efficiency against multidrug-resistant K. pneumoniae, hence ZCKP2 is a good candidate for further in vivo and phage therapy clinical applications.
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Literature
1.
Collignon P. Antibiotic resistance: are we all doomed?: antibiotic resistance: are we all doomed? Intern Med J. 2015;45:1109–15.PubMedCrossRef
2.
Ventola CL. The Antibiotic Resistance Crisis. Pharm Ther. 2015;40:277–83.
3.
Vivas R, Barbosa AAT, Dolabela SS, Jain S. Multidrug-Resistant Bacteria and Alternative Methods to Control Them: An Overview. Microb Drug Resist. Mary Ann Liebert, Inc., publishers; 2019;25:890–908.
4.
5.
Dahal RH, Chaudhary DK. Microbial Infections and Antimicrobial Resistance in Nepal: current Trends and Recommendations. Open Microbiol J. 2018;12:230–42.PubMedPubMedCentralCrossRef
6.
Abdelsattar AS, Makky S, Nofal R, Hebishy M, Agwa MM, Aly RG, et al. Enhancement of wound healing via topical application of natural products: in vitro and in vivo evaluations. Arab J Chem. 2022;15:103869.CrossRef
7.
8.
Rossolini GM, Arena F, Pecile P, Pollini S. Update on the antibiotic resistance crisis. Curr Opin Pharmacol. 2014;18:56–60.PubMedCrossRef
9.
10.
Vading M, Nauclér P, Kalin M, Giske CG. Invasive infection caused by Klebsiella pneumoniae is a disease affecting patients with high comorbidity and associated with high long-term mortality. PLoS ONE. 2018;13:e0195258.PubMedPubMedCentralCrossRef
11.
Iredell J, Brown J, Tagg K. Antibiotic resistance in Enterobacteriaceae: mechanisms and clinical implications. BMJ. 2016;352:h6420.PubMedCrossRef
12.
13.
Solomon SL, Oliver KB. Antibiotic resistance threats in the United States: stepping back from the Brink. Am Fam Physician. 2014;89:938–41.PubMed
14.
Willyard C. The drug-resistant bacteria that pose the greatest health threats. Nature. 2017;543:15–5.PubMedCrossRef
15.
Górski A, Międzybrodzki R, Jończyk-Matysiak E, Żaczek M, Borysowski J. Phage-specific diverse effects of bacterial viruses on the immune system. Future Microbiol Future Medicine. 2019;14:1171–4.CrossRef
16.
Dufour N, Debarbieux L. [Phage therapy: a realistic weapon against multidrug resistant bacteria]. Med Sci. 2017;33:410–6.
17.
Kutter E, De Vos D, Gvasalia G, Alavidze Z, Gogokhia L, Kuhl S, et al. Phage therapy in clinical practice: treatment of human infections. Curr Pharm Biotechnol. 2009;11:69–86.CrossRef
18.
Sofy AR, El-Dougdoug NK, Refaey EE, Dawoud RA, Hmed AA. Characterization and full genome sequence of Novel KPP-5 lytic phage against Klebsiella pneumoniae responsible for recalcitrant infection. Biomedicines. 2021;9:342.PubMedPubMedCentralCrossRef
19.
Singh A, Singh AN, Rathor N, Chaudhry R, Singh SK, Nath G. Evaluation of bacteriophage cocktail on Septicemia caused by colistin-resistant Klebsiella pneumoniae in mice Model. Front Pharmacol. 2022;13:778676.PubMedPubMedCentralCrossRef
20.
Anand T, Virmani N, Kumar S, Mohanty AK, Pavulraj S, Bera BC, et al. Phage therapy for treatment of virulent Klebsiella pneumoniae infection in a mouse model. J Glob Antimicrob Resist. 2020;21:34–41.PubMedCrossRef
21.
22.
Pertics BZ, Cox A, Nyúl A, Szamek N, Kovács T, Schneider G. Isolation and characterization of a Novel Lytic bacteriophage against the K2 Capsule-Expressing Hypervirulent Klebsiella pneumoniae strain 52145, and identification of its functional depolymerase. Microorganisms. 2021;9:650.PubMedPubMedCentralCrossRef
23.
Luo Z, Geng S, Lu B, Han G, Wang Y, Luo Y, et al. Isolation, genomic analysis, and preliminary application of a bovine Klebsiella pneumoniae bacteriophage vB_Kpn_B01. Front Vet Sci. 2021;8:622049.PubMedPubMedCentralCrossRef
24.
Harb L, Boeckman J, Newkirk H, Liu M, Gill JJ, Ramsey J. Complete genome sequence of the Novel Klebsiella pneumoniae phage Marfa. Microbiol Resour Announc. 2019;8:e00748–19.PubMedPubMedCentralCrossRef
25.
Komisarova EV, Kislichkina AA, Krasilnikova VM, Bogun AG, Fursova NK, Volozhantsev NV. Complete nucleotide sequence of Klebsiella pneumoniae bacteriophage vB_KpnM_KpV477. Genome Announc. 2017;5:e00694–17.PubMedPubMedCentralCrossRef
26.
Solovieva EV, Myakinina VP, Kislichkina AA, Krasilnikova VM, Verevkin VV, Mochalov VV, et al. Comparative genome analysis of novel podoviruses lytic for hypermucoviscous Klebsiella pneumoniae of K1, K2, and K57 capsular types. Virus Res. 2018;243:10–8.PubMedCrossRef
27.
Li F, Tian F, Nazir A, Sui S, Li M, Cheng D, et al. Isolation and genomic characterization of a novel Autographiviridae bacteriophage IME184 with lytic activity against Klebsiella pneumoniae. Virus Res. 2022;319:198873.PubMedCrossRef
28.
Pu M, Han P, Zhang G, Liu Y, Li Y, Li F, et al. Characterization and comparative Genomics analysis of a New Bacteriophage BUCT610 against Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae. Int J Mol Sci Multidisciplinary Digital Publishing Institute. 2022;23:8040.
29.
Bai J, Zhang F, Liang S, Chen Q, Wang W, Wang Y, et al. Isolation and characterization of vB_kpnM_17 – 11, a novel phage efficient against Carbapenem-Resistant Klebsiella pneumoniae. Front Cell Infect Microbiol. 2022;12:897531.PubMedPubMedCentralCrossRef
30.
Chen X, Tang Q, Li X, Zheng X, Li P, Li M, et al. Isolation, characterization, and genome analysis of bacteriophage P929 that could specifically lyase the KL19 capsular type of Klebsiella pneumoniae. Virus Res. 2022;314:198750.PubMedCrossRef
31.
Han K, Zhu Y, Li F, Li M, An X, Song L et al. Genomic Analysis of Bacteriophage BUCT86 Infecting Klebsiella Pneumoniae.Microbiol Resour Announc. 11:e01238-21.
32.
Habibinava F, Soleimani M, Sabouri S, Zargar M, Zolfaghari MR. Isolating and sequencing vB_Kpn_3, a lytic bacteriophage against multidrug-resistant Klebsiella pneumoniae. Future Microbiol. 2022;17:235–49.PubMedCrossRef
33.
Fang Q, Feng Y, McNally A, Zong Z. Characterization of phage resistance and phages capable of intestinal decolonization of carbapenem-resistant Klebsiella pneumoniae in mice. Commun Biol. 2022;5:48.PubMedPubMedCentralCrossRef
34.
Herridge WP, Shibu P, O’Shea J, Brook TC, Hoyles L. Bacteriophages of Klebsiella spp., their diversity and potential therapeutic uses. J Med Microbiol. 2020;69:176–94.PubMedPubMedCentral
35.
Chhibber S, Nag D, Bansal S. Inhibiting biofilm formation by Klebsiella pneumoniae B5055 using an iron antagonizing molecule and a bacteriophage. BMC Microbiol. 2013;13:174.PubMedPubMedCentralCrossRef
36.
Jamal M, Hussain T, Das CR, Andleeb S. Characterization of Siphoviridae phage Z and studying its efficacy against multidrug-resistant Klebsiella pneumoniae planktonic cells and biofilm. J Med Microbiol Microbiology Society. 2015;64:454–62.CrossRef
37.
Philipson CW, Voegtly LJ, Lueder MR, Long KA, Rice GK, Frey KG, et al. Characterizing phage genomes for therapeutic applications. Viruses. 2018;10:188.PubMedPubMedCentralCrossRef
38.
Klumpp J, Fouts DE, Sozhamannan S. Next generation sequencing technologies and the changing landscape of phage genomics. Bacteriophage. 2012;2:190–9.PubMedPubMedCentralCrossRef
39.
40.
Hesse S, Rajaure M, Wall E, Johnson J, Bliskovsky V, Gottesman S, et al. Phage resistance in Multidrug-Resistant Klebsiella pneumoniae ST258 evolves via diverse mutations that culminate in impaired adsorption. Volume 11. mBio. American Society for Microbiology; 2020. pp. e02530–19.
41.
Fayez MS, Hakim TA, Agwa MM, Abdelmoteleb M, Aly RG, Montaser NN, et al. Topically Applied Bacteriophage to control Multi-Drug resistant Klebsiella pneumoniae infected Wound in a rat model. Volume 10. Antibiotics. Multidisciplinary Digital Publishing Institute; 2021. p. 1048.
42.
Srivastava S, Singh V, Kumar V, Verma PC, Srivastava R, Basu V, et al. Identification of regulatory elements in 16S rRNA gene of Acinetobacter species isolated from water sample. Bioinformation. 2008;3:173–6.PubMedPubMedCentralCrossRef
43.
Abraham O-SJ, Miguel T-S, Inocencio H-C, Blondy C-C. A quick and effective in-house method of DNA purification from agarose gel, suitable for sequencing. 3 Biotech. 2017;7:180.PubMedPubMedCentralCrossRef
44.
Treves DS. Review of three DNA analysis applications for Use in the Microbiology or Genetics Classroom. J Microbiol Biol Educ American Society for Microbiology. 2010;11:186–7.CrossRef
45.
Townsend EM, Kelly L, Gannon L, Muscatt G, Dunstan R, Michniewski S, et al. Isolation and characterization of Klebsiella Phages for Phage Therapy. PHAGE Ther Appl Res. 2021;2:26–42.
46.
Islam MdS, Zhou Y, Liang L, Nime I, Liu K, Yan T, et al. Application of a phage cocktail for control of Salmonella in Foods and reducing Biofilms. Viruses. 2019;11:841.PubMedPubMedCentralCrossRef
47.
Abdelsattar AS, Abdelrahman F, Dawoud A, Connerton IF, El-Shibiny A. Encapsulation of E. coli phage ZCEC5 in chitosan–alginate beads as a delivery system in phage therapy. AMB Express. 2019;9:87.PubMedPubMedCentralCrossRef
48.
Mazzocco A, Waddell TE, Lingohr E, Johnson RP. Enumeration of bacteriophages by the direct plating plaque assay. Methods Mol Biol Clifton NJ. 2009;501:77–80.CrossRef
49.
Abdelrahman F, Rezk N, Fayez MS, Abdelmoteleb M, Atteya R, Elhadidy M, et al. Isolation, characterization, and genomic analysis of three Novel E. coli Bacteriophages that effectively infect E. coli O18. Microorganisms. Volume 10. Multidisciplinary Digital Publishing Institute; 2022. p. 589.
50.
Senczek D, Stephan R, Untermann F. Pulsed-field gel electrophoresis (PFGE) typing of Listeria strains isolated from a meat processing plant over a 2-year period. Int J Food Microbiol. 2000;62:155–9.PubMedCrossRef
51.
Makky S, Rezk N, Abdelsattar AS, Hussein AH, Eid A, Essam K, et al. Characterization of the biosynthesized Syzygium aromaticum-mediated silver nanoparticles and its antibacterial and antibiofilm activity in combination with bacteriophage. Results Chem. 2023;5:100686.CrossRef
52.
53.
Rezk N, Abdelsattar AS, Elzoghby D, Agwa MM, Abdelmoteleb M, Aly RG, et al. Bacteriophage as a potential therapy to control antibiotic-resistant Pseudomonas aeruginosa infection through topical application onto a full-thickness wound in a rat model. J Genet Eng Biotechnol. 2022;20:133.PubMedPubMedCentralCrossRef
54.
Mirzaei MK, Nilsson AS. Isolation of phages for phage therapy: a comparison of Spot tests and efficiency of plating analyses for determination of host range and efficacy. Volume 10. PLOS ONE. Public Library of Science; 2015. p. e0118557.
55.
Kutter E, Sulakvelidze A. BACTERIOPHAGES: BIOLOGY AND APPLICATIONS.:5.
56.
57.
58.
59.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018;35:1547–9.PubMedPubMedCentralCrossRef
60.
Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:4673–80.PubMedPubMedCentralCrossRef
61.
Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y, et al. PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res. 2016;44:W16–21.PubMedPubMedCentralCrossRef
62.
Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, et al. The RAST server: Rapid annotations using Subsystems Technology. BMC Genomics. 2008;9:75.PubMedPubMedCentralCrossRef
63.
Brettin T, Davis JJ, Disz T, Edwards RA, Gerdes S, Olsen GJ, et al. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci Rep Nature Publishing Group. 2015;5:8365.
64.
Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ, Disz T, et al. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res. 2014;42:D206–214.PubMedCrossRef
65.
Olson RD, Assaf R, Brettin T, Conrad N, Cucinell C, Davis JJ, et al. Introducing the bacterial and viral Bioinformatics Resource Center (BV-BRC): a resource combining PATRIC, IRD and ViPR. Nucleic Acids Res. 2023;51:D678–89.PubMedCrossRef
66.
Yukgehnaish K, Rajandas H, Parimannan S, Manickam R, Marimuthu K, Petersen B, et al. PhageLeads: Rapid Assessment of Phage therapeutic suitability using an Ensemble Machine Learning Approach. Viruses. Volume 14. Multidisciplinary Digital Publishing Institute; 2022. p. 342.
67.
68.
Moraru C, Varsani A, Kropinski AM. VIRIDIC—A Novel Tool to calculate the intergenomic similarities of Prokaryote-Infecting Viruses. Viruses. Volume 12. Multidisciplinary Digital Publishing Institute; 2020. p. 1268.
69.
Nishimura Y, Yoshida T, Kuronishi M, Uehara H, Ogata H, Goto S. ViPTree: the viral proteomic tree server. Bioinformatics. 2017;33:2379–80.PubMedCrossRef
70.
Davis P, Seto D, Mahadevan P. CoreGenes5.0: an updated user-friendly webserver for the determination of Core genes from sets of viral and bacterial genomes. Viruses. 2022;14:2534.PubMedPubMedCentralCrossRef
71.
72.
73.
Ferreira RL, da Silva BCM, Rezende GS, Nakamura-Silva R, Pitondo-Silva A, Campanini EB, et al. High prevalence of Multidrug-Resistant Klebsiella pneumoniae harboring several virulence and β-Lactamase encoding genes in a brazilian Intensive Care Unit. Front Microbiol. 2018;9:3198.PubMedCrossRef
74.
Derakhshan S, Najar Peerayeh S, Bakhshi B. Association between Presence of virulence genes and Antibiotic Resistance in Clinical Klebsiella Pneumoniae isolates. Lab Med. 2016;47:306–11.PubMedCrossRef
75.
76.
Dufour N, Debarbieux L. [Phage therapy: a realistic weapon against multidrug resistant bacteria]. Med Sci MS. 2017;33:410–6.
77.
Wintachai P, Naknaen A, Thammaphet J, Pomwised R, Phaonakrop N, Roytrakul S, et al. Characterization of extended-spectrum-β-lactamase producing Klebsiella pneumoniae phage KP1801 and evaluation of therapeutic efficacy in vitro and in vivo. Sci Rep. 2020;10:11803.PubMedPubMedCentralCrossRef
78.
Feng J, Gao L, Li L, Zhang Z, Wu C, Li F, et al. Characterization and genome analysis of novel Klebsiella phage BUCT556A with lytic activity against carbapenemase-producing Klebsiella pneumoniae. Virus Res. 2021;303:198506.PubMedCrossRef
79.
Tabassum R, Shafique M, Khawaja KA, Alvi IA, Rehman Y, Sheik CS, et al. Complete genome analysis of a Siphoviridae phage TSK1 showing biofilm removal potential against Klebsiella pneumoniae. Sci Rep Nature Publishing Group. 2018;8:17904.
80.
Juergensmeyer MA, Nelson ES, Juergensmeyer EA. Shaking alone, without concurrent aeration, affects the growth characteristics of Escherichia coli. Lett Appl Microbiol. 2007;45:179–83.PubMedCrossRef
81.
Horváth M, Kovács T, Koderivalappil S, Ábrahám H, Rákhely G, Schneider G. Identification of a newly isolated lytic bacteriophage against K24 capsular type, carbapenem resistant Klebsiella pneumoniae isolates. Sci Rep Nature Publishing Group. 2020;10:5891.
82.
Li M, Guo M, Chen L, Zhu C, Xiao Y, Li P, et al. Isolation and characterization of Novel Lytic Bacteriophages infecting Epidemic Carbapenem-Resistant Klebsiella pneumoniae strains. Front Microbiol. 2020;11:1554.PubMedPubMedCentralCrossRef
83.
Lu B, Yao X, Han G, Luo Z, Zhang J, Yong K, et al. Isolation of Klebsiella pneumoniae phage vB_KpnS_MK54 and pathological Assessment of Endolysin in the treatment of Pneumonia mice Model. Front Microbiol. 2022;13:854908.PubMedPubMedCentralCrossRef
84.
Krasowska A, Biegalska A, Augustyniak D, Łoś M, Richert M, Łukaszewicz M. Isolation and characterization of phages infecting Bacillus subtilis. BioMed Res Int Hindawi. 2015;2015:e179597.
85.
Anand T, Virmani N, Kumar S, Mohanty AK, Pavulraj S, Bera BCh, et al. Phage therapy for treatment of virulent Klebsiella pneumoniae infection in a mouse model. J Glob Antimicrob Resist. 2020;21:34–41.PubMedCrossRef
86.
Cahill J, Young R. Phage lysis: multiple genes for multiple barriers. Adv Virus Res. 2019;103:33–70.PubMedCrossRef
87.
Vukov N, Scherer S, Hibbert E, Loessner MJ. Functional analysis of heterologous holin proteins in a λ∆S genetic background. FEMS Microbiol Lett. 2000;184:179–86.PubMed
88.
89.
90.
91.
92.
Grose JH, Casjens SR. Understanding the enormous diversity of bacteriophages: the tailed phages that infect the bacterial family Enterobacteriaceae. Virology. 2014;468–470:421–43.PubMedCrossRef
93.
Adriaenssens EM, Sullivan MB, Knezevic P, van Zyl LJ, Sarkar BL, Dutilh BE, et al. Taxonomy of prokaryotic viruses: 2018–2019 update from the ICTV bacterial and archaeal viruses Subcommittee. Arch Virol. 2020;165:1253–60.PubMedCrossRef
94.
Osama DM, Zaki BM, Khalaf WS, Mohamed MY, Tawfick MM, Amin HM. Occurrence and Molecular Study of Hypermucoviscous/Hypervirulence Trait in Gut Commensal K. pneumoniae from Healthy Subjects. Microorganisms. 2023;11(3):704.
95.
Zaki BM, Mohamed AA, Dawoud A, Essam K, Hammouda ZK, Abdelsattar AS, El-Shibiny A. Isolation, screening and characterization of phage. 2023.
Metadata
Title
Morphological, biological, and genomic characterization of Klebsiella pneumoniae phage vB_Kpn_ZC2
Authors
Mohamed S. Fayez
Toka A. Hakim
Bishoy Maher Zaki
Salsabil Makky
Mohamed Abdelmoteleb
Kareem Essam
Anan Safwat
Abdallah S. Abdelsattar
Ayman El-Shibiny
Publication date
01-12-2023
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2023
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/s12985-023-02034-x

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