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European Journal of Clinical Microbiology & Infectious Diseases
Published in: European Journal of Clinical Microbiology & Infectious Diseases 9/2012

01-09-2012 | Article

Molecular characterization of adult-colonizing Streptococcus agalactiae from an area-based surveillance study in Romania

Authors: C.-R. Usein, L. Grigore, R. Georgescu, V. Cristea, M. Bãltoiu, M. Strãuţ

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 9/2012

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Abstract

One hundred and forty-eight colonizing isolates from adult Romanian women were conventionally serotyped and screened for antibiotic resistance. Capsular type assignment by multiplex polymerase chain reaction (PCR) was performed for nonserotypeable isolates. Tetracycline and macrolide resistance genes (tetM, tetO, tetL, ermA, ermB, and mefA) including tetM gene association with conjugative elements of the Tn916 family were searched. Molecular typing included PCR screening for major surface protein antigen genes (bac, bca, alp1, alp2/3, alp4, and rib), mobile genetic elements (GBSi1 and IS1548), and rapid detection of hypervirulent clone ST-17. Genetic diversity was assessed by pulsed field gel electrophoresis (PFGE) analysis of SmaI macrorestriction patterns. Among the colonizing isolates studied, serotypes V and III predominated and high rates of tetracycline and macrolide resistance were observed. The tetM gene occurred in 140 tetracycline-resistant isolates and was associated with the int-Tn916 gene in 94 of them. Most of the isolates displayed a constitutive MLSB phenotype (38/46 isolates) and harbored the ermB gene. rib, alp2/3, and alp1 were the most common surface protein genes detected. Either IS1548 or GBSi1 intron were detected in almost half of the isolates and nine serotype III isolates belonged to clone ST-17. PFGE analysis of SmaI macrorestriction patterns, obtained from 118 isolates, revealed an apparent genetic diversity.
Literature
1.
Shet A, Ferrieri P (2004) Neonatal & maternal group B streptococcal infections: a comprehensive review. Indian J Med Res 120(3):141–150PubMed
2.
Edwards MS, Kasper DL, Jennings HJ, Baker CJ, Nicholson-Weller A (1982) Capsular sialic acid prevents activation of the alternative complement pathway by type III, group B streptococci. J Immunol 128(3):1278–1283PubMed
3.
Lindahl G, Stålhammar-Carlemalm M, Areschoug T (2005) Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens. Clin Microbiol Rev 18:102–127PubMedCrossRef
4.
Doran KS, Nizet V (2004) Molecular pathogenesis of neonatal group B streptococcal infection: no longer in its infancy. Mol Microbiol 54(1):23–31PubMedCrossRef
5.
Granlund M, Oberg L, Sellin M, Norgren M (1998) Identification of a novel insertion element, IS1548, in group B streptococci, predominantly in strains causing endocarditis. J Infect Dis 177:967–976PubMedCrossRef
6.
Bohnsack JF, Whiting AA, Bradford RD, Van Frank BK, Takahashi S, Adderson EE (2002) Long-range mapping of the Streptococcus agalactiae phylogenetic lineage restriction digest pattern type III-3 reveals clustering of virulence genes. Infect Immun 70(1):134–139PubMedCrossRef
7.
Héry-Arnaud G, Bruant G, Lanotte P, Brun S, Rosenau A, van der Mee-Marquet N, Quentin R, Mereghetti L (2005) Acquisition of insertion sequences and the GBSi1 intron by Streptococcus agalactiae isolates correlates with the evolution of the species. J Bacteriol 187(17):6248–6252PubMedCrossRef
8.
Skoff TH, Farley MM, Petit S, Craig AS, Schaffner W, Gershman K, Harrison LH, Lynfield R, Mohle-Boetani J, Zansky S, Albanese BA, Stefonek K, Zell ER, Jackson D, Thompson T, Schrag SJ (2009) Increasing burden of invasive group B streptococcal disease in nonpregnant adults, 1990–2007. Clin Infect Dis 49(1):85–92PubMedCrossRef
9.
Castor ML, Whitney CG, Como-Sabetti K, Facklam RR, Ferrieri P, Bartkus JM, Juni BA, Cieslak PR, Farley MM, Dumas NB, Schrag SJ, Lynfield R (2008) Antibiotic resistance patterns in invasive group B streptococcal isolates. Infect Dis Obstet Gynecol 2008:727505PubMedCrossRef
10.
Paoletti LC, Kasper DL (2003) Glycoconjugate vaccines to prevent group B streptococcal infections. Expert Opin Biol Ther 3(6):975–984PubMedCrossRef
11.
Margarit I, Rinaudo CD, Galeotti CL, Maione D, Ghezzo C, Buttazzoni E, Rosini R, Runci Y, Mora M, Buccato S, Pagani M, Tresoldi E, Berardi A, Creti R, Baker CJ, Telford JL, Grandi G (2009) Preventing bacterial infections with pilus-based vaccines: the group B streptococcus paradigm. J Infect Dis 199(1):108–115PubMedCrossRef
12.
Clinical and Laboratory Standards Institute (CLSI) (2010) Performance standards for antimicrobial susceptibility testing; Twentieth informational supplement. M100–S20. CLSI, Wayne, PA
13.
Poyart C, Tazi A, Réglier-Poupet H, Billoët A, Tavares N, Raymond J, Trieu-Cuot P (2007) Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol 45(6):1985–1988PubMedCrossRef
14.
Dmitriev A, Hu YY, Shen AD, Suvorov A, Yang YH (2002) Chromosomal analysis of group B streptococcal clinical strains; bac gene-positive strains are genetically homogenous. FEMS Microbiol Lett 208:93–98PubMedCrossRef
15.
Creti R, Fabretti F, Orefici G, von Hunolstein C (2004) Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes. J Clin Microbiol 42(3):1326–1329PubMedCrossRef
16.
Olsvik B, Olsen I, Tenover FC (1995) Detection of tet(M) and tet(O) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease. Oral Microbiol Immunol 10(2):87–92PubMedCrossRef
17.
Poyart C, Jardy L, Quesne G, Berche P, Trieu-Cuot P (2003) Genetic basis of antibiotic resistance in Streptococcus agalactiae strains isolated in a French hospital. Antimicrob Agents Chemother 47(2):794–797PubMedCrossRef
18.
Sutcliffe J, Grebe T, Tait-Kamradt A, Wondrack L (1996) Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother 40(11):2562–2566PubMed
19.
Al Safadi R, Amor S, Héry-Arnaud G, Spellerberg B, Lanotte P, Mereghetti L, Gannier F, Quentin R, Rosenau A (2010) Enhanced expression of lmb gene encoding laminin-binding protein in Streptococcus agalactiae strains harboring IS1548 in scpB-lmb intergenic region. PLoS One 5(5):e10794PubMedCrossRef
20.
Lamy MC, Dramsi S, Billoët A, Réglier-Poupet H, Tazi A, Raymond J, Guérin F, Couvé E, Kunst F, Glaser P, Trieu-Cuot P, Poyart C (2006) Rapid detection of the “highly virulent” group B Streptococcus ST-17 clone. Microbes Infect 8(7):1714–1722PubMedCrossRef
21.
Codruţa-Romaniţa U, Mariana-Silvia C, Vasilica U, Maria G, Anca P, Monica S (2009) Genetic heterogeneity of group B streptococci isolated from Romanian pregnant women. Roum Arch Microbiol Immunol 68(2):83–88PubMed
22.
van Belkum A, Tassios PT, Dijkshoorn L, Haeggman S, Cookson B, Fry NK, Fussing V, Green J, Feil E, Gerner-Smidt P, Brisse S, Struelens M; European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group on Epidemiological Markers (ESGEM) (2007) Guidelines for the validation and application of typing methods for use in bacterial epidemiology. Clin Microbiol Infect 13(Suppl 3):1–46PubMedCrossRef
23.
Cristea VC, Duţă M, Neacşu G (2011) Screening for group B streptococcus: a private laboratory experience. Roum Arch Microbiol Immunol 70(2):65–68PubMed
24.
Gulan L, Mihalcu F, Ungureanu V (1992) Group B streptococci implicated in the etiology of acute pneumopathies in neonates. Roum Arch Microbiol Immunol 51(3):165–170PubMed
25.
Usein CR, Petrini A, Georgescu R, Grigore L, Străuţ M, Ungureanu V (2009) Group B streptococcus colonization of Romanian women: phenotypic traits of isolates from vaginal swabs. Roum Arch Microbiol Immunol 68(4):235–239PubMed
26.
Afshar B, Broughton K, Creti R, Decheva A, Hufnagel M, Kriz P, Lambertsen L, Lovgren M, Melin P, Orefici G, Poyart C, Radtke A, Rodriguez-Granger J, Sørensen UB, Telford J, Valinsky L, Zachariadou L; Members of the DEVANI Study Group, Efstratiou A (2011) International external quality assurance for laboratory identification and typing of Streptococcus agalactiae (Group B streptococci). J Clin Microbiol 49(4):1475–1482PubMedCrossRef
27.
Brzychczy-Włoch M, Gosiewski T, Bodaszewska-Lubas M, Adamski P, Heczko PB (2012) Molecular characterization of capsular polysaccharides and surface protein genes in relation to genetic similarity of group B streptococci isolated from Polish pregnant women. Epidemiol Infect 140:329–336PubMedCrossRef
28.
Gherardi G, Imperi M, Baldassarri L, Pataracchia M, Alfarone G, Recchia S, Orefici G, Dicuonzo G, Creti R (2007) Molecular epidemiology and distribution of serotypes, surface proteins, and antibiotic resistance among group B streptococci in Italy. J Clin Microbiol 45(9):2909–2916PubMedCrossRef
29.
Persson E, Berg S, Bevanger L, Bergh K, Valsö-Lyng R, Trollfors B (2008) Characterisation of invasive group B streptococci based on investigation of surface proteins and genes encoding surface proteins. Clin Microbiol Infect 14(1):66–73PubMedCrossRef
30.
Mahillon J, Léonard C, Chandler M (1999) IS elements as constituents of bacterial genomes. Res Microbiol 150(9–10):675–687PubMedCrossRef
31.
Martínez-Abarca F, Toro N (2000) Group II introns in the bacterial world. Mol Microbiol 38(5):917–926PubMedCrossRef
32.
Glaser P, Rusniok C, Buchrieser C, Chevalier F, Frangeul L, Msadek T, Zouine M, Couvé E, Lalioui L, Poyart C, Trieu-Cuot P, Kunst F (2002) Genome sequence of Streptococcus agalactiae, a pathogen causing invasive neonatal disease. Mol Microbiol 45(6):1499–1513PubMedCrossRef
33.
Tettelin H, Masignani V, Cieslewicz MJ, Donati C, Medini D, Ward NL, Angiuoli SV, Crabtree J, Jones AL, Durkin AS, Deboy RT, Davidsen TM, Mora M, Scarselli M, Margarit y Ros I, Peterson JD, Hauser CR, Sundaram JP, Nelson WC, Madupu R, Brinkac LM, Dodson RJ, Rosovitz MJ, Sullivan SA, Daugherty SC, Haft DH, Selengut J, Gwinn ML, Zhou L, Zafar N, Khouri H, Radune D, Dimitrov G, Watkins K, O’Connor KJ, Smith S, Utterback TR, White O, Rubens CE, Grandi G, Madoff LC, Kasper DL, Telford JL, Wessels MR, Rappuoli R, Fraser CM (2005) Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: implications for the microbial “pan-genome”. Proc Natl Acad Sci U S A 102(39):13950–13955PubMedCrossRef
34.
Kong F, Martin D, James G, Gilbert GL (2003) Towards a genotyping system for Streptococcus agalactiae (group B streptococcus): use of mobile genetic elements in Australasian invasive isolates. J Med Microbiol 52(Pt 4):337–344PubMedCrossRef
35.
van der Mee-Marquet N, Jouannet C, Domelier AS, Arnault L, Lartigue MF, Quentin R (2009) Genetic diversity of Streptococcus agalactiae strains and density of vaginal carriage. J Med Microbiol 58(Pt 2):169–173PubMedCrossRef
36.
Granlund M, Michel F, Norgren M (2001) Mutually exclusive distribution of IS1548 and GBSi1, an active group II intron identified in human isolates of group B streptococci. J Bacteriol 183(8):2560–2569PubMedCrossRef
37.
Héry-Arnaud G, Bruant G, Lanotte P, Brun S, Picard B, Rosenau A, van der Mee-Marquet N, Rainard P, Quentin R, Mereghetti L (2007) Mobile genetic elements provide evidence for a bovine origin of clonal complex 17 of Streptococcus agalactiae. Appl Environ Microbiol 73(14):4668–4672PubMedCrossRef
38.
Skov Sørensen UB, Poulsen K, Ghezzo C, Margarit I, Kilian M (2010) Emergence and global dissemination of host-specific Streptococcus agalactiae clones. mBio 1(3):e00178–10CrossRef
39.
Garland SM, Cottrill E, Markowski L, Pearce C, Clifford V, Ndisang D, Kelly N, Daley AJ; Australasian Group for Antimicrobial Resistance-GBS Resistance Study Group (2011) Antimicrobial resistance in group B streptococcus: the Australian experience. J Med Microbiol 60(Pt 2):230–235PubMedCrossRef
40.
Granlund M, Axemo P, Bremme K, Bryngelsson AL, Carlsson Wallin M, Ekström CM, Håkansson S, Jacobsson B, Källén K, Spetz E, Tessin I; Swedish Working Group for the Prevention of Perinatal Group B Streptococcal Infections (2011) Antimicrobial resistance in colonizing group B Streptococci before the implementation of a Swedish intrapartum antibiotic prophylaxis program. Eur J Clin Microbiol Infect Dis 29(10):1195–1201CrossRef
41.
Torres RS, Torres RP, Smeesters PR, Palmeiro JK, de Messias-Reason IJ, Dalla-Costa LM (2011) Group a streptococcus antibiotic resistance in southern Brazil: a 17-year surveillance study. Microb Drug Resist 17(2):313–319PubMedCrossRef
42.
Wehbeh W, Rojas-Diaz R, Li X, Mariano N, Grenner L, Segal-Maurer S, Tommasulo B, Drlica K, Urban C, Rahal JJ (2005) Fluoroquinolone-resistant Streptococcus agalactiae: epidemiology and mechanism of resistance. Antimicrob Agents Chemother 49(6):2495–2497PubMedCrossRef
43.
Domelier AS, van der Mee-Marquet N, Arnault L, Mereghetti L, Lanotte P, Rosenau A, Lartigue MF, Quentin R (2008) Molecular characterization of erythromycin-resistant Streptococcus agalactiae strains. J Antimicrob Chemother 62(6):1227–1233PubMedCrossRef
44.
Florindo C, Viegas S, Paulino A, Rodrigues E, Gomes JP, Borrego MJ (2010) Molecular characterization and antimicrobial susceptibility profiles in Streptococcus agalactiae colonizing strains: association of erythromycin resistance with subtype III-1 genetic clone family. Clin Microbiol Infect 16(9):1458–1463PubMedCrossRef
45.
Sadowy E, Matynia B, Hryniewicz W (2010) Population structure, virulence factors and resistance determinants of invasive, non-invasive and colonizing Streptococcus agalactiae in Poland. J Antimicrob Chemother 65(9):1907–1914PubMedCrossRef
46.
de Azavedo JC, McGavin M, Duncan C, Low DE, McGeer A (2001) Prevalence and mechanisms of macrolide resistance in invasive and noninvasive group B streptococcus isolates from Ontario, Canada. Antimicrob Agents Chemother 45(12):3504–3508PubMedCrossRef
47.
Bozdogan B, Berrezouga L, Kuo MS, Yurek DA, Farley KA, Stockman BJ, Leclercq R (1999) A new resistance gene, linB, conferring resistance to lincosamides by nucleotidylation in Enterococcus faecium HM1025. Antimicrob Agents Chemother 43(4):925–929PubMed
48.
Malbruny B, Werno AM, Anderson TP, Murdoch DR, Leclercq R (2004) A new phenotype of resistance to lincosamide and streptogramin A-type antibiotics in Streptococcus agalactiae in New Zealand. J Antimicrob Chemother 54(6):1040–1044PubMedCrossRef
49.
Pillai P, Srinivasan U, Zhang L, Borchardt SM, Debusscher J, Marrs CF, Foxman B (2009) Streptococcus agalactiae pulsed-field gel electrophoresis patterns cross capsular types. Epidemiol Infect 137(10):1420–1425PubMedCrossRef
50.
Imperi M, Gherardi G, Berardi A, Baldassarri L, Pataracchia M, Dicuonzo G, Orefici G, Creti R (2011) Invasive neonatal GBS infections from an area-based surveillance study in Italy. Clin Microbiol Infect 17(12):1834–1839PubMedCrossRef
Metadata
Title
Molecular characterization of adult-colonizing Streptococcus agalactiae from an area-based surveillance study in Romania
Authors
C.-R. Usein
L. Grigore
R. Georgescu
V. Cristea
M. Bãltoiu
M. Strãuţ
Publication date
01-09-2012
Publisher
Springer-Verlag
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 9/2012
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-012-1569-y

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