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Malaria Journal
Published in: Malaria Journal 1/2018

Open Access 01-12-2018 | Review

Molecular approaches to determine the multiplicity of Plasmodium infections

Authors: Daibin Zhong, Cristian Koepfli, Liwang Cui, Guiyun Yan

Published in: Malaria Journal | Issue 1/2018

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Abstract

Multiplicity of infection (MOI), also termed complexity of infection (COI), is defined as the number of genetically distinct parasite strains co-infecting a single host, which is an important indicator of malaria epidemiology. PCR-based genotyping often underestimates MOI. Next generation sequencing technologies provide much more accurate and genome-wide characterization of polyclonal infections. However, complete haplotype characterization of multiclonal infections remains a challenge due to PCR artifacts and sequencing errors, and requires efficient computational tools. In this review, the advantages and limitations of current molecular approaches to determine multiplicity of malaria parasite infection are discussed.
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Metadata
Title
Molecular approaches to determine the multiplicity of Plasmodium infections
Authors
Daibin Zhong
Cristian Koepfli
Liwang Cui
Guiyun Yan
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2018
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/s12936-018-2322-5

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