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European Journal of Nutrition
Published in: European Journal of Nutrition 4/2016

01-06-2016 | Original Contribution

Moderately increased maternal dietary energy intake delays foetal skeletal muscle differentiation and maturity in pigs

Authors: Tiande Zou, Dongting He, Bing Yu, Jie Yu, Xiangbing Mao, Ping Zheng, Jun He, Zhiqing Huang, Yan Shu, Yue Liu, Daiwen Chen

Published in: European Journal of Nutrition | Issue 4/2016

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Abstract

Objectives

This study aimed to evaluate the effects of moderately increased maternal dietary energy intake during gestation on foetal skeletal muscle development and metabolism with pig as a model.

Methods

Twelve primiparous purebred Large White sows (initial body weight 135.5 ± 1.6 kg) were allocated to one of two energy intake treatments: normal-energy-intake group (Con, 30.96 MJ DE/day) as recommended by the National Research Council (NRC; 2012) and high-energy-intake group (HE, 34.15 MJ DE/day). The nutritional treatments were introduced from mating to day 90 of gestation. On day 90 of gestation, foetuses were examined by morphological, biochemical and molecular analysis of the longissimus muscle. Umbilical vein serum hormones were measured.

Results

Sow body weight was increased in HE group compared with Con group (P < 0.05), whereas foetal myofibre density was decreased (P < 0.05). Meanwhile, protein concentration, creatine kinase and lactate dehydrogenase activities and umbilical vein serum triiodothyronine (T3) concentration were decreased in HE foetuses (P < 0.05). Maternal HE diets decreased the mRNA abundance of muscle growth-related genes, myosin heavy-chain (MYH/MyHC) genes (MYH2 and MYH1) and insulin-like growth factor 1 and insulin growth factor-binding protein 5 (P < 0.05). Furthermore, the protein expressions of myogenic differentiation factor 1, myogenin and fast-MyHC isoforms were reduced in HE foetuses (P < 0.05).

Conclusion

Our results suggest that moderately increased maternal dietary energy intake delays the differentiation and maturation in skeletal muscle of the foetus on day 90 of gestation.
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Metadata
Title
Moderately increased maternal dietary energy intake delays foetal skeletal muscle differentiation and maturity in pigs
Authors
Tiande Zou
Dongting He
Bing Yu
Jie Yu
Xiangbing Mao
Ping Zheng
Jun He
Zhiqing Huang
Yan Shu
Yue Liu
Daiwen Chen
Publication date
01-06-2016
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Nutrition / Issue 4/2016
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-015-0996-9

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