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Intensive Care Medicine

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01-10-2011 | Original

Mitochondrial DNA haplogroup R in the Han population and recovery from septic encephalopathy

Authors: Yi Yang, Ping Zhang, Rong Lv, Qiang He, Yiling Zhu, Xianghong Yang, Jianghua Chen

Published in: Intensive Care Medicine | Issue 10/2011

Abstract

Purpose

To determine whether the main mitochondrial DNA (mtDNA) haplogroups of the Han people are associated with neurological recovery from septic encephalopathy.

Methods

We studied 137 individuals with septic encephalopathy who were sequentially admitted to the intensive care unit or the emergency intensive care unit at the First Affiliated Hospital, College of Medicine, Zhejiang University, and the People’s Hospital of Zhejiang Province. Demographic and clinical data were recorded along with clinical outcome over 28 days. The Glasgow coma scale (GCS) score was calculated daily until it reached 15 or until the patient died during the 28-day period. Follow-up was completed for all study participants. We then determined the mtDNA haplogroups of the patients by analyzing sequences of hypervariable mtDNA segments and testing diagnostic polymorphisms in the mtDNA coding region with DNA probes.

Results

MtDNA haplogroup R, one of the main mtDNA haplogroups of the Han people, was a strong independent predictor of outcome following septic encephalopathy, conferring a 4.053-fold (95% CI 1.803–9.110, p = 0.001) increased chance of neurological recovery within 28 days compared with those with a non-R mtDNA haplogroup.

Conclusion

In the Han population, mtDNA haplogroup R is a strong independent predictor of the outcome of septic encephalopathy, conferring an increased chance of neurological recovery compared with individuals with a non-R haplogroup. Our results provide potential insights into the mechanisms involved in septic encephalopathy, and reveal that the mtDNA haplogroup R is an independent predictor of the outcome of septic encephalopathy.

Szatmári S, Végh T, Csomós A, Hallay J, Takács I, Molnár C, Fülesdi B (2010) Impaired cerebrovascular reactivity in sepsis-associated encephalopathy studied by acetazolamide test. Crit Care 14:R50PubMedCrossRef

Papadopoulos MC, Davies DC, Moss RF, Tighe D, Bennett ED (2000) Pathophysiology of septic encephalopathy: a review. Crit Care Med 28:3019–3024PubMedCrossRef

Otero RM, Nguyen HB, Huang DT, Gaieski DF, Goyal M, Gunnerson KJ, Trzeciak S, Sherwin R, Holthaus CV, Osborn T, Rivers EP (2006) Early goal-directed therapy in severe sepsis and septic shock revisited: concepts, controversies, and contemporary findings. Chest 130:1579–1595PubMedCrossRef

Milbrandt EB, Angus DC (2006) Bench-to-bedside review: critical illness-associated cognitive dysfunction – mechanisms, markers, and emerging therapeutics. Crit Care 10:238PubMedCrossRef

Crouser ED, Julian MW, Huff JE, Mandich DV, Green-Church KB (2006) A proteomic analysis of liver mitochondria during acute endotoxemia. Intensive Care Med 32:1252–1262PubMedCrossRef

Fink MP (2001) Cytopathic hypoxia. Mitochondrial dysfunction as mechanism contributing to organ dysfunction in sepsis. Crit Care Clin 17:219–237PubMedCrossRef

Costa J, Santiago AP, Amâncio RT, Galina A, Oliveira MF, Bozza FA (2008) Sepsis induces brain mitochondrial dysfunction. Crit Care Med 36:1925–1932CrossRef

Spapen H, Nguyen DN, Troubleyn J, Huyghens L, Schiettecatte J (2010) Drotrecogin alfa (activated) may attenuate severe sepsis-associated encephalopathy in clinical septic shock. Crit Care 14:R54PubMedCrossRef

Barichello T, Fortunato JJ, Vitali AM, Feier G, Reinke A, Moreira JC, Quevedo J, Dal-Pizzol F (2006) Oxidative variables in the rat brain after sepsis induced by cecal ligation and perforation. Crit Care Med 34:886–889PubMedCrossRef

10.

Halliwell B (2006) Oxidative stress and neurodegeneration: where are we now? J Neurochem 97:1634–1658PubMedCrossRef

11.

Gellerich FN, Trumbeckaite S, Opalka JR, Gellerich JF, Chen Y, Neuhof C, Redl H, Werdan K, Zierz S (2002) Mitochondrial dysfunction in sepsis: evidence from bacteraemic baboons and endotoxaemic rabbits. Biosci Rep 22:99–113PubMedCrossRef

12.

Muravchick S, Levy RJ (2006) Clinical implications of mitochondrial dysfunction. Anesthesiology 105:819–837PubMedCrossRef

13.

Pyle A, Burn DJ, Gordon C, Swan C, Chinnery PF, Baudouin SV (2010) Fall in circulating mononuclear cell mitochondrial DNA content in human sepsis. Intensive Care Med 36:956–962PubMedCrossRef

14.

Ruiz-Pesini E, Mishmar D, Brandon M, Procaccio V, Wallace DC (2004) Effects of purifying and adaptive selection on regional variation in human mtDNA. Science 303:223–226PubMedCrossRef

15.

Ruiz-Pesini E, Lapeña AC, Díez-Sánchez C, Pérez-Martos A, Montoya J, Alvarez E, Díaz M, Urriés A, Montoro L, López-Pérez MJ, Enríquez JA (2000) Human mtDNA haplogroups associated with a high or reduced spermatozoa motility. Am J Hum Genet 67:682–696PubMedCrossRef

16.

van der Walt JM, Dementieva YA, Martin ER, Scott WK, Nicodemus KK, Kroner CC, Welsh-Bohmer KA, Saunders AM, Roses AD, Small GW, Schmechel DE, Murali Doraiswamy P, Gilbert JR, Haines JL, Vance JM, Pericak-Vance MA (2004) Analysis of European mitochondrial haplogroups with Alzheimer disease risk. Neurosci Lett 365:28–32PubMedCrossRef

17.

Pyle A, Foltynie T, Tiangyou W, Lambert C, Keers SM, Allcock LM, Davison J, Lewis SJ, Perry RH, Barker R, Burn DJ, Chinnery PF (2005) Mitochondrial DNA haplogroup cluster UKJT reduces the risk of PD. Ann Neurol 57:564–567PubMedCrossRef

18.

Ghezzi D, Marelli C, Achilli A, Goldwurm S, Pezzoli G, Barone P, Pellecchia MT, Stanzione P, Brusa L, Bentivoglio AR, Bonuccelli U, Petrozzi L, Abbruzzese G, Marchese R, Cortelli P, Grimaldi D, Martinelli P, Ferrarese C, Garavaglia B, Sangiorgi S, Carelli V, Torroni A, Albanese A, Zeviani M (2005) Mitochondrial DNA haplogroup K is associated with a lower risk of Parkinson’s disease in Italians. Eur J Hum Genet 13:748–752PubMedCrossRef

19.

Baudouin SV, Saunders D, Tiangyou W, Elson JL, Poynter J, Pyle A, Keers S, Turnbull DM, Howell N, Chinnery PF (2005) Mitochondrial DNA and survival after sepsis: a prospective study. Lancet 366:2118–2121PubMedCrossRef

20.

Yang Y, Shou Z, Zhang P, He Q, Xiao H, Xu Y, Li C, Chen J (2008) Mitochondrial DNA haplogroup R predicts survival advantage in severe sepsis in the Han population. Genet Med 10:187–192PubMedCrossRef

21.

Yao YG, Kong QP, Bandelt HJ, Kivisild T, Zhang YP (2002) Phylogeographic differentiation of mitochondrial DNA in Han Chinese. Am J Hum Genet 70:635–651PubMedCrossRef

22.

Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N (1999) Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet 23:147PubMedCrossRef

23.

Kong QP, Bandelt HJ, Sun C, Yao YG, Salas A, Achilli A, Wang CY, Zhong L, Zhu CL, Wu SF, Torroni A, Zhang YP (2006) Updating the East Asian mtDNA phylogeny: a prerequisite for the identification of pathogenic mutations. Hum Mol Genet 15:2076–2086PubMedCrossRef

24.

Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, Cohen J, Opal SM, Vincent JL, Ramsay G; International Sepsis Definitions Conference (2003) 2001 SCCM/ESICM/ACCP/ATS/SIS International Sepsis Definitions Conference. Intensive Care Med 29:530–538PubMed

25.

Piazza O, Russo E, Cotena S, Esposito G, Tufano R (2007) Elevated S100B levels do not correlate with the severity of encephalopathy during sepsis. Br J Anaesth 99:518–521PubMedCrossRef

26.

Kress JP, Pohlman AS, O’Connor MF, Hall JB (2000) Daily interruption of sedative infusions in critically ill patients undergoing mechanical ventilation. N Engl J Med 342:1471–1477PubMedCrossRef

27.

Antonelli M, Azoulay E, Bonten M, Chastre J, Citerio G, Conti G, De Backer D, Lemaire F, Gerlach H, Groeneveld J, Hedenstierna G, Macrae D, Mancebo J, Maggiore SM, Mebazaa A, Metnitz P, Pugin J, Wernerman J, Zhang H (2009) Year in review in Intensive Care Medicine, 2008: I. Brain injury and neurology, renal failure and endocrinology, metabolism and nutrition, sepsis, infections and pneumonia. Intensive Care Med 35:30–44PubMedCrossRef

28.

Ebersoldt M, Sharshar T, Annane D (2007) Sepsis-associated delirium. Intensive Care Med 33:941–950PubMedCrossRef

29.

Messaris E, Memos N, Chatzigianni E (2004) Time-dependent mitochondrial mediated programmed neuronal cell death prolongs survival in sepsis. Crit Care Med 32:1764–1770PubMedCrossRef

30.

Darvishi K, Sharma S, Bhat AK, Bamezai RNK (2007) Mitochondrial DNA G10398A polymorphism imparts maternal haplogroup N a risk for breast and esophageal cancer. Cancer Lett 249:249–255PubMedCrossRef

31.

Weiss H, Friedrich T, Hofhaus G, Preis D (1991) The respiratory-chain NADH dehydrogenase (complex I) of mitochondria. Eur J Biochem 197:563–576PubMedCrossRef

32.

Amo T, Yadava N, Oh R, Nicholls DG, Brand MD (2008) Experimental assessment of bioenergetic differences caused by the common European mitochondrial DNA haplogroups H and T. Gene 411:69–76PubMedCrossRef

Title: Mitochondrial DNA haplogroup R in the Han population and recovery from septic encephalopathy
Authors: Yi Yang
Ping Zhang
Rong Lv
Qiang He
Yiling Zhu
Xianghong Yang
Jianghua Chen
Publication date: 01-10-2011
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 10/2011
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-011-2319-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Mitochondrial DNA haplogroup R in the Han population and recovery from septic encephalopathy

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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