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Clinical and Translational Allergy

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Open Access 01-12-2019 | Mites | Research

Evaluation of major mite allergens from European standardized commercial extracts for in vivo diagnosis: addressing the need for precision medicine

Authors: Ruperto González-Pérez, Paloma Poza-Guedes, Yvelise Barrios del Pino, Víctor Matheu, Inmaculada Sánchez-Machín

Published in: Clinical and Translational Allergy | Issue 1/2019

Abstract

Background

Skin prick testing is the first-line interventional method to diagnose IgE mediated allergic diseases. Methodological differences in manufacturing processes and extract standardization may lead to variations in the reagent quality and potency. The current study evaluates sixteen commercially available Dermatophagoides pteronyssinus and Blomia tropicalis extracts for allergy diagnosis from different European manufacturers regarding allergen composition and content and whether these differences could influence their biological activity.

Methods

Mite-allergic subjects (n = 21) were skin-tested with the extracts and studied for immunoglobulin E reactivity. Nine extracts from D. pteronyssinus and seven from B. tropicalis were analysed for total protein content by Bradford and ELISA double sandwich was used to quantify specific antibodies for D. pteronyssinus and B. tropicalis major allergens from nine different manufacturers.

Results

Mite extracts showed a 10–60 fold variation regarding the total protein content. The contents of the major allergens of D. pteronyssinus and B. tropicalis differed considerably (30–53 fold change) among the extracts. Blo t 5 was quantitatively present in < 50% of the of the B. tropicalis reagents and could not be clearly detected by immunoblotting in the majority of the B. tropicalis commercial extracts.

Conclusions

Certain natural D. pteronyssinus and B. tropicalis extracts lack important allergens showing a considerable variability in composition and content. A closer collaboration among clinicians, allergen manufacturing companies and regulatory agencies to improve the quality and consistency of D. pteronyssinus and B. tropicalis extracts is warranted to achieve a more precise diagnosis and treatment of house dust mite allergy.

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Title: Evaluation of major mite allergens from European standardized commercial extracts for in vivo diagnosis: addressing the need for precision medicine
Authors: Ruperto González-Pérez
Paloma Poza-Guedes
Yvelise Barrios del Pino
Víctor Matheu
Inmaculada Sánchez-Machín
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Mites
Published in: Clinical and Translational Allergy / Issue 1/2019
Electronic ISSN: 2045-7022
DOI: https://doi.org/10.1186/s13601-019-0254-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Evaluation of major mite allergens from European standardized commercial extracts for in vivo diagnosis: addressing the need for precision medicine

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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