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Archives of Gynecology and Obstetrics

Published in:

01-03-2020 | Miscarriage | Maternal-Fetal Medicine

H19 regulates angiogenic capacity of extravillous trophoblasts by H19/miR-106a-5p/VEGFA axis

Authors: Hong Zeng, Dongmei He, Hebin Xie, Yuhao Zhao, Zhaoqun Peng, Huan Deng, Jinyue Hu, Binyuan Jiang, Nenghui Liu

Published in: Archives of Gynecology and Obstetrics | Issue 3/2020

Abstract

Purpose

To investigate the role and underlying mechanism of H19 in regulating angiogenic capacity of extravillous trophoblasts.

Methods

Gain and loss of function experiments were performed using a human first-trimester extravillous trophoblast (EVT) cell line, HTR-8/SVneo cells. H19 was overexpressed or knocked down in HTR-8 cells by transfecting plasmid harboring whole-length H19 sequence (pH19) or siRNA specially targeting H19, respectively (siH19). Cell migration and tube-formation assay were assessed in the indicated groups. Gene expression was detected by RT-qPCR, Western blot, and ELISA assay.

Results

Overexpression of H19 in EVT cells increased cell migration and tube formation, while downregulation of H19 in EVT cells decreased cell migration and tube formation. Furthermore, we found that H19 played its role by VEGFA. In addition, we demonstrated the H19/miR-106a-5p/VEGFA regulatory axis in EVT. Experiments of the clinical specimen showed that H19 was very abundantly expressed in human first-trimester trophoblasts, and we found that the expression of H19 and VEGFA were significantly downregulated in the villous tissues from idiopathic recurrent miscarriage (RM) patients; moreover, the expression of H19 and VEGFA was positively correlated.

Conclusion

H19/miR-106a-5p/VEGFA axis plays a role in regulating the angiogenic capacity of EVT, which might contribute to idiopathic RM.

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Title: H19 regulates angiogenic capacity of extravillous trophoblasts by H19/miR-106a-5p/VEGFA axis
Authors: Hong Zeng
Dongmei He
Hebin Xie
Yuhao Zhao
Zhaoqun Peng
Huan Deng
Jinyue Hu
Binyuan Jiang
Nenghui Liu
Publication date: 01-03-2020
Publisher: Springer Berlin Heidelberg
Keyword: Miscarriage
Published in: Archives of Gynecology and Obstetrics / Issue 3/2020
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-020-05469-7

At a glance: The STEP trials

Springer Medicine

H19 regulates angiogenic capacity of extravillous trophoblasts by H19/miR-106a-5p/VEGFA axis

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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