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BMC Pregnancy and Childbirth

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Open Access 01-12-2024 | Miscarriage | Research

Effect of Day 3 cell number on the live birth rate of vitrified-warmed Day 5 single blastocyst transfer in young women

Authors: Pingping Qiu, Ronghui Ye, Ping Li, Hui Huang, Lu Ding

Published in: BMC Pregnancy and Childbirth | Issue 1/2024

Abstract

Background

Previous studies have reported inconsistent results regarding blastocyst selection with a high day 3 (D3) cell number and the eventual pregnancy outcomes. Thus, in this study, the relationship between the D3 cell number and clinical outcomes of day 5 single blastocyst transfer (SBT) in vitrified-warmed transfer cycles was investigated.

Methods

Our retrospective study included 1144 day 5 SBT in vitrified-warmed cycles between February 2016 and February 2021. All cycles were the first vitrified-warmed cycles, and the female patients were less than 35 years of age. Based on the D3 cell number, the cycles were divided into four groups, as follows: group A (3–7 cells, n = 130); group B (8–9 cells, n = 621); group C (10–12 cells, n = 328); and group D (13–16 cells, n = 65). The differences in the live birth rate (LBR), clinical pregnancy rate, and miscarriage rate were examined among the four groups.

Results

The LBR and clinical pregnancy rate increased with the D3 cell number (P < 0.01). No significant difference was found in the miscarriage rate among the groups (P = 0.055). After adjusting for confounding factors, the LBR was significantly higher in groups C (odds ratio [OR] = 1.477, 95% confidence interval [CI]: 1.124–1.941, P = 0.005) and D (OR = 2.000, 95% CI: 1.166–3.429, P = 0.012) than in group B.

Conclusions

A high D3 cell number (> 9 cells) was associated with a high LBR in the vitrified-warmed day 5 SBT cycles of patients < 35 years of age. The cell number of D3 embryos can be an important reference indicator for blastocyst selection. Among blastocysts with the same morphological score, those with > 9 cells on D3 can be preferentially selected for transplantation.

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Title: Effect of Day 3 cell number on the live birth rate of vitrified-warmed Day 5 single blastocyst transfer in young women
Authors: Pingping Qiu
Ronghui Ye
Ping Li
Hui Huang
Lu Ding
Publication date: 01-12-2024
Publisher: BioMed Central
Keyword: Miscarriage
Published in: BMC Pregnancy and Childbirth / Issue 1/2024
Electronic ISSN: 1471-2393
DOI: https://doi.org/10.1186/s12884-024-06468-1

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effect of Day 3 cell number on the live birth rate of vitrified-warmed Day 5 single blastocyst transfer in young women

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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