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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 8/2013

01-08-2013 | Embryo Biology

The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study

Authors: Esti Kasterstein, Deborah Strassburger, Daphna Komarovsky, Orna Bern, Alisa Komsky, Arieh Raziel, Shevach Friedler, Raphael Ron-El

Published in: Journal of Assisted Reproduction and Genetics | Issue 8/2013

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Abstract

Purpose

This prospective randomized study used sibling oocytes of 258 women with ≥8 oocytes to compare the effect of 5 % O2 versus 20 % O2 concentrations on embryo development and clinical outcome.

Methods

Oocytes of each case were divided between incubators with either 5 % or 20 % O2 concentration. Outcome measures were fertilization, cleavage, embryo quality, blastocyst formation, and implantation, pregnancy and live birth rates.

Results

Fertilization and cleavage rates were similar in both groups. The 5 % O2 group had significantly more blastomeres (P < 0.05) and more top-quality embryos on day 3 (P < 0.02), as well as significantly more available embryos for transfer (31.6 % vs. 23.1 % for the 20 % O2 group; P < 0.0001). There were significantly more cycles with good embryos in the 5 % group (76/258) than in the 20 % group (38/258) (P < 0.0001). Implantation and pregnancy rates were significantly higher for 5 % O2 embryos (P < 0.03 and P < 0.05, respectively). Live birth rates per embryo transfer were 34.2 % and 15.8 %, respectively, P < 0.05.

Conclusions

Implantation, pregnancy and live birth rates are higher, and more good quality embryos are available for transfer and freezing with reduced rather than with atmospheric oxygen concentrations during embryo incubation.
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Metadata
Title
The effect of two distinct levels of oxygen concentration on embryo development in a sibling oocyte study
Authors
Esti Kasterstein
Deborah Strassburger
Daphna Komarovsky
Orna Bern
Alisa Komsky
Arieh Raziel
Shevach Friedler
Raphael Ron-El
Publication date
01-08-2013
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 8/2013
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-013-0032-z

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