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Journal of Cardiothoracic Surgery

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Open Access 01-12-2021 | Research article

MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

Authors: Jiangwei Hu, Xinqin Xiang, Wei Guan, Weihua Lou, Junming He, Jian Chen, Yin Fu, Guoliang Lou

Published in: Journal of Cardiothoracic Surgery | Issue 1/2021

Abstract

Background

So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC).

Methods

MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis.

Results

Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4.

Conclusion

By targeting CDCA4, miR-497-5p restrained development of LUSC.

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Title: MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression
Authors: Jiangwei Hu
Xinqin Xiang
Wei Guan
Weihua Lou
Junming He
Jian Chen
Yin Fu
Guoliang Lou
Publication date: 01-12-2021
Publisher: BioMed Central
Published in: Journal of Cardiothoracic Surgery / Issue 1/2021
Electronic ISSN: 1749-8090
DOI: https://doi.org/10.1186/s13019-021-01698-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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