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Open Access 01-12-2022 | Migraine | Research

Repeated inflammatory dural stimulation-induced cephalic allodynia causes alteration of gut microbial composition in rats

Authors: Shuai Miao, Wenjing Tang, Heng Li, Bozhi Li, Chunxiao Yang, Wei Xie, Tao Wang, Wenhao Bai, Zihua Gong, Zhao Dong, Shengyuan Yu

Published in: The Journal of Headache and Pain | Issue 1/2022

Abstract

Background

Gut microbial dysbiosis and gut-brain axis dysfunction have been implicated in the pathophysiology of migraine. However, it is unclear whether migraine-related cephalic allodynia could induce the alteration of gut microbial composition.

Methods

A classic migraine rat model was established by repeated dural infusions of inflammatory soup (IS). Periorbital mechanical threshold and nociception-related behaviors were used to evaluate IS-induced cephalic allodynia and the preventive effect of topiramate. The alterations in gut microbial composition and potential metabolic pathways were investigated based on the results of 16 S rRNA gene sequencing. Microbiota-related short-chain fatty acids and tryptophan metabolites were detected and quantified by mass spectrometry analysis.

Results

Repeated dural IS infusions induced cephalic allodynia (decreased mechanical threshold), migraine-like behaviors (increased immobility time and reduced moving distance), and microbial composition alteration, which were ameliorated by the treatment of topiramate. Decreased Lactobacillus was the most prominent biomarker genus in the IS-induced alteration of microbial composition. Additionally, IS infusions also enhanced metabolic pathways of the gut microbiota in butanoate, propanoate, and tryptophan, while the increased tryptophan-related metabolites indole-3-acetamide and tryptophol in feces could be the indicators.

Conclusions

Inflammatory dural stimulation-induced cephalic allodynia causes the alterations of gut microbiota profile and microbial metabolic pathways.

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Title: Repeated inflammatory dural stimulation-induced cephalic allodynia causes alteration of gut microbial composition in rats
Authors: Shuai Miao
Wenjing Tang
Heng Li
Bozhi Li
Chunxiao Yang
Wei Xie
Tao Wang
Wenhao Bai
Zihua Gong
Zhao Dong
Shengyuan Yu
Publication date: 01-12-2022
Publisher: Springer Milan
Keywords: Migraine
Respiratory Microbiota
Topiramate
Published in: The Journal of Headache and Pain / Issue 1/2022
Print ISSN: 1129-2369
Electronic ISSN: 1129-2377
DOI: https://doi.org/10.1186/s10194-022-01441-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Repeated inflammatory dural stimulation-induced cephalic allodynia causes alteration of gut microbial composition in rats

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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