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BMC Cancer

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Open Access 01-12-2012 | Research article

MicroRNA-143 down-regulates Hexokinase 2 in colon cancer cells

Authors: Lea H Gregersen, Anders Jacobsen, Lisa B Frankel, Jiayu Wen, Anders Krogh, Anders H Lund

Published in: BMC Cancer | Issue 1/2012

Abstract

Background

MicroRNAs (miRNAs) are well recognized as gene regulators and have been implicated in the regulation of development as well as human diseases. miR-143 is located at a fragile site on chromosome 5 frequently deleted in cancer, and has been reported to be down-regulated in several cancers including colon cancer.

Methods

To gain insight into the role of miR-143 in colon cancer, we used a microarray-based approach in combination with seed site enrichment analysis to identify miR-143 targets.

Results

As expected, transcripts down-regulated upon miR-143 overexpression had a significant enrichment of miR-143 seed sites in their 3'UTRs. Here we report the identification of Hexokinase 2 (HK2) as a direct target of miR-143. We show that re-introduction of miR-143 in the colon cancer cell line DLD-1 results in a decreased lactate secretion.

Conclusion

We have identified and validated HK2 as a miR-143 target. Furthermore, our results indicate that miR-143 mediated down-regulation of HK2 affects glucose metabolism in colon cancer cells. We hypothesize that loss of miR-143-mediated repression of HK2 can promote glucose metabolism in cancer cells, contributing to the shift towards aerobic glycolysis observed in many tumors.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/12/232/prepub

Title: MicroRNA-143 down-regulates Hexokinase 2 in colon cancer cells
Authors: Lea H Gregersen
Anders Jacobsen
Lisa B Frankel
Jiayu Wen
Anders Krogh
Anders H Lund
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: BMC Cancer / Issue 1/2012
Electronic ISSN: 1471-2407
DOI: https://doi.org/10.1186/1471-2407-12-232

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