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Clinical Oral Investigations

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01-03-2020 | Metoprolol | Original Article

The effects of intraperitoneal metoprolol administration on healing of bone defects in rat tibia: a pilot study

Authors: R. Al Alawy, H. Hammad, R. AlHabashneh

Published in: Clinical Oral Investigations | Issue 3/2020

Abstract

Objectives

Metoprolol is a cardioselective competitive beta-1 adrenergic receptor antagonist with antihypertensive properties, devoid of intrinsic sympathomimetic activity. Various studies have suggested the effect of beta-blockers on bone remodeling. We aimed to investigate whether metoprolol affects bone remodeling by altering anti-inflammatory and pro-inflammatory cytokines.

Materials and methods

Surgical defects of 3 mm diameter were created in tibiae of 72 Sprague-Dawley rats. Rats were randomly assigned to a control group without metoprolol treatment (n = 36), and a test group treated with 0.1 mg/kg/day metoprolol (n = 36). Six rats from each group were sacrificed at days 0, 1, 3, 5, 7, and 14. The percentages of cells, which showed positive immunohistochemical staining for IL-1β, IL-6, IL-10, and RANKL, were assessed in the defect area. Differences in percentages of stained cells within each of the test and control groups over various time intervals were tested using one-way ANOVA test. A P value of < 0.05 was considered statistically significant.

Results

No significant differences in IL-1β, IL-10, IL-6, and RANKL expressions were found between test and control groups at the same interval. Significant reduction was observed at different time intervals in the same group (P < 0.05).

Conclusion

Metoprolol did not reduce bone-active cytokine: IL-1β, IL-6, and RANKL. It also did not elevate IL-10 expression levels. Thus, it does not appear to decrease osteoclastogenesis.

Clinical relevance

Results from this animal model help us understand any effect of metoprolol on bone healing by potential contribution to different real-world clinical research.

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Title: The effects of intraperitoneal metoprolol administration on healing of bone defects in rat tibia: a pilot study
Authors: R. Al Alawy
H. Hammad
R. AlHabashneh
Publication date: 01-03-2020
Publisher: Springer Berlin Heidelberg
Keywords: Metoprolol
Bone Defect
Cytokines
Published in: Clinical Oral Investigations / Issue 3/2020
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-02987-w

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The effects of intraperitoneal metoprolol administration on healing of bone defects in rat tibia: a pilot study

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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