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Archives of Virology
Published in: Archives of Virology 5/2020

01-05-2020 | Annotated Sequence Record

Metaviromic analysis reveals coinfection of papaya in western Kenya with a unique strain of Moroccan watermelon mosaic virus and a novel member of the family Alphaflexiviridae

Authors: David Alan Read, John Muoma, Genevieve Dawn Thompson

Published in: Archives of Virology | Issue 5/2020

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Abstract

Severe mottling symptoms were observed on Carica papaya L. in Koyonzo, Kakamega County, Kenya. Total RNA was sequenced via an RNAtag-seq workflow. Assembled contigs indicated the presence of a divergent strain of Moroccan watermelon mosaic virus (genus Potyvirus) with a complete genome length of 9,733 nt (GenBank accession no. MN418119). Additionally, the complete genome sequence of a novel member of the viral genus Allexivirus was determined (GenBank accession no. MN418120). The genome contains six open reading frames (ORFs) that show varying degrees of sequence similarity to members of the genus Allexivirus; however, it appears to lack an ORF encoding a nucleic-acid-binding homolog. The tentative name “papaya virus A” (PaVA) has been proposed for this virus.
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Metadata
Title
Metaviromic analysis reveals coinfection of papaya in western Kenya with a unique strain of Moroccan watermelon mosaic virus and a novel member of the family Alphaflexiviridae
Authors
David Alan Read
John Muoma
Genevieve Dawn Thompson
Publication date
01-05-2020
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 5/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-020-04582-x

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