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01-06-2019 | Melanoma | Original Paper

Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels

Authors: Weicheng Wang, Souichi Nakashima, Seikou Nakamura, Yoshimi Oda, Hisashi Matsuda

Published in: Journal of Natural Medicines | Issue 3/2019

Abstract

Auriculataoside A, an anthracenone dimer glycoside isolated from Cassia auriculata seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC₅₀ value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action underlying the anti-proliferative effect of auriculataoside A on cells, we examined changes in whole protein expression after treatment with auriculataoside A and found that the expression Cdc42, RhoA, and Rac1, which are Rho family GTPases, was reduced. Auriculataoside A also arrested the cell cycle at G1 phase. These results suggest that the suppression of the above proteins induced G1 arrest. In addition, auriculataoside A also suppressed the expression of β-catenin and c-Myc proteins. This action of auriculataoside A could be one of the mechanisms underlying its selective anti-proliferative effect on B16 melanoma cells.

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Title: Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels
Authors: Weicheng Wang
Souichi Nakashima
Seikou Nakamura
Yoshimi Oda
Hisashi Matsuda
Publication date: 01-06-2019
Publisher: Springer Singapore
Keywords: Melanoma
Melanoma
Published in: Journal of Natural Medicines / Issue 3/2019
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-018-01278-0

At a glance: The STEP trials

Springer Medicine

Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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