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Journal of Inflammation
Published in: Journal of Inflammation 1/2024

Open Access 01-12-2024 | Mastitis | Research

The preventive effects of Lactobacillus casei 03 on Escherichia coli-induced mastitis in vitro and in vivo

Authors: Ke Li, Ming Yang, Mengyue Tian, Li Jia, Yinghao Wu, Jinliang Du, Lining Yuan, Lianmin Li, Yuzhong Ma

Published in: Journal of Inflammation | Issue 1/2024

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Abstract

Background

Lactobacillus casei possesses many kinds of bioactivities, such as anti-inflammation and anti-oxidant, and has been applied to treating multiple inflammatory diseases. However, its role in mastitis prevention has remained ambiguous.

Methods

This study aimed to examine the mechanisms underlying the preventive effects of L. casei 03 against E. coli- mastitis utilizing bovine mammary epithelial cells (BMECs) and a mouse model.

Results

In vitro assays revealed pretreatment with L. casei 03 reduced the apoptotic ratio and the mRNA expression levels of IL1β, IL6 and TNFα and suppressed phosphorylation of p65, IκBα, p38, JNK and ERK in the NF-κB signaling pathway and MAPK signaling pathway. Furthermore, in vivo tests indicated that intramammary infusion of L. casei 03 relieved pathological changes, reduced the secretion of IL1β, IL6 and TNFα and MPO activity in the mouse mastitis model.

Conclusions

These data suggest that L. casei 03 exerts protective effects against E. coli-induced mastitis in vitro and in vivo and may hold promise as a novel agent for the prevention and treatment of mastitis.
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Metadata
Title
The preventive effects of Lactobacillus casei 03 on Escherichia coli-induced mastitis in vitro and in vivo
Authors
Ke Li
Ming Yang
Mengyue Tian
Li Jia
Yinghao Wu
Jinliang Du
Lining Yuan
Lianmin Li
Yuzhong Ma
Publication date
01-12-2024
Publisher
BioMed Central
Keyword
Mastitis
Published in
Journal of Inflammation / Issue 1/2024
Electronic ISSN: 1476-9255
DOI
https://doi.org/10.1186/s12950-024-00378-x

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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