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Archives of Virology
Published in: Archives of Virology 11/2010

01-11-2010 | Original Article

Marek’s disease virus microRNA designated Mdv1-pre-miR-M4 targets both cellular and viral genes

Authors: Benoît Muylkens, Damien Coupeau, Ginette Dambrine, Sascha Trapp, Denis Rasschaert

Published in: Archives of Virology | Issue 11/2010

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Abstract

Mdv1-miR-M4 is one of 25 microRNAs (miRNAs) expressed by Marek’s disease virus (MDV-1), an oncogenic alphaherpesvirus that induces fatal T-cell lymphoma in chickens. Mdv1-miR-M4 was shown to be the second functional viral ortholog of miR-155, a cellular miRNA that plays a crucial role in several physiological and pathological processes in lymphocyte biology. In this study, we investigated a panel of putative mdv1-miR-M4 targets involved in gene networks affecting both cellular and viral life cycles. Using luciferase reporter assays, we showed that mdv1-miR-M4-5P and miR-155 efficiently targeted a common set of 3′ untranslated regions (3′UTR) of six cellular genes (GPM6B, RREB1, c-Myb, MAP3K7IP2, PU.1 and C/EBP). In addition, we also investigated the interactions between mdv1-miR-M4-5P and mdv1-miR-M43P and viral mRNAs encoding UL28 and UL32 in both reporter and western blot assays. Mdv1-miR-M4 specifically inhibited the translation of these two viral proteins, which are involved in the cleavage/packaging of herpesvirus DNA.
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Metadata
Title
Marek’s disease virus microRNA designated Mdv1-pre-miR-M4 targets both cellular and viral genes
Authors
Benoît Muylkens
Damien Coupeau
Ginette Dambrine
Sascha Trapp
Denis Rasschaert
Publication date
01-11-2010
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2010
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-010-0777-y

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