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Lasers in Medical Science
Published in: Lasers in Medical Science 5/2014

01-09-2014 | Original Article

Low-level laser therapy prevents degenerative morphological changes in an experimental model of anterior cruciate ligament transection in rats

Authors: Caroline Bublitz, Carla Medalha, Poliani Oliveira, Lívia Assis, Luiz Paulo Milares, Kelly Rossetti Fernandes, Carla Roberta Tim, Fernando Augusto Vasilceac, Stela Marcia Mattiello, Ana Claudia Muniz Renno

Published in: Lasers in Medical Science | Issue 5/2014

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Abstract

The aim of this study was to analyze the effects of low-level laser therapy (LLLT) on the prevention of cartilage damage after the anterior cruciate ligament transection (ACLT) in knees of rats. Thirty male rats (Wistar) were distributed into three groups (n = 10 each): injured control group (CG); injured laser-treated group at 10 J/cm2 (L10), and injured laser-treated group at 50 J/cm2 (L50). Laser treatment started immediately after the surgery and it was performed for 15 sessions. An 808 nm laser, at 10 and 50 J/cm2, was used. To evaluate the effects of LLLT, the qualitative and semi-quantitative histological, morphometric, and immunohistochemistry analysis were performed. Initial signs of tissue degradation were observed in CG. Interestingly, laser-treated animals presented a better tissue organization, especially at the fluence of 10 J/cm2. Furthermore, laser phototherapy was able of modulating some of the aspects related to the degenerative process, such as the prevention of proteoglycans loss and the increase in cartilage area. However, LLLT was not able of modulating chondrocytes proliferation and the immunoexpression of markers related to inflammatory process (IL-1 and MMP-13). This study showed that 808 nm laser, at both fluences, prevented features related to the articular degenerative process in the knees of rats after ACLT.
Literature
1.
Lin HD, He CQ, Luo QL, Zhang JL, Zeng DX (2012) The effect of low-level to apoptosis of chondrocytes and caspase expression, including caspase-8 e caspase-3 in rabbit surgery-induced model of knee. Rheumatol Int 32:759–766PubMedCrossRef
2.
Jin SY, Hong SJ, Yang HI, Park SD, Yoo MC, Lee HJ, Hong MS, Park HJ, Yoon SH, Kim BS, Yim SV, Park HK, Chung JH (2004) Estrogen receptor-alpha gene haplotype is associated with primary knee osteoarthritis in Korean population. Arthritis Res Ther 6:R415–R421PubMedCentralPubMedCrossRef
3.
Gupta S, Hawker GA, Laporte A, Croxford R, Coyte PC (2005) The economic burden of disabling hip and knee osteoarthritis (OA) from the perspective of individuals living with this condition. Rheumatology 44:1531–1537PubMedCrossRef
4.
Goldring MB (2000) Osteoarthritis and cartilage: the role of cytokines. Curr Rheumatol Rep 2:459–465PubMedCrossRef
5.
Pereira D, Peleteiro B, Araújo J, Branco J, Santos RA, Ramos E (2011) The effect of osteoarthritis definition on prevalence and incidence estimates: a systematic review. Osteoarthr Cartil 19:1270–1285PubMedCrossRef
6.
7.
Steinhilber B, Haupt G, Boeer J, Grau S, Krauss I (2011) Reproducibility of concentric isokinetic and isometric strength measurements at the hip in patients with hip osteoarthritis: a preliminary study. Isokinet Exerc Sci 19:39–46
8.
9.
Kushibiki T, Tajiri T, Ninomiya Y, Awazu K (2010) Chondrogenic mRNA expression in prechondrogenic cells after blue laser irradiation. J Photochem Photobiol B 98:211–215PubMedCrossRef
10.
Wong BJ, Pandhoh N, Truong MT, Diaz S, Chao K, Hou S, Gardiner D (2005) Identification of chondrocyte proliferation following laser irradiation, thermal injury, and mechanical trauma. Lasers Surg Med 37:89–96PubMedCrossRef
11.
Karu TI, Kolyakov SF (2005) Exact action spectra for cellular response relevant to phototherapy. Photomed Laser Surg 23:355–361PubMedCrossRef
12.
Prindeze NJ, Moffatt LT, Shupp JW (2012) Mechanisms of action for light therapy: a review of molecular interactions. Exp Biol Med (Maywood) 237:1241–1248CrossRef
13.
Castano AP, Dai TH, Yaroslavsky I, Cohen R, Apruzzese WA, Smotrich MH, Hamblin MR (2007) Low-level laser therapy for zymosan-induced arthritis in rats: importance of illumination time. Lasers Surg Med 39:543–550PubMedCentralPubMedCrossRef
14.
Cho JH, Lim SC, Kim SG, Kim YS, Kang SS, Choi SH, Cho YS, Bae CS (2004) Effect of low-level laser therapy on osteoarthropathy in rabbit. In Vivo 18:585–592PubMed
15.
da Rosa AS, dos Santos AF, da Silva MM, Facco GG, Perreira DM, Alves AC, Leal Junior EC, de Carvalho PT (2012) Effects of low-level laser therapy at wavelengths of 660 and 808 nm in experimental model of osteoarthritis. Photochem Photobiol 88:161–166PubMedCrossRef
16.
Delfino GB, Peviani SM, Durigan JL, Russo TL, Baptista IL, Ferretti M, Moriscot AS, Salvini TF (2013) Quadriceps muscle atrophy after anterior cruciate ligament transection involves increased mRNA levels of atrogin-1, muscle ring finger 1, and myostatin. Am J Phys Med Rehabil 92:411–419PubMedCrossRef
17.
Medalha CC, Di Gangi GC, Barbosa CB, Fernandes M, Aguiar O, Faloppa F, Leite VM, Renno AC (2012) Low-level laser therapy improves repair following complete resection of the sciatic nerve in rats. Lasers Med Sci 27(3):629–635PubMedCrossRef
18.
Renner AF, Carvalho E, Soares E, Mattiello-Rosa S (2006) The effect of a passive muscle stretching protocol on the articular cartilage. Osteoarthr Cartil 14:196–202PubMedCrossRef
19.
Pedrosa WF Jr, Okamoto R, Faria PE, Arnez MF, Xavier SP, Salata LA (2009) Immunohistochemical, tomographic and histological study on onlay bone graft remodeling. Part II: calvarial bone. Clin Oral Implants Res 20:1254–1264PubMedCrossRef
20.
Tim CR, Pinto KN, Rossi BR, Fernandes K, Matsumoto MA, Parizotto NA, Rennó AC (2013) Low-level laser therapy enhances the expression of osteogenic factors during bone repair in rats. Lasers Med Sci. [Epub ahead of print]
21.
Alfredo PP, Bjordal JM, Dreyer SH, Meneses SR, Zaguetti G, Ovanessian V, Fukuda TY, Junior WS, Lopes Martins RÁ, Casarotto RA, Marques AP (2012) Efficacy of low level laser therapy associated with exercises in knee osteoarthritis: a randomized double-blind study. Clin Rehabil 26:523–533PubMedCrossRef
22.
Alves ACA, Carvalho PTC, Parente M, Xavier M, Frigo L, Aimbire F, Leal-Junior ECP, Albertini R (2013) Low-level laser therapy in different stages of rheumatoid arthritis: a histological study. Lasers Med Sci 28:529–536PubMedCrossRef
23.
Pallotta RC, Bjordal JM, Frigo L, Leal- Junior ECP, Teixeira S, Marcos RL, Ramos L, Messias FM, Lopes-Martins RAB (2012) Infrared (810-nm) low-level laser therapy on rat experimental knee inflammation. Lasers Med Sci 27:71–78PubMedCentralPubMedCrossRef
24.
Lane NE, Brandt K, Hawker G, Peeva E, Schreyer E, Tsuji W, Hochberg MC (2011) OARSI-FDA initiative: defining the disease state of osteoarthritis. Osteoarthr Cartil 19:478–482PubMedCrossRef
25.
Ding C, Jones G, Wluka AE, Cicuttini F (2010) What can we learn about osteoarthritis by studying a healthy person against a person with early onset of disease? Curr Opin Rheumatol 22:520–527PubMedCrossRef
26.
27.
Fujita I, Hirata S, Ishikawa H, Mizuno K, Itoh H (1997) Apoptosis of hypertrophic chondrocytes in rat cartilaginous growth plate. J Orthop Sci 2:328–333CrossRef
28.
Kuhnt K, D’ Lima DD, Hashimoto S, Lotz M (2004) Review—cell death in cartilage. Osteoarthr Cartil 12:1–16
29.
Caterson B, Flannery CR, Hughes CE, Little CB (2000) Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333–344PubMedCrossRef
30.
Narmoneva DA, Cheung HS, Wang JY, Howell DS, Setton LA (2002) Altered swelling behavior of femoral cartilage following joint immobilization in canine model. J Orthop Res 20:83–91PubMedCrossRef
31.
Gottlieb T, Jorgensen B, Rohde E, Müller G, Scheller EE (2006) The influence of irradiation low-level diode laser on the proteoglycan content in arthrotically cartilage in rabbits. Med Laser Appl 21:53–59CrossRef
32.
Soriano F, Campana V, Moya M, Gavotto A, Simes J, Soriano M, Soriano R, Spitale L, Palma J (2006) Photobiomodulation of pain and inflammation in microcrystalline arthropathies: experimental and clinical results. Photomed Laser Surg 24:140–150PubMedCrossRef
33.
34.
Hasegawa A, Nakahara H, Kinoshita M, Asahara H, Koziol J, Lotz MK (2013) Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis. Arthritis Res Ther 15(1):R29, Epub ahead of printPubMedCentralPubMedCrossRef
35.
Guo H, Luo Q, Zhang J, Lin H, Xia L, He C (2011) Comparing different physical factors on serum TNF-α levels, chondrocyte apoptosis, caspase-3 and caspase-8 expression in osteoarthritis of the knee in rabbits. Joint Bone Spine 78:604–610PubMedCrossRef
36.
Renno ACM, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25:275–280PubMedCrossRef
37.
Moore P, Ridgway TD, Higbee RG, Howard EW, Lucroy MD (2005) Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro. Lasers Surg Med 1:8–12CrossRef
38.
Kujawa J, Zavodnik L, Zavodnik I, Buko V, Lapshyna A, Bryszewska M (2004) Effect of low-intensity (3.75–25 J/cm2) near-infrared (810 nm) laser radiation on red blood cell ATPase activities and membrane structure. J Clin Laser Med Surg 22:111–117PubMedCrossRef
Metadata
Title
Low-level laser therapy prevents degenerative morphological changes in an experimental model of anterior cruciate ligament transection in rats
Authors
Caroline Bublitz
Carla Medalha
Poliani Oliveira
Lívia Assis
Luiz Paulo Milares
Kelly Rossetti Fernandes
Carla Roberta Tim
Fernando Augusto Vasilceac
Stela Marcia Mattiello
Ana Claudia Muniz Renno
Publication date
01-09-2014
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 5/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-014-1546-z

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