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01-03-2012 | Original Article

The effect of low-level laser to apoptosis of chondrocyte and caspases expression, including caspase-8 and caspase-3 in rabbit surgery-induced model of knee osteoarthritis

Authors: Hai-Dan Lin, Cheng-Qi He, Qing-Lu Luo, Jing-Long Zhang, De-Xin Zeng

Published in: Rheumatology International | Issue 3/2012

Abstract

The purpose of this study was to observe the effect of 810-nm low-level laser to apoptosis of chondrocyte and related proteins, including caspase-3 and caspase-8, in rabbit surgery-induced model of knee osteoarthritis. A total of 24 New Zealand White rabbits were randomly assigned into 3 groups: test group, model group, and normal group. The rabbits in test group and model group received anterior cruciate ligament transection in the right knee. Six weeks after transection, the rabbits in test group were given 10-session 810-nm laser illumination. Eight weeks after transection, all animal were killed. Modified Mankin Score was made for histological assessment. The caspases expressions and chondrocytes apoptosis were tested using the immunohistochemistry and TUNEL assessment, respectively. The modified Mankin Score of test group was significantly lower than model group (P < 0.01) and higher than normal group (P < 0.01). The caspase-8 expression of test group was lower than model group and higher than normal group, but no significant difference was found (P > 0.05). This study revealed that the 810-nm low-level laser can improve cartilage structure, prevent articular cartilage degradation and significantly decrease the expression of caspase-3 in this surgery-induced OA model. Further studies are needed.

Loza E, Lopez-Gomez JM, Abasolo L, Maese J, Carmon L, Batlle-Gualda E (2009) Economic burden of knee and hip osteoarthritis in Spain. Arthritis Rheum 61:158–165PubMedCrossRef

Gupta S, Hawker GA, Laporte A, Croxford R, Coyte PC (2005) The economic burden of disabling hip and knee osteoarthritis (OA) from the perspective of individuals living with this condition. Rheumatology 44:1531–1537PubMedCrossRef

D’Lima DD, Hashimoto S, Chen PC, Colwell CW, Lotz MK (2001) Human chondrocyte apoptosis in response to mechanical injury. Osteoarthritis Cartilage 9:712–719

Aigner T, Kim HA (2002) Apoptosis and cellular vitality: issues in osteoarthritic cartilage degradation. Arthritis Rheum 46:1986–1996PubMedCrossRef

Heraud F, Heraud A, Harmand MF (2000) Apoptosis in normal and osteoarthritic human articular cartilage. Ann Rheum Dis 59:959–965PubMedCrossRef

Kim HA, Lee YJ, Seong SC, Choe KW, Song YW (2000) Apoptotic chondrocyte death in human osteoarthritis. J Rheumatol 27:455–462PubMed

Sharif M, Whitehouse A, Sharman P, Perry M, Adams M (2004) Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3. Arthritis Rheum 50:507–515PubMedCrossRef

Robertson CM, Pennock AT, Harwood FL, Pomerleau AC, Allen RT, Amiel D (2006) Characterization of pro-apoptotic and matrix-degradative gene expression following induction of osteoarthritis in mature and aged rabbits. Osteoarthritis Cartilage 14:471–476PubMedCrossRef

Soriano F, Campana V, Moya M, Gavotto A, Simes J, Soriano M, Soriano R, Spitale L, Palma J (2006) Photobiomodulation of pain and inflammation in microcrystalline arthropathies: experimental and clinical results. Photomed Laser Surg 24:140–150PubMedCrossRef

10.

Stergioulas A (2004) Low-level laser treatment can reduce edema in second degree ankle sprains. J Clin Laser Med Surg 22:125–128PubMedCrossRef

11.

Gur A, Sarac AJ, Cevik R, Altindag O, Sarac S (2004) Efficacy of 904nm gallium arsenide low level laser therapy in the management of chronic myofascial pain in the neck: a double-blind and randomize-controlled trial. Lasers Surg Med 35:229–235PubMedCrossRef

12.

Ozdemir F, Birtane M, Kokino S (2001) The Clinical Efficacy of Low-Power Laser Therapy on Pain and Function in Cervical Osteoarthritis. Clin Rheumatol 20:181–184

13.

Yurtkuran M, Konur S, Ozcakir S, Bingol U (2007) Laser acupuncture in knee osteoarthritis. A double-blind randomized controlled study. Photomed Laser Surg 25:14–20PubMedCrossRef

14.

Gur A, Cosut A, Sarac AJ, Cevik R, Nas K, Uyar A (2003) Efficacy of Different Therapy Regimes of Low-Power Laser in Painful Osteoarthritis of the Knee: A Double-Blind and Randomized-Controlled Trial[J]. Lasers Surg Med 33:330–338

15.

Castano AP, Dai TH, Yaroslavsky I, Cohen R, Apruzzese WA, Smotrich MH, Hamblin MR (2007) Low-level laser therapy for zymosan-induced arthritis in rats: importance of illumination time. Lasers Surg Med 39:543–550PubMedCrossRef

16.

Guerino MR, Baranauskas V, Guerino AC, Parizotto N (2000) Laser treatment of experimentally-induced chronic arthritis. Appl Surf Sci 154:561–564

17.

Cho HJ, Lim SC, Kim SG, Kim YS, Kang SS, Choi SH, Cho YS, Bae CS (2004) Effect of ow-level laser therapy on osteoarthropathy in rabbit. In Vivo 18:585–591

18.

Bayat M, Ansari E, Gholami N, Bayat A (2007) Effect of low-level helium–neon laser therapy on histological and ultrastructural features of immobilized rabbit articular cartilage. J Photochem Photobiol B 87:81–87PubMedCrossRef

19.

Bulow PM, Jensen H, Danneskiold-Samsoe B (1994) Low power Ga-Al-As laser treatment of painful osteoarthritis of the knee. A double-blind placebo-controlled study. Scand J Rehabil Med 26:155–159PubMed

20.

Tascioglu F, Armagan O, Tabak Y, Corapci I, Oner C (2004) Low power laser treatment in patients with knee osteoarthritis. Swiss Med Wkly 134:254–258

21.

Lucie B, George W, Serge M, Gaboury I, Stokes B, Morin M, Casimiro L, Yonge K, Tugwell P (2005) Randomized Controlled Trial on Low Level Laser Therapy (LLLT) in the Treatment of Osteoarthritis (OA) of the Hand.Lasers. Surg Med 36:210–219

22.

Robert D (2008) Animal models for arthritis. Best Pract Res Clin Rheumatol 22:253–267

23.

Pennock AT, Robertson CM, Emmerson BC, Harwood FL, Amiel D (2007) Role of apoptotic and matrix-degrading genes in articular cartilage and meniscus of mature and aged rabbits during development of osteoarthritis. Arthritis Rheum 56:1529–1536PubMedCrossRef

24.

Sah RL, Yang AS, Chen AC, Hant JJ, Halili RB, Yoshioka M, Amiel D, Coutts RD (1997) Physical properties of rabbit articular cartilage after transection of the anterior cruciate ligament. J Orthop Res 15:197–203PubMedCrossRef

25.

Van der Sluijs JA, Geesink RG, van der Linden AJ, Bulstra SK, Kuyer R, Drukker J (1992) The reliability of the Mankin score for osteoarthritis. J Orthop Res 10:58–61PubMedCrossRef

26.

Karu TI, Pyatibrat LV, Afanasyeva NI (2004) A novel mitochondrial signaling pathway activated by visible-to-near infrared radiation. Photochem photobiol 2:366–372

27.

Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 1:1–17CrossRef

28.

Passarella S, Casamassima E, Molinari S, Pastore D, Quagliariello E, Catalano IM, Cingolani A (1984) Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium-neon laser. Fed Eur Biochem Soc Lett 1:95–99CrossRef

29.

Blanco FJ, Guitian R, Vazquez-Martul E, de Toro FJ, Galdo F (1998) Osteoarthrtitic chondrocytes die by apoptosis: a possible pathway for osteoarthritis pathology. Arthritis Rheum 41(27):284–289

30.

Nicholson DW (1999) Caspase structure, proteolytic substrates, and function during apoptotic cell death. Cell Death Differ 6(28):1028–1042

31.

Heyninck K, Beyaert R (2001) Crosstalk between NF-jB-activating and apoptosis-inducing proteins of the TNF-receptor complex. Mol Cell Biol Res Commun 4(29):259–265

32.

Mak TW, Yeh WC (2002) Signaling for survival and apoptosis in the immune system. Arthritis Res 4:S243–252

33.

Whittaker P (2004) Laser acupuncture: past, present, and future. Lasers Med Sci 19:69–80PubMedCrossRef

34.

Ackermann G, Hartmann M, Scherer K, Lang EW, Hohenleutner U, Landthaler M, Bäumler W (2002) Correlations between light penetration into skin and the therapeutic outcome following laser therapy of port-wine stains. Lasers Med Sci 17:70–78PubMedCrossRef

35.

Papaioannou NA, Krallis N, Triantafillopoulos IK, Khaldi L, Dontas I, Lyritis GP (2004) Optimal timing of research after anterior cruciate ligament resection in rabbits. Contemp Top Lab Anim Sci 43(26):22–27

36.

Wu SN, Xing D, Wang F, Chen TS, Chen WR (2007) Mechanistic study of apoptosis induced by high-fluence low-power laser irradiation using fluorescence imaging techniques. J Biomed Opt 12(31):064015

37.

Morales JA, Ruiz-Gómez MJ, Gil-Carmona L, Souniron A, Martinez-Morillo M (1995) He-Ne Laser Has No Effect on Cell Cycle Phases of Human Colon Adenocarcinoma Cells. Rev Esp Fisiol 51(33):43–48

38.

Azevedo LH, de Paula Eduardo F, Moreira MS, de Paula Eduardo C, Marques MM (2006) Influence of different power densities of LILT on cultured human fibroblast growth: a pilot study. Lasers Med Sci 2:86–89CrossRef

39.

van Breugel HH, Bar PR (1992) Power density and exposure time of He-Ne laser irradiation are more important than total energy dose in photo-biomodulation of human fibroblasts in vitro. Lasers Surg Med 5:528–537CrossRef

40.

Thornberry NA, Lazebnik Y (1998) Caspases: enemies within. Science 281(32):1312–1316

Title: The effect of low-level laser to apoptosis of chondrocyte and caspases expression, including caspase-8 and caspase-3 in rabbit surgery-induced model of knee osteoarthritis
Authors: Hai-Dan Lin
Cheng-Qi He
Qing-Lu Luo
Jing-Long Zhang
De-Xin Zeng
Publication date: 01-03-2012
Publisher: Springer-Verlag
Published in: Rheumatology International / Issue 3/2012
Print ISSN: 0172-8172
Electronic ISSN: 1437-160X
DOI: https://doi.org/10.1007/s00296-010-1629-5

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