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Journal of Bone and Mineral Metabolism
Published in: Journal of Bone and Mineral Metabolism 5/2012

01-09-2012 | Short communication

Low intensity ultrasound stimulates osteoblast migration at different frequencies

Authors: Jennifer Man, Richard M. Shelton, Paul R. Cooper, Gabriel Landini, Ben A. Scheven

Published in: Journal of Bone and Mineral Metabolism | Issue 5/2012

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Abstract

This study investigated the effects of different frequencies of low intensity ultrasound on osteoblast migration using an in vitro scratch-wound healing assay. Mouse calvarial-derived MC3T3-E1 osteoblasts in culture were exposed to continuous 45 kHz ultrasound (25 mW/cm2) or pulsed 1 MHz ultrasound (250 mW/cm2) for 30 min followed by 2 days’ culture. Ultrasound treatment with either kHz or MHz output similarly and significantly increased cell numbers after 2 days in culture compared with untreated control cultures. In the scratch-wound healing assay the presence of the cell proliferation inhibitor mitomycin C (MMC) did not influence scratch-wound closure in control cultures indicating that cell migration was responsible for the in vitro wound healing. Application of ultrasound significantly stimulated wound closure. MMC did not affect kHz-stimulated in vitro wound healing; however, MMC reduced in part the scratch-wound closure rate in MHz-treated cultures suggesting that enhanced cell proliferation as well as migration was involved in the healing promoted by MHz ultrasound. In conclusion, both continuous kHz and pulsed MHz ultrasound promoted osteoblastic migration; however, subtle differences were apparent in the manner the different ultrasound regimens enhanced in vitro scratch-wound healing.
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Metadata
Title
Low intensity ultrasound stimulates osteoblast migration at different frequencies
Authors
Jennifer Man
Richard M. Shelton
Paul R. Cooper
Gabriel Landini
Ben A. Scheven
Publication date
01-09-2012
Publisher
Springer Japan
Published in
Journal of Bone and Mineral Metabolism / Issue 5/2012
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI
https://doi.org/10.1007/s00774-012-0368-y

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