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Inflammation Research
Published in: Inflammation Research 9/2016

01-09-2016 | Original Research Paper

Longitudinal study of experimental induction of AA amyloidosis in mice seeded with homologous and heterologous AA fibrils

Authors: Naeem Muhammad, Tomoaki Murakami, Yasuo Inoshima, Naotaka Ishiguro

Published in: Inflammation Research | Issue 9/2016

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Abstract

Objective

To investigate pathogenesis and kinetics of experimentally induced murine AA amyloidosis seeded with homologous (murine) and heterologous (bovine) AA fibrils.

Methods

Experimental AA amyloidosis was induced by administration of inflammatory stimulus and preformed AA fibrils to a total of 111 female C57/Black mice. In this longitudinal study, heterologous (bovine) as well as homologous (murine) AA fibrils were injected intraperitoneally to mice in various combinations. Re-stimulation was done at 120 or 300 days post first inoculation. To analyze the intensity of amyloid depositions in mice organs, immunohistochemical techniques and image J software were used. Assessment of cytokines level in sera was done using a Mouse Th1/Th2/Th17 Cytokine CBA Kit.

Results

Incidence and severity of AA amyloidosis were quite low in mice inoculated with heterologous bovine AA fibrils than homologous murine one. Homologous AA fibrils administration at first and second inoculation caused maximum amount of amyloid depositions and severe systemic form of amyloidosis. Increase in the level of pro-inflammatory cytokine IL-6 was observed after first inoculation, while second inoculation caused a further increase in the level of anti-inflammatory cytokine IL-10.

Conclusions

AA amyloidosis can be induced by heterologous as well as homologous AA fibrils. Severity of AA amyloidosis induced with homologous AA fibrils is higher compared to heterologous AA fibrils.
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Metadata
Title
Longitudinal study of experimental induction of AA amyloidosis in mice seeded with homologous and heterologous AA fibrils
Authors
Naeem Muhammad
Tomoaki Murakami
Yasuo Inoshima
Naotaka Ishiguro
Publication date
01-09-2016
Publisher
Springer International Publishing
Published in
Inflammation Research / Issue 9/2016
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-016-0950-1

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