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Magnetic Resonance Materials in Physics, Biology and Medicine
Published in: Magnetic Resonance Materials in Physics, Biology and Medicine 1/2019

01-02-2019 | Research Article

Longitudinal 19F magnetic resonance imaging of brain oxygenation in a mouse model of vascular cognitive impairment using a cryogenic radiofrequency coil

Authors: Ahmed A. Khalil, Susanne Mueller, Marco Foddis, Larissa Mosch, Janet Lips, Ingo Przesdzing, Sebastian Temme, Ulrich Flögel, Ulrich Dirnagl, Philipp Boehm-Sturm

Published in: Magnetic Resonance Materials in Physics, Biology and Medicine | Issue 1/2019

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Abstract

Introduction

We explored the use of a perfluoro-15-crown-5 ether nanoemulsion (PFC) for measuring tissue oxygenation using a mouse model of vascular cognitive impairment.

Methods

Seventeen C57BL/6 mice underwent stereotactic injection of PFC coupled to a fluorophore into the striatum and corpus callosum. Combined 1H/19F magnetic resonance imaging (MRI) to localize the PFC and R1 mapping to assess pO2 were performed. The effect of gas challenges on measured R1 was investigated. All mice then underwent bilateral implantation of microcoils around the common carotid arteries to induce global cerebral hypoperfusion. 19F-MRI and R1 mapping were performed 1 day, 1 week, and 4 weeks after microcoil implantation. In vivo R1 values were converted to pO2 through in vitro calibration. Tissue reaction to the PFC was assessed through ex vivo immunohistochemistry of microglial infiltration.

Results

R1 increased with increasing oxygen concentrations both in vitro and in vivo and the strength of the 19F signal remained largely stable over 4 weeks. In the two mice that received all four scans, tissue pO2 decreased after microcoil implantation and recovered 4 weeks later. We observed infiltration of the PFC deposits by microglia.

Discussion

Despite remaining technical challenges, intracerebrally injected PFC is suitable for monitoring brain oxygenation in vivo.
Appendix
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Metadata
Title
Longitudinal 19F magnetic resonance imaging of brain oxygenation in a mouse model of vascular cognitive impairment using a cryogenic radiofrequency coil
Authors
Ahmed A. Khalil
Susanne Mueller
Marco Foddis
Larissa Mosch
Janet Lips
Ingo Przesdzing
Sebastian Temme
Ulrich Flögel
Ulrich Dirnagl
Philipp Boehm-Sturm
Publication date
01-02-2019
Publisher
Springer International Publishing
Published in
Magnetic Resonance Materials in Physics, Biology and Medicine / Issue 1/2019
Print ISSN: 0968-5243
Electronic ISSN: 1352-8661
DOI
https://doi.org/10.1007/s10334-018-0712-x

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