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01-06-2021 | Original Article

Localization of interleukin-6 signaling complex in epithelialized apical lesions of endodontic origin

Authors: C. Schweitzer, M. Garrido, R. Paredes, C. Stoore, M. Reyes, R. Bologna-Molina, A. Fernández, Marcela Hernández Rios

Published in: Clinical Oral Investigations | Issue 6/2021

Abstract

Objectives

We aimed to determine the immunolocalization patterns of the interleukin (IL)-6 signaling complex in epithelialized and non-epithelialized apical lesions of endodontic origin (ALEOs).

Materials and methods

Epithelialized (n = 8) and non-epithelialized (n = 7) ALEOs were obtained from teeth with indication of extraction in patients with clinical diagnosis of apical periodontitis. All tissues were subjected to routine processing for histopathologic examination and primary antibodies for IL-6, IL-6 receptor (R), and glycoprotein (gp)-130 were used for immunohistochemistry and double immunofluorescence co-localization.

Results

IL-6, IL-6R, and gp-130 were immunolocalized in endothelial cells and mononuclear leukocytes in a diffuse pattern within the connective tissue of epithelialized and non-epithelialized ALEOs. In the epithelialized lesions, two different patterns were identified: IL-6 signaling complex was localized within the proliferating epithelium in a diffuse intracellular pattern and in a cell membrane localization pattern within the mature epithelial lining, showing a decreased intensity towards the surface layers.

Conclusions

IL-6, IL-6R, and gp-130 localized to mononuclear inflammatory cells, vascular endothelial cells, and immature proliferating epithelia in a diffuse pattern and in mature lining epithelia in a localized cell membrane pattern, supporting a role for epithelial proliferation during cyst formation. Additional cell membrane co-localization of IL-6 receptor complex suggests classic signaling involvement in addition to trans-signaling.

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Title: Localization of interleukin-6 signaling complex in epithelialized apical lesions of endodontic origin
Authors: C. Schweitzer
M. Garrido
R. Paredes
C. Stoore
M. Reyes
R. Bologna-Molina
A. Fernández
Marcela Hernández Rios
Publication date: 01-06-2021
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 6/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03738-y

At a glance: The STEP trials

Springer Medicine

Localization of interleukin-6 signaling complex in epithelialized apical lesions of endodontic origin

Abstract

Objectives

Materials and methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

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