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European Journal of Trauma and Emergency Surgery

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Open Access 04-04-2021 | Original Article

Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice

Authors: Katharina Sommer, Heike Jakob, Tobias Kisch, Dirk Henrich, Ingo Marzi, Johannes Frank, Anna L. Sander

Published in: European Journal of Trauma and Emergency Surgery | Issue 3/2022

Abstract

Introduction

Stem cell transplantation is one of the most promising strategies to improve healing in chronic wounds as systemic administration of endothelial progenitor cells (EPC) enhances healing by promoting neovascularization and homing though a high amount of cells is needed. In the following study, we analysed whether local application can reduce the number of EPC needed achieving the same beneficial effect on wound healing.

Material and Methods

Wound healing after local or systemic treatment with EPC was monitored in vivo by creating standardized wounds on the dorsum of hairless mice measuring wound closure every second day. Systemic group received 2 × 10⁶ EPC i.v. and locally treated group 2 × 10⁵ EPC, locally injected. As control PBS injection was performed the same way. Expression of CD31, VEGF, CD90 and, SDF-1α was analysed immunohistochemically for evaluation of neovascularisation and amelioration of homing.

Results

Local (7.1 ± 0.45 SD) as well as systemic (6.1 ± 0.23 SD) EPC transplantation led to a significant acceleration of wound closure compared to controls (PBS local: 9.7 ± 0.5 SD, PBS systemic 10.9 ± 0.38 SD). Systemic application enhanced CD31 expression on day 6 after wounding and local EPC on 6 and 9 in comparison to control. VEGF expression was not significantly affected. Systemic and local EPC treatment resulted in a significantly enhanced SDF-1α and CD90 expression on all days investigated.

Conclusion

Local as well as systemic EPC treatment enhances wound healing. Moreover, beneficial effects are obtained with a tenfold decrease number of EPC when applied locally. Thus, local EPC treatment might be more convenient way to enhance wound healing as number of progenitor cells is limited.

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Title: Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice
Authors: Katharina Sommer
Heike Jakob
Tobias Kisch
Dirk Henrich
Ingo Marzi
Johannes Frank
Anna L. Sander
Publication date: 04-04-2021
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Trauma and Emergency Surgery / Issue 3/2022
Print ISSN: 1863-9933
Electronic ISSN: 1863-9941
DOI: https://doi.org/10.1007/s00068-021-01621-3

At a glance: The STEP trials

Springer Medicine

Local application reduces number of needed EPC for beneficial effects on wound healing compared to systemic treatment in mice

Abstract

Introduction

Material and Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Introduction

Material and Methods

Results

Conclusion

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