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Journal of Inherited Metabolic Disease
Published in: Journal of Inherited Metabolic Disease 1/2015

01-01-2015 | Complex Lipids

Lipidomic analysis of cerebrospinal fluid by mass spectrometry–based methods

Authors: Benoit Colsch, Alexandre Seyer, Samia Boudah, Christophe Junot

Published in: Journal of Inherited Metabolic Disease | Issue 1/2015

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Abstract

Lipids are natural substances found in all living organisms. Essential to the integrity of cell membranes, they also have many biological functions linked to energy storage and cell signaling, and are involved in a large number of heterogeneous diseases such as cancer, diabetes, neurological disorders, and inherited metabolic diseases. Lipids are challenging to analyze because of their huge structural diversity and numerous species. Up to now, lipid analysis has been achieved by targeted approaches focusing on selected families and relying on extraction protocols and chromatographic methods coupled to various detectors including mass spectrometry. Thanks to the technological improvements achieved in the fields of chromatography, high-resolution mass spectrometry and bioinformatics, it is possible to perform global lipidomic analyses enabling the concomitant detection, identification and relative quantification of many lipid species belonging to different families. The aim of this review is to focus on mass spectrometry–based methods to perform lipid and lipidomic analyses and on their application to the analysis of cerebrospinal fluid.
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Metadata
Title
Lipidomic analysis of cerebrospinal fluid by mass spectrometry–based methods
Authors
Benoit Colsch
Alexandre Seyer
Samia Boudah
Christophe Junot
Publication date
01-01-2015
Publisher
Springer Netherlands
Published in
Journal of Inherited Metabolic Disease / Issue 1/2015
Print ISSN: 0141-8955
Electronic ISSN: 1573-2665
DOI
https://doi.org/10.1007/s10545-014-9798-0

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