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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 5/2016

Open Access 01-12-2016 | Translational Research and Biomarkers

Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer

Authors: Yukako Mokutani, MD, Mamoru Uemura, MD, PhD, Koji Munakata, MD, PhD, Daisuke Okuzaki, PhD, Naotsugu Haraguchi, MD, PhD, Hidekazu Takahashi, MD, PhD, Junichi Nishimura, MD, PhD, Taishi Hata, MD, PhD, Kohei Murata, MD, PhD, Ichiro Takemasa, MD, PhD, Tsunekazu Mizushima, MD, PhD, Yuichiro Doki, MD, PhD, Masaki Mori, MD, PhD, Hirofumi Yamamoto, MD, PhD

Published in: Annals of Surgical Oncology | Special Issue 5/2016

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Abstract

Background

Given the role of microRNA in colorectal cancer (CRC) progression, we explored the association between microRNA (miRNA) expression and CRC-related prognosis.

Methods

Three types of tissue samples (primary CRC lesions without liver metastasis, primary lesions with liver metastasis, and liver metastatic tissues) were used for miRNA profiling to identify differentially expressed miRNA. Quantitative real-time PCR was used to examine miRNA expression in CRC cells and in tumor tissues.

Results

MiR-132 was significantly down-regulated in primary CRC tissues with liver metastasis and liver metastatic lesions compared to primary lesions without liver metastasis. Multivariate analysis for overall survival indicated that low miR-132 expression was an independent prognostic factor for CRC patients (overall survival P = 0.040, disease-free survival P = 0.015). Ectopic expression of miR-132 significantly inhibited cell proliferation and cell invasion. The luciferase reporter assay revealed that anoctamin 1 (ANO1) was a direct target of miR-132. Kaplan–Meier survival curves showed that high ANO1 expression was a significant prognostic factor for overall survival of patients with CRC (P = 0.0344).

Conclusions

Down-regulation of miR-132 is associated with poor prognosis in CRC. ANO1 could be one of the crucial targets of miR-132 in CRC.
Appendix
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Metadata
Title
Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer
Authors
Yukako Mokutani, MD
Mamoru Uemura, MD, PhD
Koji Munakata, MD, PhD
Daisuke Okuzaki, PhD
Naotsugu Haraguchi, MD, PhD
Hidekazu Takahashi, MD, PhD
Junichi Nishimura, MD, PhD
Taishi Hata, MD, PhD
Kohei Murata, MD, PhD
Ichiro Takemasa, MD, PhD
Tsunekazu Mizushima, MD, PhD
Yuichiro Doki, MD, PhD
Masaki Mori, MD, PhD
Hirofumi Yamamoto, MD, PhD
Publication date
01-12-2016
Publisher
Springer International Publishing
Published in
Annals of Surgical Oncology / Issue Special Issue 5/2016
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-016-5133-3

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