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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 4/2014

01-12-2014 | Translational Research and Biomarkers

Clinical Significance of GAB2, a Scaffolding/Docking Protein Acting Downstream of EGFR in Human Colorectal Cancer

Authors: Tae Matsumura, MD, Keishi Sugimachi, MD, PhD, Yusuke Takahashi, MD, PhD, Ryutaro Uchi, MD, Genta Sawada, MD, Masami Ueda, MD, Hidenari Hirata, MD, Shotaro Sakimura, MD, Hiroki Ueo, MD, Yuki Takano, MD, Junji Kurashige, MD, PhD, Yoshiaki Shinden, MD, Hidetoshi Eguchi, MD, PhD, Tomoya Sudo, MD, PhD, Hirofumi Yamamoto, MD, PhD, Yuichiro Doki, MD, PhD, Masaki Mori, MD, PhD, FACS, Koshi Mimori, MD, PhD

Published in: Annals of Surgical Oncology | Special Issue 4/2014

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Abstract

Purpose

Recent studies indicated that the scaffolding adaptor protein GAB2 (GRB2-associated binding protein 2) plays a critical role in the proliferation and migration of various cancers. This study aimed to determine the role of aberrant GAB2 expression in human colorectal cancer (CRC).

Methods

Quantitative real-time reverse transcription polymerase chain reaction was used to evaluate GAB2 mRNA expression in 152 CRC tissues samples to determine the clinicopathological significance of GAB2 expression. We also performed in vitro proliferation assays using siGAB2-transfected CRC cells.

Results

GAB2 expression in tumor colorectal tissues was significantly higher than in normal colorectal tissues (p = 0.0212). High GAB2 expression levels were associated with malignant clinicopathologic potential factors, including lymphatic invasion (p = 0.0003), venous invasion (p = 0.0170), and liver metastasis (p = 0.0144). The survival rate of patients with high GAB2 expression levels was significantly lower than that of patients with low GAB2 expression (p = 0.0074). Multivariate analysis indicated that GAB2 expression was a factor affecting lymph node metastasis. Cell proliferation was significantly suppressed by siGAB2 expression in CRC cells in vitro.

Conclusions

GAB2 expression was associated with lymph node metastasis and may play a role in the growth and metastasis of CRC. These results suggest that GAB2 is a potential therapeutic target in CRC.
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Metadata
Title
Clinical Significance of GAB2, a Scaffolding/Docking Protein Acting Downstream of EGFR in Human Colorectal Cancer
Authors
Tae Matsumura, MD
Keishi Sugimachi, MD, PhD
Yusuke Takahashi, MD, PhD
Ryutaro Uchi, MD
Genta Sawada, MD
Masami Ueda, MD
Hidenari Hirata, MD
Shotaro Sakimura, MD
Hiroki Ueo, MD
Yuki Takano, MD
Junji Kurashige, MD, PhD
Yoshiaki Shinden, MD
Hidetoshi Eguchi, MD, PhD
Tomoya Sudo, MD, PhD
Hirofumi Yamamoto, MD, PhD
Yuichiro Doki, MD, PhD
Masaki Mori, MD, PhD, FACS
Koshi Mimori, MD, PhD
Publication date
01-12-2014
Publisher
Springer US
Published in
Annals of Surgical Oncology / Issue Special Issue 4/2014
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-014-3889-x

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